Colorimetric determination of tumor cells via peroxidase-like activity of a cell internalizable nanozyme: Hyaluronic acid attached-silica microspheres containing accessible magnetite nanoparticles

Kip, Çiğdem; Akbay, Esin; Gökçal, Burcu; Savaş, Bürge Okyay; Onur, Mehmet Alį; Tuncel, Alï

doi:10.1016/j.colsurfa.2020.124812

Cited by 15 publications

(7 citation statements)

References 45 publications

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“…Tuncel and colleagues have developed a rapid colorimetric method to detect tumor cells that utilizes an externalizable complex of monodisperse-porous silica microsphere that contains immobilized Fe 3 O 4 NPs (Fe 3 O 4 @SiO 2 microspheres). After combination with hyaluronic acid (HA), a ligand sensitive to CD44 receptors on tumor cells, the nanocomposites could be taken up by human cervical cancer (HeLa) cells and primary brain tumor cells T98 G cells via pinocytosis, with the cell detection achieved by oxidation of TMB generated by the catalytic properties of the Fe 3 O 4 @SiO 2 microspheres [ 166 ]. The detection of cancer cells is achieved by the evaluation of the over-expressed surface biomarkers (e.g., CD44 receptors, folic acid [ 167 ]) or the amount of internalization of nanozymes that caused changes in cancer cells.…”

Section: Nanozymes and Their Potential Applications In Biosensingmentioning

confidence: 99%

Nanozymes—Hitting the Biosensing “Target”

Darland

Zhao

2021

Sensors

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Nanozymes are a class of artificial enzymes that have dimensions in the nanometer range and can be composed of simple metal and metal oxide nanoparticles, metal nanoclusters, dots (both quantum and carbon), nanotubes, nanowires, or multiple metal-organic frameworks (MOFs). They exhibit excellent catalytic activities with low cost, high operational robustness, and a stable shelf-life. More importantly, they are amenable to modifications that can change their surface structures and increase the range of their applications. There are three main classes of nanozymes including the peroxidase-like, the oxidase-like, and the antioxidant nanozymes. Each of these classes catalyzes a specific group of reactions. With the development of nanoscience and nanotechnology, the variety of applications for nanozymes in diverse fields has expanded dramatically, with the most popular applications in biosensing. Nanozyme-based novel biosensors have been designed to detect ions, small molecules, nucleic acids, proteins, and cancer cells. The current review focuses on the catalytic mechanism of nanozymes, their application in biosensing, and the identification of future directions for the field.

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Section: Nanozymes and Their Potential Applications In Biosensingmentioning

confidence: 99%

Nanozymes—Hitting the Biosensing “Target”

Darland

Zhao

2021

Sensors

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“…Since Fe 3 O 4 NPs were found to exhibit intrinsic peroxidase-like activity in 2007 for the first time, numerous nanomaterials have been exploited with enzyme-like properties under the near physiological conditions, which are coined as "nanozymes" [98,99]. Kip et al developed a simple colorimetric method for CTCs detection based on a cell internalizable nanozyme (Figure 5B) [100]. Fe 3 O 4 NPs were immobilized within the porous interiors of silica microspheres and further modified with hyaluronic acid (HA) (HA@Fe 3 O 4 @SiO 2 microspheres).…”

Section: Colorimetric Cytosensorsmentioning

confidence: 99%

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

2021

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The accurate analysis of circulating tumor cells (CTCs) holds great promise in early diagnosis and prognosis of cancers. However, the extremely low abundance of CTCs in peripheral blood samples limits the practical utility of the traditional methods for CTCs detection. Thus, novel and powerful strategies have been proposed for sensitive detection of CTCs. In particular, nanomaterials with exceptional physical and chemical properties have been used to fabricate cytosensors for amplifying the signal and enhancing the sensitivity. In this review, we summarize the recent development of nanomaterials-based optical and electrochemical analytical techniques for CTCs detection, including fluorescence, colorimetry, surface-enhanced Raman scattering, chemiluminescence, electrochemistry, electrochemiluminescence, photoelectrochemistry and so on.

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“…Different systems based on CAC principles were also designed for quantitative detection of tumor cells and quantitative determination of some important biomarkers related to them. Colorimetric determination of human cervical cancer cells and T98G, glioblastoma cells was F I G U R E 8 Schematic representation of the interaction between HA attached-monodisperse-porous silica microspheres containing accessible Fe 3 O 4 nanoparticles as a nanozyme with peroxidase-like activity and HeLa/T98G cells for colorimetric detection of tumor cell concentration [167] performed using cell internalizable nanozyme with peroxidase-like activity. For this purpose, hyaluronic acid attached-silica microspheres containing accessible magnetite nanoparticles were used as the nanozyme (Figure 8) [167].…”

Section: Cell Affinity Capturementioning

confidence: 99%

“…Schematic representation of the interaction between HA attached‐monodisperse‐porous silica microspheres containing accessible Fe 3 O 4 nanoparticles as a nanozyme with peroxidase‐like activity and HeLa/T98G cells for colorimetric detection of tumor cell concentration [167]…”

Section: Bioaffinity Chromatography Techniquesmentioning

confidence: 99%

“…Colorimetric determination of human cervical cancer cells and T98G, glioblastoma cells was performed using cell internalizable nanozyme with peroxidase‐like activity. For this purpose, hyaluronic acid attached‐silica microspheres containing accessible magnetite nanoparticles were used as the nanozyme (Figure 8) [167]. A multiparameter affinity microchip for early sepsis diagnosis was developed by Zhang et al.…”

Section: Bioaffinity Chromatography Techniquesmentioning

confidence: 99%

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Recent trends in sorbents for bioaffinity chromatography

Kip

Hamaloğlu

Demir

et al. 2021

J of Separation Science

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Isolation or enrichment of biological molecules from complex biological samples is mostly a prerequisite in proteomics, genomics, and glycomics. Different techniques have been used to advance the efficiency of the purification of biological molecules. Bioaffinity chromatography is one of the most powerful technique that plays an important role in the isolation of target biological molecules by the specific interactions with ligands that are immobilized on different support materials. This review examines the recent developments in bioaffinity chromatography particularly over the past 5 years in the literature. Also properties of supports, immobilization techniques, types of binding agents, and methods used in bioaffinity chromatography applications are summarized.

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Colorimetric determination of tumor cells via peroxidase-like activity of a cell internalizable nanozyme: Hyaluronic acid attached-silica microspheres containing accessible magnetite nanoparticles

Cited by 15 publications

References 45 publications

Nanozymes—Hitting the Biosensing “Target”

Nanozymes—Hitting the Biosensing “Target”

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

Recent trends in sorbents for bioaffinity chromatography

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