Column-switching high-performance liquid chromatographic analysis of BO-2727, a new carbapenem antibiotic, in human plasma and urine by direct injection

Column-switching high-performance liquid chromatographic method for the determination of zaltoprofen in rat plasma

“…: +82 2 380 1774; fax: +82 2 380 1776. procedures are increasingly used [4]. And especially, columnswitching systems via a switching valve have great advantages in permitting the direct injection of protein rich samples, such as plasma [5][6][7]. In case of non-steroidal anti-inflammatory drugs (NSAIDs) used for the treatment of rheumatoids (RA), some drug substances such as naproxen, ibuprofen and ketoprofen have been reported about the bioanalytical method using column-switching technique [4,[8][9][10][11].…”

“…The column switching technique avoids off-line procedures for sample clean-up [5,6,17]. This method allowed plasma samples to be directly injected onto a fully automated HPLC system, thereby resulting in an accurate assay without internal standards.…”

The application of fully automated on-line solid phase extraction in bioanalysis

“…The challenge has been to clean up these samples sufficiently to allow highly sensitive and robust assays with the minimum of analyst time spent on sample preparation. Previous solutions have involved the use of turbulent flow chromatography [1] or column switching techniques [2,3]. These techniques often suffer from limited sensitivity or analyte carryover, respectively.…”

Column-switching high-performance liquid chromatographic analysis of fluvastatin in rat plasma by direct injection

“…Nowadays, several HPLC methods are described in literature for some drugs in various biological matrices based on direct injection of the samples without sample clean-up [9]. And especially, columnswitching systems via a switching valve have great advantages in permitting the direct injection of protein rich samples such as plasma [10][11][12]. The column-switching technique avoids off-line procedures for sample clean-up [10][11][12][13].…”