Combination of aqueous two-phase flotation and inverse transition cycling: Strategies for separation and purification of recombinant β-glucosidase from cell lysis solution

Han, Juan; Fang, Sihan; He, Xingchen; Wang, Lei; Li, Chunmei; Wu, Jiacong; Cai, Yunfeng; Wang, Yun

doi:10.1016/j.foodchem.2021.131543

Cited by 12 publications

(4 citation statements)

References 31 publications

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“…[59,60] Inverse conversion cycling (ITC) is a technique to purify recombinant proteins based on the thermo-responsive properties of elastin-like peptides (ELPs), where ELP-tagged proteins can be precipitated from fermentation broth when ambient temperature is higher than the phase transition temperature (Tt), resulting in higher purity proteins. Han et al [61] established a method combined ATPF with ITC for efficient purification of recombinant protein β-glucosidase (GLEGB) dependent on graphene-binding tag (GB) and ELP tag from cell lysates. GLEGB is preferentially adsorbed on the nitrogen bubble interface due to the hydrophobicity of GB, entered the top phase of ATPF, and then based on the thermo-responsive properties of the ELP tag, with a final purification ploidy of 24.26 ± 0.22 and the protein remaining structurally stable after six cycles of purification.…”

Section: Dual Aqueous Phase Flotation (Atpf) Combined With Inverse Tr...mentioning

confidence: 99%

“…Han et al. [ 61 ] established a method combined ATPF with ITC for efficient purification of recombinant protein β‐glucosidase (GLEGB) dependent on graphene‐binding tag (GB) and ELP tag from cell lysates. GLEGB is preferentially adsorbed on the nitrogen bubble interface due to the hydrophobicity of GB, entered the top phase of ATPF, and then based on the thermo‐responsive properties of the ELP tag, with a final purification ploidy of 24.26 ± 0.22 and the protein remaining structurally stable after six cycles of purification.…”

Section: Biosynthesis Technology Of Peptide Drugsmentioning

confidence: 99%

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Advances in biosynthesis of peptide drugs: Technology and industrialization

Wang,

Chen,

Qin

et al. 2023

Biotechnology Journal

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Peptide drugs are developed from endogenous or synthetic peptides with specific biological activities. They have advantages of strong target specificity, high efficacy and low toxicity, thus showing great promise in the treatment of many diseases such as cancer, infections and diabetes. Although an increasing number of peptide drugs have entered market in recent years, the preparation of peptide drug substances is yet a bottleneck problem for their industrial production. Comparing to the chemical synthesis method, peptide biosynthesis has advantages of simple synthesis, low cost, and low contamination. Therefore, the biosynthesis technology of peptide drugs has been widely used for manufacturing. Herein, we reviewed the development of peptide drugs and recent advances in peptide biosynthesis technology, in order to shed a light to the prospect of industrial production of peptide drugs based on biosynthesis technology.This article is protected by copyright. All rights reserved

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Section: Dual Aqueous Phase Flotation (Atpf) Combined With Inverse Tr...mentioning

confidence: 99%

Section: Biosynthesis Technology Of Peptide Drugsmentioning

confidence: 99%

Advances in biosynthesis of peptide drugs: Technology and industrialization

Wang,

Chen,

Qin

et al. 2023

Biotechnology Journal

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“…The recovery of WSP is regarded as the inaugural phase in the comprehensive resource recovery process from SWW . At present, foam separation is often considered the most cost-effective strategy for protein recovery . Unfortunately, WSP exhibits poor interfacial stability and tends to aggregate into insoluble particles.…”

Section: Introductionmentioning

confidence: 99%

“…27 At present, foam separation is often considered the most cost-effective strategy for protein recovery. 28 Unfortunately, WSP exhibits poor interfacial stability and tends to aggregate into insoluble particles. Its foams are also prone to coarsening and rupturing, leading to a lower recovery rate of WSP.…”

Section: ■ Introductionmentioning

confidence: 99%

“Microflower-Templated” Janus Sheets: Synthesis and Application in Stabilizing Foams

Zhang,

Ma,

Xue

et al. 2024

Langmuir

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In this study, we proposed a method for fabricating Janus sheets using biological "microflowers" as a sacrificial template. The microflower-templated Janus sheets (MF-JNSs) were employed as a foam stabilizer in foam separation of the whey soybean protein (WSP). The MF-JNSs took inorganic hybrid microflowers (BSA@Cu 3 (PO 4 ) 2 -MF) as template, followed by the sequential attachment of protamine and silica to the surface of the BSA@Cu 3 (PO 4 ) 2 -MF. Subsequently, the template was removed using ethylenediaminetetraacetic acid after the silicon dioxide was modified by 3-(methacryloyloxy) propyl trimethoxysilane. Upon template dissolution, the modified silica layer, lacking support from the core, fractured to form the MF-JNSs. This method omitted the step of treating the hollow ball by external force and obtained Janus sheets in one step, indicating that it was simple and feasible. The morphology, structure, and composition of the MF-JNSs were analyzed by SEM, TEM, AFM, XRD, and FT-IR. The MF-JNSs were found to delay the breakage time of the Pickering emulsion, demonstrating their emulsion stabilizing capability. Importantly, they significantly enhanced the foam half-life and foam height of soybean whey wastewater (SWW). Moreover, the recovery percentage and enrichment ratio of WSP, separated from SWW by foam separation, were improved to 81 ± 0.28 and 1.20 ± 0.05%, respectively.

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