Combination of arabinogalactan and curcumin induces apoptosis in breast cancer cells in vitro and inhibits tumor growth via overexpression of p53 level in vivo

Moghtaderi, Hassan; Sepehri, Houri; Attari, Farnoosh

doi:10.1016/j.biopha.2017.01.072

Cited by 63 publications

(37 citation statements)

References 39 publications

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“…Concisely, the treated cells were washed in phosphate buffer saline (PBS) and incubated with 20 μM DCFD in PBS for 30 minutes at 37 ◦ C. The samples were analyzed for forward- and side-scattering properties, as well as green and red fluorescence intensity using the FACScan flow cytometer (BD FACS Calibur, BD Biosciences, San Jose, USA). 19 …”

Section: Methodsmentioning

confidence: 99%

Gallic acid and curcumin induce cytotoxicity and apoptosis in human breast cancer cell MDA-MB-231

Moghtaderi¹,

Sepehri²,

Delphi³

et al. 2018

Bioimpacts

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Introduction: Gallic acid (GA) and curcumin (Cur) are natural phenolic compounds that their anti-tumor effects on many types of cancers have been proved. In the current study, the effect of the combination of these agents on MDA-MB-231 breast cancer cells was investigated. Methods: Inhibition of cell proliferation (MTT assay), light microscopy, fluorescence microscopy, cell cycle analysis, nitrite detection, ROS levels, measurement of the mitochondrial membrane potential, GSH level, Annexin V assay, RT-PCR and Western blotting methods were applied. Results: The results revealed the combination of GA and Cur strongly decreased MDA-MB-231 cell growth. Moreover, this combination increased ROS level and cytotoxic activity along with the glutathione depletion in MDA-MB-231 cells. Flow cytometry analysis showed the combination of GA and Cur increased sub-G1 cell population. Furthermore, fluorescent staining and Annexin V/PI assay showed that apoptotic cells were significantly increased in the presence of GA and Cur. At last, protein expression evaluation showed that the combination of GA and Cur significantly decreased Bcl-2 level while increased Bax, cleaved-caspase3 and PARP levels in MDA-MB-231 cells. Conclusion: These results suggest that GA in combination with Cur could be a possible candidate for chemoprevention agent of triple negative breast cancer.

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Section: Methodsmentioning

confidence: 99%

Gallic acid and curcumin induce cytotoxicity and apoptosis in human breast cancer cell MDA-MB-231

Moghtaderi¹,

Sepehri²,

Delphi³

et al. 2018

Bioimpacts

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“…Caspase-3 is a major executioner protein of apoptosis which can be activated by both extrinsic and intrinsic pathways. It has been shown that the p53 promotes apoptosis through interactions with Caspase-3 and Bcl-2 family proteins including BAX both in vitro and in vivo in recent studies [ 28 – 30 ]. According to our results, it can be speculated that down-regulation of ESCCAL_1 inhibits ESCC tumor growth might be mediated by Src and p38α pathway.…”

Section: Discussionmentioning

confidence: 99%

Down-regulation of long non-coding RNA ESCCAL_1 inhibits tumor growth of esophageal squamous cell carcinoma in a xenograft mouse model

Cui

et al. 2017

Oncotarget

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Esophageal squamous cell carcinoma (ESCC) is one of the most lethal malignant cancers with high incidence and mortality. Current reliable effective diagnostic and prognostic biomarkers are very limited in clinic. Emerging evidence indicates that dysregulated expression of the long non-coding RNAs (lncRNAs) was examined in various types of cancer including ESCC. ESCC associated lncRNA _1 (ESCCAL_1) was first time identified to be increased expression in ESCC, and therefore named by our research team. However, its potential function in the progression of ESCC remains unclear. In this study, we investigated the effect of ESCCAL_1 knockdown on ESCC tumorigenicity using a xenograft mouse model and explored the underlying molecular mechanism. Here we showed that ESCCAL_1 knockdown significantly inhibited EC9706 cell growth in nude mice. Interestingly, we also found that reduced expression of ESCCAL_1 resulted in distinct alterations of relative phosphorylation level of kinases (p-p38α, p-JNK, p-FAK and p-Src), and significant changes of the expression level of apoptosis-related proteins (p53, BAX, Bcl-2 and Caspase-3). In summary, our results suggest that lncRNA ESCCAL_1 is a potential diagnostic and prognostic target of ESCC.

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“…Thus, the shorter and better extraction methods of CUR open new windows in the research of the phytotherapeutic actions of CUR such as: cholagogue action (stimulates bile release), anticancer, inhibition of the development of cancerous tumors and metastases (breast, stomach, colon, lung, liver, skin) [112][113][114][115], anti-inflammatory action, hepatoprotective inclusive in non-alcoholic fatty liver disease [116], antiatherosclerotic, antiplatelet effects but not in cases of vitamin K coagulopathies [117], prevents AD and other neurodegenerative disorders [118,119].…”

Section: Nanoformulation: Molecules Extraction Techniques and Alzheimentioning

confidence: 99%

Curcumin’s Nanomedicine Formulations for Therapeutic Application in Neurological Diseases

Salehi

Calina

Docea

et al. 2020

JCM

138

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The brain is the body’s control center, so when a disease affects it, the outcomes are devastating. Alzheimer’s and Parkinson’s disease, and multiple sclerosis are brain diseases that cause a large number of human deaths worldwide. Curcumin has demonstrated beneficial effects on brain health through several mechanisms such as antioxidant, amyloid β-binding, anti-inflammatory, tau inhibition, metal chelation, neurogenesis activity, and synaptogenesis promotion. The therapeutic limitation of curcumin is its bioavailability, and to address this problem, new nanoformulations are being developed. The present review aims to summarize the general bioactivity of curcumin in neurological disorders, how functional molecules are extracted, and the different types of nanoformulations available.

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Combination of arabinogalactan and curcumin induces apoptosis in breast cancer cells in vitro and inhibits tumor growth via overexpression of p53 level in vivo

Cited by 63 publications

References 39 publications

Gallic acid and curcumin induce cytotoxicity and apoptosis in human breast cancer cell MDA-MB-231

Gallic acid and curcumin induce cytotoxicity and apoptosis in human breast cancer cell MDA-MB-231

Down-regulation of long non-coding RNA ESCCAL_1 inhibits tumor growth of esophageal squamous cell carcinoma in a xenograft mouse model

Curcumin’s Nanomedicine Formulations for Therapeutic Application in Neurological Diseases

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