Combination of arbuscular mycorrhizal fungi and phosphate solubilizing bacteria on growth and production of Helianthus tuberosus under field condition

Abstract

In this work, the effects of co-inoculation between an arbuscular mycorrhizal fungus (AMF) and a phosphate solubilizing bacteria (PSB) to promote the growth and production of sunchoke under field condition were investigated during 2016 and 2017. Four treatments were set up as follows: plants without inoculation, with AMF inoculation, with PSB inoculation and with co-inoculation of PSB and AMF. The results showed the presence of PSB and AMF colonization at the harvest stage in both years. This suggested the survival of PSB and successful AMF colonization throughout the experiments. According to correlation analysis, PSB positively affected AMF spore density and colonization rate. Also, both AMF and PSB positively correlated with growth and production of sunchoke. Co-inoculation could enhance various plant parameters. However, better results in 2016 were found in co-inoculation treatment, while AMF inoculation performed the best in 2017. All of these results suggested that our AMF and PSB could effectively promote growth and production of sunchoke under field conditions. Such effects were varied due to different environmental conditions each year. Note that this is the first study showing successful co-inoculation of AMF and PSB for promoting growth and yield of sunchoke in the real cultivation fields.

Isolation and identification of indigenous Arbuscular Mycorrhizal Fungi (AMF) of forest clove rhizosphere from Maluku, Indonesia

“…Plants associated with mycorrhizae perform as essential carbon sources for mycorrhizal growth (Wang et al 2017). AMF plays a role in increasing the absorption of nutrients, especially phosphorus, making soil aggregates more stable, as a bio-regulatory agent, increasing groundwater absorption and as a biological control agent (biocontrol) as well as being able to absorb and store heavy metals in the vacuoles, vesicles, and hyphae (Prayudyaningsih et al 2019;Setyaningsih et al 2020;Chaiya et al 2021;Ishaq et al 2021;Meng et al 2021;Nacoon et al 2021;Wu et al 2021).…”

“…The different types and densities of AMF spores in the two regions were strongly influenced by environmental factors, especially climate and soil (Alimi et al 2021;Meng et al 2021;Nacoon et al 2021;Piliarová et al 2019). Piliarová et al (2019) and Nacoon et al (2021) stated that the presence of AMF is strongly influenced by the type of host plant, spore type, and environmental factors such as soil. Soil is the most influential factor in the different types and spore density (Table 2).…”

“…Soil is the most influential factor in the different types and spore density (Table 2). Soil factors such as soil texture and structure, N and P nutrients, water, and pH significantly affect the distribution of mycorrhizae (Husna et al 2015;Lara-Capistran et al 2021;Nacoon et al 2021;Piliarová et al 2019).…”

“…Arbuscular mycorrhizal fungi (AMF) are symbiotic with plants (Wang et al 2017;Meng et al 2021). AMF can facilitate the uptake of mineral nutrients such as phosphorus and nitrogen by increasing the absorption surface area and mobilizing nutrients (Wang et al 2017;Ishaq et al 2021;Nacoon et al 2021).…”

“…AMF is classified into the phylum Glomeromycota, class Glomeromycetes, four orders Glomerales, Diversisporales, Paraglomerales, Archaeosporales with 11 families, and 17 genera (Tuheteru et al 2019;Ibou et al 2021). AMF can be used as a biological agent because it assists nutrient absorption, protection against root pathogens infections, increasing water availability for plants, and increasing growth hormones (Setyaningsih et al 2020;Ishaq et al 2021;Meng et al 2021;Nacoon et al 2021). The use of AMF in cloves has previously been reported by Putri et al (2016) by using mycorrhizal micover.…”