Combination of BCL11A siRNA with vincristine increases the apoptosis of SUDHL6 cells

Abstract

BackgroundB cell chronic lymphocytic leukemia/lymphoma 11 A (BCL11A) is associated with human B cell malignancy initiation. Our previous study has shown that downregulation of BCL11A mRNA by small interfering RNA (siRNA) is capable of inducing apoptosis in the SUDHL6 cell line. To further explore the effects of BCL11A siRNA on the enhanced cytotoxicity of a chemotherapeutic drug, we investigated the effects of BCL11A siRNA combined with vincristine (VCR) on SUDHL6 cell proliferation and apoptosis.MethodsChemically synthesized BCL11A siRNA was transfected into SUDHL6 cells using the HiPerFect Transfection Reagent in combination with VCR. Cell proliferation was measured by the CCK8 assay. The morphology of apoptotic cells was observed with Hoechst 33258 staining. The rate of cell apoptosis was determined by annexin V-fluorescein isothiocyanate/propidium iodide double staining using fluorescence-activated cell sorting (FACS) analysis.ResultsAfter BCL11A siRNA plus VCR treatment, cell proliferation was significantly decreased in comparison with VCR or BCL11A siRNA treatment alone and negative control siRNA plus VCR treatment (P <0.05). The apoptotic rate of BCL11A siRNA plus VCR treated cells was significantly increased compared with BCL11A siRNA and VCR treatment alone and negative control siRNA plus VCR treatment (P <0.05).ConclusionsThe combination of BCL11A siRNA and VCR increases apoptosis in SUDHL6 cells. Our study implies that BCL11A siRNA in combination with VCR may be a useful approach for improving effective treatment for B cell lymphoma.

BCL11A confers cell invasion and migration in androgen receptor‑positive triple‑negative breast cancer

“…On the basis of previous results, in TNBC cells, disrupting BCL11A expression significantly reduced cell proliferation and invasion. Additionally, gene expression profiling of BCL11A siRNA-treated SUDHL6 cells proved that BCL11A could be associated with the signaling network of cell cycle (22). However, the present data did not indicate a relationship between BCL11A and cell cycle in TNBC.…”

“…Notably, BCL11A may also promote stemness in breast cancer cells by inducing Wnt/β-catenin signaling (21). BCL11A activation is associated with the induction of numerous carcinogenic signaling pathways, and there is growing evidence to suggest that BCL11A downregulation inhibits tumorigenesis (22,23). However, the specific functions of BCL11A and the underlying mechanism of its involvement in TNBC have not been fully clarified.…”

Cell cycle kinetics, apoptosis rates and gene expressions of MDR‐1, TP53, BCL‐2 and BAX in transmissible venereal tumour cells and their association with therapy response

“…Taking into account the above data, the description of vincristine antitumour potential varies according to concentration, exposure time, and the number of cells in the mitosis phase during the exposure period . Researchers using the drug for solid tumour treatment, and other cell lines of different TVT, also described high percentages of apoptosis . In addition, Shi et al , when comparing various cancer cell lines observed that death, as a response to antimitotic treatment, varies widely …”

The expression pattern of Bcl11a, Mdm2 and Pten genes in B‐cell acute lymphoblastic leukemia

“…Although the expression pattern of Bcl11a gene has been described in B‐cell lymphomas and B‐CLL, little is known about the expression character of Bcl11a gene in B‐ALL. In this study, Bcl11a and Mdm2, Pten mRNA expression in B‐ALL was determined by real‐time quantitative PCR.…”

“…Meanwhile, our previous results have shown that a broad range of genes including murine double minute 2 ( Mdm2 ) and phosphorate and tension homolog ( Pten ) were altered by analyzing the global gene expression profile in the diffuse large B‐cell lymphoma cell line SUDHL6 after Bcl11a downregulation . Our previous study has also showed that Bcl11a was related to apoptosis and proliferation of B lymphoma cells …”