Combination of chemotherapy and Au-nanoparticle photothermy in the visible light to tackle doxorubicin resistance in cancer cells

Abstract

Despite great advances in the fight against cancer, traditional chemotherapy has been hindered by the dose dependent adverse side effects that reduce the usable doses for effective therapy. This has been associated to drug resistance in tumor cells that often cause relapse and therapy failure. These drawbacks have been tackled by combining different therapeutic regiments that prevent drug resistance while decreasing the chemotherapy dose required for efficacious ablation of cancer. In fact, new metallic compounds have been in a continuous development to extend the existing chemotherapy arsenal for these combined regimens. Here, we demonstrate that combination of a metallic compound (TS265), previously characterized by our group, with photothermy circumvents cells resistant to Doxorubicin (DOX). We first engendered a colorectal carcinoma cell line (HCT116) highly resistant to DOX, whose viability was diminished after administration of TS265. Cancer cell death was potentiated by challenging these cells with 14 nm spherical gold nanoparticles followed by laser irradiation at 532 nm. The combination of TS265 with photothermy lead to 65% cell death of the DOX resistant cells without impacting healthy cells. These results support the use of combined chemotherapy and photothermy in the visible spectrum as an efficient tool for drug resistant tumors.

Microscale direct measurement of localized photothermal heating in tissue-mimetic hydrogels

“…In this study, for the favorable penetration depth in tissue, we selected the photothermal agents (GNRs) in NIR-I biological window (lowest absorption coefficient for water and hemoglobin 58 ). The presented technique is also applicable with other NPs operating in visible light 59 or IR regions 60,61 in spectrum. Finally, our developed system for localized photothermal investigations is compatible with other local hyperthermia techniques such as magnetic hyperthermia [62][63][64] , ultrasound hyperthermia 65,66 and microwave hyperthermia 67,68 with minor modifications.…”

Theranostic Nanoparticles and Their Spectrum in Cancer

“…Spherical silver nanoparticles ensure anti-inflammatory potential [54] and promote camptothecin apoptotic activity in cervical cancer [55]. Despite the advantages offered by silver nanoparticles, progress in spherical gold nanoparticles makes possible their use for combined therapies like drug delivery and photothermy [56].…”

Evaluation the synergistic antitumor effect of methotrexate–camptothecin codelivery prodrug from self‐assembly process to acid‐catalyzed both drugs release: A comprehensive theoretical study

“…Therefore, combination chemotherapy is becoming increasingly important for achieving more efficiency, decreasing the limitation of single‐drug chemotherapy (e.g., dosage) and reducing drug resistance . The improved efficiency of combination chemotherapy, which uses multidrug, in comparison to single‐agent treatment is derived from a variety of reasons: initial resistance to any single‐drug is frequent; multiple anticancer drugs having different mechanisms of action allow independent cell killing by each agent; minimize drug dose to reduce toxicity and improve the synergistic effect . Recently, nanotechnology in cancer therapy based on combination chemotherapy has made great strides.…”