Combination of flow injection with electrophoresis using capillaries and chips

Chen, Yonglei; Lü, Wenhui; Chen, Xingguo; Hu, Zhide

doi:10.1002/elps.200600587

Cited by 18 publications

(11 citation statements)

References 43 publications

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“…There have been a number of publications on in-situ CE systems [103], comparable with that discussed by Hauser's group. These often consist of portable CE instruments which are modified (or adapted) such that they automatically obtain samples.…”

Section: In-situ Ce Systemsmentioning

confidence: 71%

Review on the development of truly portable andin-situcapillary electrophoresis systems

Lewis

Cranny

Harris

et al. 2013

Meas. Sci. Technol.

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Capillary electrophoresis (CE) is a technique which uses an electric field to separate a mixed sample into its constituents. Portable CE systems enable this powerful analysis technique to be used in the field. Many of the challenges for portable systems are similar to those of autonomous in-situ analysis and therefore portable systems may be considered a stepping stone towards autonomous in-situ analysis. CE is widely used for biological and chemical analysis and example applications include: water quality analysis; drug development and quality control; proteomics and DNA analysis; counter-terrorism (explosive material identification) and corrosion monitoring. The technique is often limited to laboratory use, since it requires large electric fields, sensitive detection systems and fluidic control systems. All of these place restrictions in terms of: size, weight, cost, choice of operating solutions, choice of fabrication materials, electrical power and lifetime. In this review we bring together and critique the work by researchers addressing these issues. We emphasize the importance of a holistic approach for portable and in-situ CE systems and discuss all the aspects of the design. We identify gaps in the literature which require attention for the realization of both truly portable and in-situ CE systems.

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Section: In-situ Ce Systemsmentioning

confidence: 71%

Review on the development of truly portable andin-situcapillary electrophoresis systems

Lewis

Cranny

Harris

et al. 2013

Meas. Sci. Technol.

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“…Ruzicka et al [74] first introduced FI in 1975 and FI for sample introduction was combined with CE in 1997 by Kuban et al [75] and Fang et al [76]. Coupling FI and CE has been reviewed briefly by several groups [77][78][79]. One of the main factors limiting successful combination of FI and CE is the problematic decoupling of the HV applied for CE separation from the FI system.…”

Section: Flow Injection (Fi)mentioning

confidence: 99%

Portable capillary-based (non-chip) capillary electrophoresis

Ryvolová

Preisler

Brabazon

et al. 2010

TrAC Trends in Analytical Chemistry

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Miniaturized, portable instrumentation has been gaining popularity in all areas of analytical chemistry. Capillary electrophoresis (CE), due to its main strengths of high separation efficiency, relatively short analysis time and low consumption of chemicals, is a particularly suitable technique for use in portable analytical instrumentation. In line with the general trend in miniaturization in chemistry utilizing microfluidic chips, the main thrust of portable CE (P-CE) systems development is towards chip-based miniaturized CE. Despite this, capillary-based (non-chip) P-CE systems have certain unmatched advantages, especially in the relative simplicity of the regular cylindrical geometry of the CE capillary, maximal volume-to-surface ratio, no need to design and to fabricate a chip, the low costs of capillary compared to chip, and better performance with some detection techniques. This review presents an overview of the state of the art of P-CE and literature relevant to future developments. We pay particular attention to the development and the potential of miniaturization of functional parts for P-CE. These include components related to sample introduction, separation and detection, which are the key elements in P-CE design. The future of P-CE may be in relatively simple, rugged designs (e.g., using a short piece of capillary fixed to a chip-sized platform on which injection and detection parts can be mounted). Electrochemical detection is well suited for miniaturization, so is probably the most suitable detection technique for P-CE, but optical detection is gaining interest, especially due to miniaturized light sources (e.g., light-emitting diodes).

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“…Flow systems have also been coupled to microchip electrophoretic systems [154], using a capillary as the interface between an SI system and the microchip; for this purpose, one end of the capillary is attached to the microchip via a Teflon fitting. The principal shortcoming of this combination is the presence of residual hydrodynamic flow in the microchip separation channel [154].…”

Section: Robotic Interfacementioning

confidence: 99%