Combination of low-intensity pulsed ultrasound and C3H10T1/2 cells promotes bone-defect healing

He, Rui-Wen; Zhou, Weichen; Zhang, Yu; Zhou, Tianjun; Yu, Haisheng; Luo, Yueping; Bao-ru, Liu; Ran, Jianbo; Wu, Junru; Yan, Wang; Chen, Wenzhi

doi:10.1007/s00264-015-2898-0

Cited by 18 publications

(15 citation statements)

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“…27,30,31 Mesenchymal stem cells have been claimed to have the ability to sense and respond to physical stimuli. 32,33 Several preliminary studies have suggested that LIPUS can act on MSCs and promote their proliferation in vitro, [34][35][36] while some other studies have reported that LIPUS has no effect on or even inhibits the proliferation of stem cells. [37][38][39] It has been reported that p44/p42 extracellular signal-regulated kinases (ERK1/2) signalling pathway can be activated by various mechanical stimuli, 40 and they are crucial signalling molecules in LIPUS-induced cellular responses.…”

Section: Human Amnion-derived Mesenchymal Stem Cells (Had-mscs)mentioning

confidence: 99%

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Low‐intensity pulsed ultrasound activates ERK1/2 and PI3K‐Akt signalling pathways and promotes the proliferation of human amnion‐derived mesenchymal stem cells

Wei

et al. 2017

Cell Proliferation

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Section: Human Amnion-derived Mesenchymal Stem Cells (Had-mscs)mentioning

confidence: 99%

“…Mesenchymal stem cells have been claimed to have the ability to sense and respond to physical stimuli . Several preliminary studies have suggested that LIPUS can act on MSCs and promote their proliferation in vitro , while some other studies have reported that LIPUS has no effect on or even inhibits the proliferation of stem cells …”

Section: Introductionmentioning

confidence: 99%

Low‐intensity pulsed ultrasound activates ERK1/2 and PI3K‐Akt signalling pathways and promotes the proliferation of human amnion‐derived mesenchymal stem cells

Wei

et al. 2017

Cell Proliferation

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“…Therefore, we chose a lower frequency of LIPUS so that its energy could pass through the bone tissue and act on the bone marrow. In a previous study, the temporal and spatial average of acoustic intensity of 30‐mW/cm 2 LIPUS showed a good proliferative effect on BMSCs. Therefore, a preliminary mathematical simulation was performed to estimate whether 30 to 100 mW/cm 2 of energy could reach bone marrow after the US passed through the skin, soft tissue, and cortical bone.…”

Section: Discussionmentioning

confidence: 81%

“…Bone marrow mesenchymal stem cells (BMSCs) and the osteoblasts and endothelial cells differentiated from BMSCs are important components of the hematopoietic microenvironment, providing nutrition and structural support for the normal proliferation and differentiation of hematopoietic stem cells and playing a key regulatory role. Our early study showed that low‐intensity pulsed ultrasound (LIPUS) could promote the proliferation of BMSCs and accelerate the differentiation of BMSCs into osteoblasts and endothelial cells (our unpublished data, 2016). Therefore, we hypothesize that LIPUS could promote the proliferation and differentiation of BMSCs in the bone marrow by acting on the bone marrow, thereby affecting the hematopoietic microenvironment, modulating the hematopoietic function of hematopoietic stem cells, and eventually alleviating myelosuppression.…”

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confidence: 99%

Low‐Intensity Pulsed Ultrasound Relieves Mild and Severe Myelosuppression Induced by Cyclophosphamide in Rabbits

Bao-ru

Zhou

et al. 2019

J of Ultrasound Medicine

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Objective-This study aimed to investigate the effect of low-intensity pulsed ultrasound (LIPUS) on cyclophosphamide (CTX)-induced rabbit myelosuppression.Methods-Rabbits (n = 90) were randomly divided into a mild myelosuppression group (n = 40), a severe myelosuppression group (n = 40), and a normal control group (group Cu 28 ; n = 10). The mild and severe myelosuppression models were established by daily ear vein injection of 15-and 40-mg/kg CTX for 4 continuous days, respectively. Then they were randomly divided into LIPUS groups (A u and B u ) and control groups (A c and B c ). LIPUS was applied once per day for 20 minutes for 7 (A u7 and B u7 ) and 28 (A u28 and B u28 ) days. Physical conditions, mortality, blood cell counts, and bone marrow proliferation were calculated. Erythropoietin interleukin 3, and granulocyte-macrophage colony-stimulating factor levels were measured by an enzyme-linked immunosorbent assay. Flow cytometry was used to detect the granulocyte phagocytosis rate. Hematoxylin-eosin staining was performed to analyze changes of skin and muscle.Results-Compared with the control group, LIPUS improved the number of peripheral blood cells (P < .05) and bone marrow nucleated cells and reduced the mortality of rabbits with myelosuppression of different degrees. Long-term treatment for 28 days had no effect on the levels of erythropoietin, interleukin 3, and granulocyte-macrophage colony-stimulating factor and granulocyte phagocytosis (P > .05). The parts of the skin where LIPUS was applied did not show any burning marks, and the muscle tissue in the path of LIPUS acoustic channels showed no obvious pathologic changes.Conclusions-Low-intensity pulsed ultrasound is a safe and effective method to relieve CTX-induced myelosuppression.Abbreviations BMSC, bone marrow mesenchymal stem cell;

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“…Based on previous studies [25,26] and our pilot experiment, the cells in the LIPUS treated groups (LC, LO, LV and LD) were treated with low-intensity pulsed ultrasound (Chongqing Haifu Medical Technology Co., Ltd., Chongqing, China), setting the frequency at 0.6 MHz, repeat frequency at 1 kHz, duty cycle at 20% and intensity at 30 mW/cm 2 . The cells were exposed to 30 mW/cm 2 treatment for 20 min everyday and for 13 consecutive days.…”

Section: Lipus Treatmentmentioning

confidence: 99%

Synergies of accelerating differentiation of bone marrow mesenchymal stem cells induced by low intensity pulsed ultrasound, osteogenic and endothelial inductive agent

Jiang

Bao-ru

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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2019) Synergies of accelerating differentiation of bone marrow mesenchymal stem cells induced by low intensity pulsed ultrasound, osteogenic and endothelial inductive agent, Artificial Cells, Nanomedicine, and Biotechnology, 47:1, 673-683, ABSTRACTIn terms to investigate the effect of low-intensity pulsed ultrasound (LIPUS) for differentiation of bone marrow mesenchymal stem cells (BMSCs) and the feasibility of simultaneously inducing into osteoblasts and vascular endothelial cells within the cell culture medium in which two inductive agents are added at the same time with or without LIPUS. Cells were divided into a non-induced group, an osteoblastinduced group, a vascular endothelial-induced group, and a bidirectional differentiation-induced group. Each group was further subdivided into LIPUS and non-LIPUS groups. The cell proliferation in each group was measured by MTT assay. Cell morphological and ultrastructural changes were observed by inverted phase contrast microscopy and transmission electron microscopy. The differentiation of BMSCs was detected by confocal microscopy, flow cytometry and quantitative RT-PCR. Results demonstrated that both osteoblast and vascular endothelial cell differentiation markers were expressed in the bidirectional differentiation induction group and early osteogenesis and angiogenesis appeared. The cell proliferation, differentiation rate and expression of osteocalcin and vWF in the LIPUS groups were all significantly higher than those in the corresponding non-LIPUS group (p < .05), suggesting LIPUS treatment can promote the differentiation efficiency and rate of BMSCs, especially in the bidirectional differentiation induction group. This study suggests the combination of LIPUS and dual-inducing agents could induce and accelerate simultaneous differentiation of BMSCs to osteoblasts and vascular endothelial cells. These findings indicate the method could be applied to research on generating vascularized bone tissue with a shape and function that mimics natural bone to accelerate early osteogenesis and angiogenesis. ARTICLE HISTORY

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Combination of low-intensity pulsed ultrasound and C3H10T1/2 cells promotes bone-defect healing

Abstract: LIPUS treatment effectively promotes C3H10T1/2 cells to enter the growth/split phase from the stationary phase. This process enhances cell proliferation, which consequently promotes bone-defect healing.

Cited by 18 publications

References 15 publications

Low‐intensity pulsed ultrasound activates ERK1/2 and PI3K‐Akt signalling pathways and promotes the proliferation of human amnion‐derived mesenchymal stem cells

Low‐intensity pulsed ultrasound activates ERK1/2 and PI3K‐Akt signalling pathways and promotes the proliferation of human amnion‐derived mesenchymal stem cells

Low‐Intensity Pulsed Ultrasound Relieves Mild and Severe Myelosuppression Induced by Cyclophosphamide in Rabbits

Synergies of accelerating differentiation of bone marrow mesenchymal stem cells induced by low intensity pulsed ultrasound, osteogenic and endothelial inductive agent

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