Low‐intensity pulsed ultrasound activates <scp>ERK</scp>1/2 and <scp>PI</scp>3K‐Akt signalling pathways and promotes the proliferation of human amnion‐derived mesenchymal stem cells

Li, Ling; Wei, Tianqin; He, Lianli; Wang, Yaping; Wang, Yan; Feng, Xiaoxia; Zhang, Wenqian; Xiong, Zhi‐Gang

doi:10.1111/cpr.12383

Cited by 57 publications

(85 citation statements)

References 65 publications

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“…The PI3K/AKt pathway plays an important role in cell growth, survival, proliferation, and cell cycle progression. Previous studies have confirmed that PI3K/AKt has an important role in the proliferation and differentiation of MSCs . Cell cycle progression relies on cyclins and cyclin‐dependent kinase (CDK) .…”

Section: Discussionmentioning

confidence: 99%

Low‐intensity pulsed ultrasound promotes the proliferation of human bone mesenchymal stem cells by activating PI3K/AKt signaling pathways

Xie

Jiang

Wang

et al. 2019

J of Cellular Biochemistry

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Low‐intensity pulsed ultrasound (LIPUS) is a promising therapy that is widely used in clinical applications and fundamental research. Previous research has shown that LIPUS exposure has a positive effect on stem cell proliferation. However, the impact of LIPUS exposure on human bone marrow mesenchymal stem cells (hBMSCs) remains unknown. In our study, the effect and mechanism of LIPUS exposure on the proliferation of hBMSCs were investigated, and the optimal parameters of LIPUS were determined. hBMSCs were obtained and identified by flow cytometry, and the proliferation of hBMSCs was measured using the Cell Counting Kit‐8 assay to determine cell cycle and cell count. Expression levels of the phosphoinositide 3‐kinase (PI3K)/protein kinase B (AKt) pathway proteins and cyclin D1 were determined by western blot analysis. Next, hBMSCs were successfully cultured and identified as multipotent mesenchymal stem cells. We found that LIPUS could promote the proliferation of hBMSCs when the exposure time was 5 or 10 minutes per day. Furthermore, 50 or 60 mW/cm2 LIPUS had a more significant effect on cell proliferation, but if cells were irradiated by LIPUS for 20 minutes once a day, an intensity of at least 50 mW/cm2 could markedly inhibit cell growth. Cell cycle analysis demonstrated that LIPUS treatment drives cells to enter S and G2/M phases from the G0/G1 phase. LIPUS exposure increased phosphorylation of PI3K/AKt and significantly upregulated expression of cyclin D1. However, these effects were inhibited when cells were treated with PI3K inhibitor (LY294002), which in turn reduced LIPUS‐mediated proliferation of hBMSCs. These results suggest that LIPUS exposure may be involved in the proliferation of hBMSCs via activation of the PI3K/AKt signaling pathway and high expression of cyclin D1, and the intensity of 50 or 60 mW/cm2 and exposure time of 5 minutes were determined to be the optimal parameters for LIPUS exposure.

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Section: Discussionmentioning

confidence: 99%

Low‐intensity pulsed ultrasound promotes the proliferation of human bone mesenchymal stem cells by activating PI3K/AKt signaling pathways

Xie

Jiang

Wang

et al. 2019

J of Cellular Biochemistry

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“…The same nanoconstruct was studied to deliver oxygen to hypoxic tissues, exploiting the boiling point of the perfluoropentane core (32°C). In fact, whereas this compound is liquid at room temperature, it is in vapour phase at body temperature, leading [155][156][157][158][159][160][161][162][163][164][165][166][167][168][169][170][171][172] to an higher expansion of the nanobubbles and enhancing the release of loaded oxygen molecules under ultrasound irradiation [102]. In addition to liposomes and polymeric NPs, also metallic and inorganic nanoconstructs could represent efficient platforms for carrying sonosensitizer molecules.…”

Section: Nps Used In the Sonodynamic Therapymentioning

confidence: 99%

“…Chemical Engineering Journal 340 (2018) [155][156][157][158][159][160][161][162][163][164][165][166][167][168][169][170][171][172] residual air bubbles inside the silicon nanoporous structure and by the formation of gas (hydrogen) bubbles due to silicon NPs' dissolution [110]. Nevertheless, concerning the class of NPs employed as nanosensitizers in SDT, the most widely studied are titanium dioxide (TiO 2 ) NPs.…”

Section: G Canavese Et Almentioning

confidence: 99%

“…Alternatively, high-energy shock-waves can be used to minimize the temperature effects while increasing the likelihood of cavitation [93]. Indeed, using single acoustic pulses with a wide frequency range (up to 20 MHz) and high-pressure amplitude (up to 120 MPa) [155][156][157][158][159][160][161][162][163][164][165][166][167][168][169][170][171][172] combined with porphyrins-loaded polymeric NPs, Canaparo et al were able to induce sonodynamic therapy effects on an in-vitro neuroblastoma model [99]. As stated above, the inertial cavitation refers to the rapid growth and violent collapse of bubbles after exposure to ultrasound.…”

Section: Np Interaction With Ultrasonic Wavesmentioning

confidence: 99%

“…The mechanisms involved in the cytotoxic effect are complex and difficult to generalize. In fact, they are strictly dependent on the concentration and physicochemical properties of the specific metal oxides that also affect their G. Canavese et al Chemical Engineering Journal 340 (2018) [155][156][157][158][159][160][161][162][163][164][165][166][167][168][169][170][171][172] behavior in the biological contest [167]. However, from all the proposed cytotoxicity mechanisms by the literature, three of them merit consideration in the application of SDT: (i) the generation of ROS; (ii) the mechanical destruction of cell membranes; (iii) the metal-ion release.…”

Section: Metal Oxide Nps Toxicitymentioning

confidence: 99%

See 2 more Smart Citations

Nanoparticle-assisted ultrasound: A special focus on sonodynamic therapy against cancer

Canavese

Ancona

Racca

et al. 2018

Chemical Engineering Journal

351

247

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• Ultrasound-based therapies are opening new horizons in the oncological field.• Innovative and promising solutions derive from different nanoparticles-assisted ultrasound treatments.• Sonodynamic therapy (SDT) emerged recently as a novel approach for cancer treatment.• Different and complex cell death mechanisms are involved in nanoparticles-assisted SDT.• Nanoparticles-assisted ultrasound is still at its infancy in clinics. A R T I C L E I N F O Keywords:Inertial cavitation Reactive oxygen species Sonoluminescence Sonodynamic Tumor Therapy Cytotoxicity A B S T R A C TAt present, ultrasound radiation is broadly employed in medicine for both diagnostic and therapeutic purposes at various frequencies and intensities. In this review article, we focus on therapeutically-active nanoparticles (NPs) when stimulated by ultrasound. We first introduce the different ultrasound-based therapies with special attention to the techniques involved in the oncological field, then we summarize the different NPs used, ranging from soft materials, like liposomes or micro/nano-bubbles, to metal and metal oxide NPs. We therefore focus on the sonodynamic therapy and on the possible working mechanisms under debate of NPs-assisted sonodynamic treatments. We support the idea that various, complex and synergistics physical-chemical processes take place during acoustic cavitation and NP activation. Different mechanisms are therefore responsible for the final cancer cell death and strongly depends not only on the type and structure of NPs or nanocarriers, but also on the way they interact with the ultrasonic pressure waves. We conclude with a brief overview of the clinical applications of the various ultrasound therapies and the related use of NPs-assisted ultrasound in clinics, showing that this very innovative and promising approach is however still at its infancy in the clinical cancer treatment.

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Active Biomaterials for Bone Tissue Regeneration

Marcotulli,

Iafrate,

Senturk

et al. 2024

Stimuli‐Responsive Materials for Tissue Engineering

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Low‐intensity pulsed ultrasound activates ERK1/2 and PI3K‐Akt signalling pathways and promotes the proliferation of human amnion‐derived mesenchymal stem cells

Abstract: Low-intensity pulsed ultrasound can promote the proliferation of hAD-MSCs, and ERK1/2 and PI3K-Akt signalling pathways may play important roles in this process.

Cited by 57 publications

References 65 publications

Low‐intensity pulsed ultrasound promotes the proliferation of human bone mesenchymal stem cells by activating PI3K/AKt signaling pathways

Low‐intensity pulsed ultrasound promotes the proliferation of human bone mesenchymal stem cells by activating PI3K/AKt signaling pathways

Nanoparticle-assisted ultrasound: A special focus on sonodynamic therapy against cancer

Active Biomaterials for Bone Tissue Regeneration

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