Interface Oral Health Science 2011 2012
DOI: 10.1007/978-4-431-54070-0_60
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Combination of Mutanase and Dextranase Effectively Suppressed Formation of Insoluble Glucan Biofilm by Cariogenic Streptococci

Abstract: Abstract. Cariogenic streptococci produce a-1,3-glucan and a-1,6-glucan, which are the major components of dental biofilms. To investigate the possibility of preventing dental biofilm formation, utility of a combination of mutanase and dextranase was examined in vitro. Experiments: Insoluble glucan biofilms were synthesized by cariogenic streptococci (Streptococcus mutans 10449, S. mutans Ingbritt, Streptococcus sobrinus 6715, and S. sobrinus B13) in the presence of mutanase and/or dextranase in 96-well microp… Show more

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“…It is desirable that the inhibition of glucan synthesis of liposomal nisin be sustain so that a selected formulation would suffice to achieve an efficient preventive effect for oral health. 4,11 Thus, the stable bactericidal activity and long lasting inhibition of glucan synthesis of liposomes will allow us to have expected the dental health. We assessed the data of bactericidal activity and inhibition of glucan synthesis of these encapsulated nisin, therefore we investigated the nisin releasing assay of these liposomes (Figure 3).…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…It is desirable that the inhibition of glucan synthesis of liposomal nisin be sustain so that a selected formulation would suffice to achieve an efficient preventive effect for oral health. 4,11 Thus, the stable bactericidal activity and long lasting inhibition of glucan synthesis of liposomes will allow us to have expected the dental health. We assessed the data of bactericidal activity and inhibition of glucan synthesis of these encapsulated nisin, therefore we investigated the nisin releasing assay of these liposomes (Figure 3).…”
Section: Resultsmentioning
confidence: 99%
“…Solubilized glucans were quantified by the phenol-sulfuric acid method. 11 Inhibition of glucan synthesis was determined according to the amounts of glucan-biofilm in each well compared with those in wells lacking nisin or liposomes.…”
Section: Assay Of Inhibitory Effect Of Liposome-encapsulated Nisin Onmentioning
confidence: 99%
“…Insoluble glucans in each well were washed with 10 mM sodium phosphate buffer (pH 7.0) and dissolved with 200 µl of 1.0 M sodium hydroxide. The amounts of alkaline solubilized glucan were quantified by the phenol-sulfuric acid method [ 15 ]. Inhibition of glucan synthesis was determined according to the amounts of insoluble glucan in each well compared with those in wells lacking nisin or liposomes.…”
Section: Methodsmentioning
confidence: 99%