2008
DOI: 10.1186/1471-2164-9-272
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Combination of native and denaturing PAGE for the detection of protein binding regions in long fragments of genomic DNA

Abstract: BackgroundIn a traditional electrophoresis mobility shift assay (EMSA) a 32P-labeled double-stranded DNA oligonucleotide or a restriction fragment bound to a protein is separated from the unbound DNA by polyacrylamide gel electrophoresis (PAGE) in nondenaturing conditions. An extension of this method uses the large population of fragments derived from long genomic regions (approximately 600 kb) for the identification of fragments containing protein binding regions. With this method, genomic DNA is fragmented b… Show more

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Cited by 3 publications
(1 citation statement)
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“…In contrast, smearing through the lane was observed in the case of the truncated mutant. This demonstrates that the CTR31 protein dissociated from the plasmid during electrophoresis (Tseng et al, 1999;Kaer et al, 2008;Park et al, 2008). Compared with histone H1°, the Topo II CTR required 6-12-fold more protein to produce a shift.…”
Section: Topo Ii Associates With Mitotic Chromosomes Independently Of Nuclear Localization During S Phasementioning
confidence: 99%
“…In contrast, smearing through the lane was observed in the case of the truncated mutant. This demonstrates that the CTR31 protein dissociated from the plasmid during electrophoresis (Tseng et al, 1999;Kaer et al, 2008;Park et al, 2008). Compared with histone H1°, the Topo II CTR required 6-12-fold more protein to produce a shift.…”
Section: Topo Ii Associates With Mitotic Chromosomes Independently Of Nuclear Localization During S Phasementioning
confidence: 99%