Combination of off-line two-dimensional hydrophilic interaction liquid chromatography for polar fraction and two-dimensional hydrophilic interaction liquid chromatography×reversed-phase liquid chromatography for medium-polar fraction in a traditional Chinese medicine

Liang, Zheng; Li, Kuiyong; Wang, Xinliang; Ke, Yanxiong; Jin, Yu; Liang, Xinmiao

doi:10.1016/j.chroma.2011.12.046

Cited by 51 publications

(16 citation statements)

References 20 publications

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“…Recently, a number of attempts have been made to address this problem, for which the application of a double off-line 2D-LC system seems to be an effective approach. For example, in the analysis of components in Scutellaria barbata, a double two-column system has been constructed, including a HILIC/HILIC pattern for the polar fraction analysis and a HILIC/RPLC pattern for the medium-polar fraction analysis [53]. A total of 749 peaks, including numerous peaks corresponding to low-abundance compounds, were detected by this highly orthogonal system.…”

Section: Double Two-column Systemmentioning

confidence: 99%

Application of two-dimensional chromatography in the analysis of Chinese herbal medicines

Cao

Wei

Chen

et al. 2014

Journal of Chromatography A

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Section: Double Two-column Systemmentioning

confidence: 99%

Application of two-dimensional chromatography in the analysis of Chinese herbal medicines

Cao

Wei

Chen

et al. 2014

Journal of Chromatography A

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“…The above examples showed that the separation of complex samples, especially samples from natural product, required a combination of multiform separation techniques. As a mature and effective method, 2D chromatography is perfect for the separation of complex samples and has been widely applied . The orthogonal separation based on different retention mechanisms has remarkable selectivity and peak capacity.…”

Section: Introductionmentioning

confidence: 99%

Purification of lignans from Fructus Arctii using off‐line two‐dimensional supercritical fluid chromatography/reversed‐phase liquid chromatography

Yang

Xin

Wang

et al. 2017

J of Separation Science

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As a common traditional Chinese medicine, Fructus Arctii has important clinical medical values. Its main components are lignans, which are difficult to separate and analyze because of the complex composition, similar chemical structures, and close properties. In this study, an off-line two-dimensional supercritical fluid chromatography/reversed-phase liquid chromatography method, as well as an effective sample pretreatment method based on hydrophilic interaction chromatography material, was developed to enrich the minor lignan fractions and obtain high-purity compounds. In total, 12 high-purity compounds were isolated from Fructus Arctii. Their structures were identified by using high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy, which showed that all were lignans and that most of them were isomers. The results demonstrated the effective off-line two-dimensional supercritical fluid chromatography/reversed-phase liquid chromatography method for the purification of lignans from Fructus Arctii. The separation protocol established here will be beneficial for the separation of complex samples from other kinds of natural products.

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“…The fragment ion was at m/z 269, which was the similar fragmentation pattern of apigenin aglycone. Thus, compound 7 was tentatively identified as apigenin-7-O-glucoside [29].…”

Section: Flavonoid Derivativesmentioning

confidence: 99%

“…In the MS 2 spectra, the aglycone fragment ions corresponded to the luteolin, naringenin, baicalein, scutellarein, and naringenin, which were attributed to the loss of a glucuronic acid moiety (176 Da) from the deprotonated molecule ions. Therefore, these compounds were tentatively identified as luteolin-7-O-glucuronide, naringenin-7-O-glucuronide, baicalin, scutellarin, and naringenin-4′-O-glucuronide [29]. 4′-Hydroxywogonin 13.5 >200 >200…”

Section: Flavonoid Derivativesmentioning

confidence: 99%

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Antileukemic activity of the chemical constituents from Scutellaria barbata D. Don

Ren

Xia

Quan

et al. 2017

Acta Chromatographica

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Summary. Scutellaria barbata D. Don has been used as a traditional Chinese medicine for antitumor and anti-inflammatory. However, there were just a few investigations about S. barbata D. Don according to bioactivity-directed isolation and online identification for the chemical constituents. In this work, eight compounds were isolated from S. barbata D. Don. The three flavonoids indicated the cytotoxic activity against human leukemic Reh cell lines. In addition, the constituents of S. barbata D. Don were further characterized and identified by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC−Q-TOF-MS). The UHPLC− Q-TOF-MS method was in negative ion mode. HPLC separation was performed on a Tosoh TSK gel ODS-100V (4.6 × 150 mm, 3.0 µm) column by gradient elution using water containing 0.3% formic acid and acetonitrile as mobile phase at a flow rate of 0.8 mL min −1 . A total of 18 compounds, including 4 phenolic acids and 14 flavonoids were tentatively characterized and identified by means of the retention time, accurate mass, and characteristic fragment ions.

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Combination of off-line two-dimensional hydrophilic interaction liquid chromatography for polar fraction and two-dimensional hydrophilic interaction liquid chromatography×reversed-phase liquid chromatography for medium-polar fraction in a traditional Chinese medicine

Cited by 51 publications

References 20 publications

Application of two-dimensional chromatography in the analysis of Chinese herbal medicines

Application of two-dimensional chromatography in the analysis of Chinese herbal medicines

Purification of lignans from Fructus Arctii using off‐line two‐dimensional supercritical fluid chromatography/reversed‐phase liquid chromatography

Antileukemic activity of the chemical constituents from Scutellaria barbata D. Don

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