Combination of OipA, BabA, and SabA as candidate biomarkers for predicting Helicobacter pylori-related gastric cancer

Abstract

Helicobacter pylori (H. pylori ) infection is a major cause of chronic gastritis and is highly related to duodenal ulcer (DU) and gastric cancer (GC). To identify H. pylori-related GC biomarkers with high seropositivity in GC patients, differences in levels of protein expression between H. pylori from GC and DU patients were analyzed by isobaric tag for relative and absolute quantitation (iTRAQ). In total, 99 proteins showed increased expression (>1.5-fold) in GC patients compared to DU patients, and 40 of these proteins were categorized by KEGG pathway. The four human disease-related adhesin identified, AlpA, OipA, BabA, and SabA, were potential GC-related antigens, with a higher seropositivity in GC patients (n = 76) than in non-GC patients (n = 100). Discrimination between GC and non-GC patients was improved using multiple antigens, with an odds ratio of 9.16 (95% CI, 2.99–28.07; p < 0.0001) for three antigens recognized. The optimized combination of OipA, BabA, and SabA gave a 77.3% correct prediction rate. A GC-related protein microarray was further developed using these antigens. The combination of OipA, BabA, and SabA showed significant improvement in the diagnostic accuracy and the protein microarray containing above antigens should provide a rapid and convenient diagnosis of H. pylori-associated GC.

Cortactin: A Major Cellular Target of the Gastric Carcinogen Helicobacter pylori

“…These proteins allow the bacteria to adhere onto the surface of epithelial cells. Three major virulence factors act intracellularly by specifically disrupting cell signaling and cell integrity: cytotoxin-associated gene A (CagA), vacuolating cytotoxin A (VacA), and serine protease high temperature requirement A (HtrA) [73][74][75][76]. Virulence depends on presence of the cag pathogenicity island (cagPAI), a genome island that encodes a type IV secretion system (T4SS).…”

Precancerous Gastric Lesions with Helicobacter pylori vacA+/babA2+/oipA+ Genotype Increase the Risk of Gastric Cancer

“…e OipA is one of the porin proteins and proinflammatory proteins associated with severe neutrophil infiltration in IL-8 induction and gastric colonization [15]. It contributes to the pathogenesis and is associated with the elevated risks of PUD and GC [16][17][18]. e cagA gene is located at the end of the cag pathogenicity island (cagPAI), encodes a type IV secretion system (T4SS) that is functional for translocating bacterial effectors into host cytoplasm, and triggers the manipulation of cell signaling pathways and also the induction of the proinflammatory cytokines, specifically interleukin IL-8 [19,20].…”

Association of Helicobacter pylori babA2 gene and gastric cancer risk: a meta-analysis

“…H. pylori strains harboring the cytotoxin-associated antigen (cagA) and the vacuolating toxin A (vacA) have been considered as risk factors for GC [15,17,18]. The OipA, one of porin proteins associated with severe neutrophil infiltration in IL-8 induction and gastric colonization [19], was also associated with GC risk [20][21][22].…”