Combination of PBMC miR-19b-5p, miR-221, miR-25-5p and hypertension correlates with increased risk of heart failure in coronary heart disease patients

Abstract

Objective:The aim of this study was to explore the differences in microRNA (miRNA) profiles in peripheral blood mononuclear cells (PBMCs) between coronary heart disease (CHD) patients with and without heart failure (HF) and to assess the values of differentially expressed miRNAs (DEMs) regarding HF risk in CHD patients.Methods:Six CHD patients with HF and six age- and gender-matched CHD patients without HF were enrolled in the exploration stage, and 44 CHD patients with HF and 42 age- and gender-matched CHD patients without HF were recruited in the validation stage. Peripheral blood samples were collected from all the participants, and PBMCs were separated for miRNA detection. miRNA microarray and quantitative polymerase chain reaction were performed to assess the miRNA expression.Results:In the exploration stage, heat map analysis showed that CHD patients with HF could be distinguished from those without HF using PMBC miRNA expressions; 63 downregulated DEMs and 84 upregulated DEMs in PBMCs were identified in CHD patients with HF using volcano map, and top 8 DEMs were selected based on their p values. In the validation stage, PBMC miR-221, miR-19b-5p, and miR-25-5p were found to be markedly dysregulated in CHD patients with HF. Multiple logistic regression analysis showed PBMC miR-221, miR-19b-5p, miR-25-5p, and hypertension to be the independent predictive factors for HF in CHD patients. A receiver operating characteristic curve demonstrated that area under curve of the combination of miR-221, miR-19b-5p, miR-25-5p, and hypertension was 0.871 (95% CI: 0.794-0.944).Conclusion:CHD patients with and without HF could be differentiated according to PBMC miRNA profiles, and the combination of PBMC miR-19b-5p, miR-221, miR-25-5p, and hypertension correlates with an increased HF risk in CHD patients.

The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma

“…Besides, they have indicated its positive correlation with clinicopathologiacal features and the survival rate of NSCLC patients. Moreover, they have demonstrated that LINK-A promotes proliferation of NSCLC cells 18 , which is consistent with our results. In another study conducted by Liu et al, overexpression of LINK-A in metastatic NSCLC has been reported.…”

“…Considering its crucial role in cancer progression, HIF1α as a transcription factor is involved in several signaling pathways and overlapping molecular mechanisms, each of which could be an encouraging target to be investigated in cancer studies 13 , 14 . In addition to its roles in breast cancer, LINK-A has oncogenic role in ovarian cancer 15 , glioma 16 , and lung cancer 17 , 18 . LINK-A acts as a cytoplasmic scaffold in triple-negative breast cancer cell lines (MDA-MB-231 and MDA-MB-468) leads to the normoxic stabilization of HIF1α 12 .…”

Exosome-Transmitted miR-25 Induced by H. pylori Promotes Vascular Endothelial Cell Injury by Targeting KLF2

“…Moreover, we found that exosomal miR-25 significantly increased the expression of inflammatory factors in vascular endothelial cells. Recently, Yao et al (2018) reported that the expression profile of miR-221, miR-19b-5p, and miR-25-5p in peripheral blood mononuclear cells (PBMCs), and hypertension are the independent predictive factors for heart failure (HF) in CHD patients, and found that the expression of miR-25-5p is significantly increased in PBMCs of CHD patients with HF, suggesting that the increased expression of miR-25-5p is positively correlated with the severity of CHD.…”

Regulation of miRNAs by Natural Antioxidants in Cardiovascular Diseases: Focus on SIRT1 and eNOS

“…Abnormal changes in miRNA expression have been closely linked with the onset and progression of many pathological conditions. They have recently gained popularity as important posttranscriptional modulators of various biological processes such as proliferation, differentiation, apoptosis, stress resistance, and angiogenesis [ 34 , 35 ].…”

“…Since then, many follow-up studies have unraveled the critical role played by miRNAs in the pathophysiology of the cardiovascular system [ 31 , 32 ]. The alteration of miRNA expression and gain- and loss-of-function of miRNAs have been demonstrated in the development of cardiovascular disorders, including cardiac hypertrophy, cardiac fibrosis, cardiomyopathy, aortic stenosis, myocardial infarction, coronary heart disease, and atherosclerosis [ 33 , 34 , 35 , 36 , 37 ]. In this review, we have discussed nine miRNAs whose expression levels are highly related to the regulation of cardiovascular function.…”