Combination of Pre-Operative Radiotherapy and Surgery Suppresses Local Accumulation of Collagen and TGF-β1 in Rats

MMP7 Modulation by Short- and Long-term Radiotherapy in Patients with Rectal Cancer

“…A previous investigation from our group in fact showed that RT and surgery induced depression of TGFβ in rats in the first postoperative week followed by up-regulation in the late period (38,40). Similarly, other authors showed lower levels of active TGFβ in both tumour tissue and rectal mucosa in patients irradiated for rectal cancer (38).…”

Matrilysin Expression Related to Radiation and Microflora Changes in Murine Bowel

“…However, the combination of both radiotherapy regimes and antibiotics led to a short dip in both radiotherapy treatment regimens at 24 h, thereafter significant increases reaching initial levels noted at 48 h compared with antibiotics alone. Earlier studies by our group [28] and Fukuchi et al [29] show that a combination of two therapies (radiotherapy and surgery, and chemotherapy and surgery, respectively) leads to depression of TGF-b in the first postoperative week followed by an up-regulation in the late period. Here antibiotics and surgery lead to a continued dip in TGF-b at 48 h, however, the three therapies here (antibiotics and radiotherapy and surgery) lead to a short lived depression of TGF-b.…”

Evaluation of cellular wound healing using flow cytometry and expanded polytetrafluroethylene implants

“…Cell infiltration of the ePTFE reflects the temporal progression of healing showing an early predominance of leukocytes followed by the influx of macrophages and fibroblasts 3,5 . Frequently reported measures of responses corresponding to tissue repair and evaluated using ePTFE implants include hydroxyproline content, granulation tissue architecture, and cellular infiltration 7–11 . Various cells consistent with wound healing responses have been identified within ePTFE implants using IHC and other staining methods.…”

“…3,5 Frequently reported measures of responses corresponding to tissue repair and evaluated using ePTFE implants include hydroxyproline content, granulation tissue architecture, and cellular infiltration. [7][8][9][10][11] Various cells consistent with wound healing responses have been identified within ePTFE implants using IHC and other staining methods. In studies where ePTFE tubes were removed 7-14 days after insertion, neutrophils, macrophages, lymphocytes, and fibroblasts have been present as well as collagen and new vessels.…”