Combination of quercetin and hyperoside has anticancer effects on renal cancer cells through inhibition of oncogenic microRNA-27a

Abstract

Quercetin and hyperoside (QH) in combination (1:1 ratio) have previously been shown to inhibit the growth of human leukemia cells. Here, we investigated the anticancer activity of the same mixture in 786-O renal cancer cells. QH decreased the generation of reactive oxygen species (ROS) by up to 2.25-fold and increased the antioxidant capacity by up to 3-fold in 786-O cells (3.8-60 μg/ml), whereas IC50 values for viability were 18.2, 18.7 and 11.8 μg/ml, respectively. QH also induced caspase-3 cleavage (2-fold) and increased PARP cleavage. Specificity protein (Sp) transcription factors are overexpressed in cancer cells and regulate genes required for cell proliferation, survival and angiogenesis. QH treatment decreased the expression of Sp1, Sp3 and Sp4 mRNA and this was accompanied by decreased protein expression. Moreover, expression of the Sp-dependent anti-apoptotic survival gene survivin was also significantly reduced, both at the mRNA and protein levels. QH decreased microRNA-27a (miR-27a) and induced the zinc finger protein ZBTB10, an Sp-repressor, suggesting that interactions between QH and the miR-27a-ZBTB10 axis play a role in Sp downregulation. This was confirmed by transfection of cells with a specific mimic for miR-27a, which partially reversed the effects of QH. These findings are consistent with previous studies on botanical anticancer agents in colon cancer cells.

Effect of quercetin glucosides from Allium extracts on HepG2, PC‑3 and HT‑29 cancer cell lines

“…contribute to the variations in the anticancer properties of the extracts. Previous studies revealed the anticancer effects of quercetin glucosides in in vitro studies (15,20,21). The present results indicate that quercetin glucosides are potent in HepG2, HT-29 and PC-3 cancer cell lines.…”

“…Quercetin glucosides are considered to act as antioxidants in the genus Allium (22,23). The combination of quercetin glucosides may contribute to the anti-proliferative activity of onion extracts on cancer cells (20)(21)(22)(23). Among the quercetin glucosides present in onions, 4'-Qmg has been demonstrated to exhibit the most antioxidant activity in cancer cell lines (23).…”

“…The major extractable quercetin glucosides in the Allium cepa L. were 3,4'-Qdg and 4'-Qmg, and 3'-Qmg was also detected in this species. The composition analysis of Allium cepa L is presented in Table I, which indicates that 3,4'-Qdg is the major phenolic compound present in onions (20,21). Certain quercetin glucosides may be cross-linked to cell-wall polysaccharides in the form of dimers that may be attributed to the quercetin glucosides level in the fraction obtained from different genus Allium (20,21).…”

Hyperoside inhibits biofilm formation of Pseudomonas aeruginosa

“…Hyperoside is a flavonol glycoside with variety of biological activities, including antioxidant, anticancer, antihyperglycemic and anti-inflammatory functions (25)(26)(27). However, prior to the current study, hyperoside has demonstrated little antibacterial activity (20,28).…”

Hyperoside induces both autophagy and apoptosis in non-small cell lung cancer cells in vitro

“…Previous studies have shown that hyperoside has anti-oxidant [21] , anticancer [22,23] and anti-inflammatory activities [24][25][26] . Furthermore, hyperoside produced anti-cancer effects through inducing apoptosis [22] and inhibiting of oncogenic microRNA-27a [27] . However, whether hyperoside induces autophagy, and the role it plays in cell death in human lung cancer cells, remain unclear.…”