Combination of site-specific recombination and a conditional selective marker gene allows for the production of marker-free tobacco plants

García-Almodóvar, R.C.; Petri, César; Padilla, Isabel; Burgos, L.

doi:10.1007/s11240-013-0396-x

Search citation statements

Order By: Relevance

Paper Sections

Select...

Ssr Combined With Conditional Genes1

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2016

2021

Publication Types

Select...

Book5

Relationship

Self Cite0

Independent5

Authors

Journals

Cited by 16 publications

(1 citation statement)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An innovative construct (pX6-DAO1), combining the chemical-inducible Cre-LoxP system and the conditional selectable marker gene dao1, was designed to obtain marker-free transgenic tobacco plants (García-Almodovar et al 2014). …”

Section: Ssr Combined With Conditional Genesmentioning

confidence: 99%

Biosafety of Forest Transgenic Trees

Vettori¹,

Gallardo²,

Häggman³

et al. 2016

Forestry Sciences

View full text Add to dashboard Cite

Section: Ssr Combined With Conditional Genesmentioning

confidence: 99%

Biosafety of Forest Transgenic Trees

Vettori¹,

Gallardo²,

Häggman³

et al. 2016

Forestry Sciences

View full text Add to dashboard Cite

Cisgenesis and Intragenesis as New Strategies for Crop Improvement

Moradpour

Abdullah

2017

Crop Improvement

View full text Add to dashboard Cite

Cisgenesis and intragenesis are emerging plant breeding technologies which offer great promise for future acceptance of genetically engineered crops. The techniques employ traditional genetic engineering methods but are confined to transferring of genes and genetic elements between sexually compatible species that can breed naturally. One of the main requirements is the absence of selectable marker genes (such as antibiotic resistance genes) in the genome. Hence the sensitive issues with regard to transfer of foreign genes and antibiotic resistance are overcome. It is a targeted technique involving specific locus; therefore, linkage drag that prolongs the time for crop improvement in traditional breeding does not occur. It has great potential for crop improvement using superior alleles that exist in the untapped germplasm or wild species. Cisgenic and intragenic plants may not face the same stringent regulatory assessment for field release as transgenic plants which is a clear added advantage that would save time. In this chapter, the concepts of cis/intragenesis and the prerequisites for the development of cis/intragenesis plants are elaborated. Strategies for marker gene removal after selection of transformants are discussed based on the few recent reports from various plant species.

show abstract