Combination of Xylanase and Debranching Enzymes Specific to Wheat Arabinoxylan Improve the Growth Performance and Gut Health of Broilers

Lei, Zhao; Shao, Yuxin; Yin, Xiaonan; Yin, Dafei; Guo, Yuming; Yuan, Jianmin

doi:10.1021/acs.jafc.6b01272

Cited by 45 publications

(28 citation statements)

References 43 publications

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“…The inclusion of AXOS encourages beneficial bacteria and protects against pathogenic bacteria [34,35], which consequently enhances gut health. Lei et al documented that dietary Xyl, Abf, and feruloyl esterase improved gut health [36]. Pettey et al also indicated that adding 0.05% β-mannanase and arabinoxylooligosaccharides led to an improved blood IGF-I concentration in growing and finishing pigs [37].…”

Section: Resultsmentioning

confidence: 99%

Effects of Dietary Xylanase and Arabinofuranosidase Combination on the Growth Performance, Lipid Peroxidation, Blood Constituents, and Immune Response of Broilers Fed Low-Energy Diets

Saleh

Kirrella

Abdo

et al. 2019

Animals

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The present study was conducted to examine that impact of dietary xylanase (Xyl) and arabinofuranosidase (Abf) supplementation on the performance, protein and fat digestibility, the lipid peroxidation, the plasma biochemical traits, and the immune response of broilers. A total of 480, un-sexed, and one-day-old broilers (Ross 308) were randomly divided into three treatments with eight replicates, where chicks in the first treatment were fed basal diets and served as the control, chicks in the second treatment were fed diets formulated with reductions of 90 kcal/kg, and chicks in the third treatment were fed the same formulated diets used in the second group as well as the Xyl and Abf combination (Rovabio® Advance). Feed intake was decreased by the low energy diet, leading to an enhancement in feed efficiency enzyme supplementation in the low energy diet (p < 0.015). Both protein and fat digestibility were improved (p < 0.047) due to enzyme supplementation. Moreover, enzyme supplementation increased muscle total lipids content and decreased muscle thiobarbituric acid retroactive substance content. Furthermore, diets supplemented with Xyl and Abf exhibited an increase in antibody titers against the Newcastle disease virus (p < 0.026). In addition, enzyme supplementation increased gene expression related to growth and gene expression related to fatty acid synthesis. It could be concluded that dietary Xyl and Abf supplementation had beneficial impacts on growth, nutrient digestibility, lipid peroxidation, immune response, and gene expressions related to growth and fatty acid synthesis in broiler chickens fed low-energy diets.

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Section: Resultsmentioning

confidence: 99%

Effects of Dietary Xylanase and Arabinofuranosidase Combination on the Growth Performance, Lipid Peroxidation, Blood Constituents, and Immune Response of Broilers Fed Low-Energy Diets

Saleh

Kirrella

Abdo

et al. 2019

Animals

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“…comprising 15–35% of each plant’s dry weight 1 . Xylan is a heterogeneous polysaccharide consisting of a backbone of β-1,4-xylose units that are attached by side chains such as arabinose, methyl glucuronic acid, and acetate groups 2 . A series of enzymes is essential when xylan is completely degraded to monosaccharide, mainly including endo-β-xylanases (EC 3.2.1.8) and β-D-xylosidases (EC 3.2.1.37).…”

Section: Introductionmentioning

confidence: 99%

Production and Characteristics of a Novel Xylose- and Alkali-tolerant GH 43 β-xylosidase from Penicillium oxalicum for Promoting Hemicellulose Degradation

Zhao

2017

Sci Rep

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β-xylosidase is a pivotal enzyme for complete degradation of xylan in hemicelluloses of lignocelluloses, and the xylose- and alkali-tolerant β-xylosidase with high catalytic activity is very attractive for promoting enzymatic hydrolysis of alkaline-pretreated lignocellulose. In this study, a novel intracellular glycoside hydrolase family 43 β-xylosidase gene (xyl43) from Penicillium oxalicum 114-2 was successfully high-level overexpressed in Pichia pastoris, and the secreted enzyme was characterized. The β-xylosidase Xyl43 exhibited great pH stability and high catalytic activity in the range of pH 6.0 to 8.0, and high tolerance to xylose with the Ki value of 28.09 mM. The Xyl43 could effectively promote enzymatic degradation of different source of xylan and hemicellulose contained in alkaline-pretreated corn stover, and high conversion of xylan to xylose could be obtained.

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“…This xylanase has potential utility in animal feed to improve nutrient digestibility and growth performance for animals, especially broiler chickens and hens [53]. …”

Section: Discussionmentioning

confidence: 99%

Gene Expression and Molecular Characterization of a Xylanase from Chicken Cecum Metagenome

Al-Darkazali

Meevootisom

Isarangkul

et al. 2017

International Journal of Microbiology

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A xylanase gene xynAMG1 with a 1,116-bp open reading frame, encoding an endo-β-1,4-xylanase, was cloned from a chicken cecum metagenome. The translated XynAMG1 protein consisted of 372 amino acids including a putative signal peptide of 23 amino acids. The calculated molecular mass of the mature XynAMG1 was 40,013 Da, with a theoretical pI value of 5.76. The amino acid sequence of XynAMG1 showed 59% identity to endo-β-1,4-xylanase from Prevotella bryantii and Prevotella ruminicola and 58% identity to that from Prevotella copri. XynAMG1 has two conserved motifs, DVVNE and TEXD, containing two active site glutamates and an invariant asparagine, characteristic of GH10 family xylanase. The xynAMG1 gene without signal peptide sequence was cloned and fused with thioredoxin protein (Trx.Tag) in pET-32a plasmid and overexpressed in Escherichia coli Tuner™(DE3)pLysS. The purified mature XynAMG1 was highly salt-tolerant and stable and displayed higher than 96% of its catalytic activity in the reaction containing 1 to 4 M NaCl. It was only slightly affected by common organic solvents added in aqueous solution to up to 5 M. This chicken cecum metagenome-derived xylanase has potential applications in animal feed additives and industrial enzymatic processes requiring exposure to high concentrations of salt and organic solvents.

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Combination of Xylanase and Debranching Enzymes Specific to Wheat Arabinoxylan Improve the Growth Performance and Gut Health of Broilers

Cited by 45 publications

References 43 publications

Effects of Dietary Xylanase and Arabinofuranosidase Combination on the Growth Performance, Lipid Peroxidation, Blood Constituents, and Immune Response of Broilers Fed Low-Energy Diets

Effects of Dietary Xylanase and Arabinofuranosidase Combination on the Growth Performance, Lipid Peroxidation, Blood Constituents, and Immune Response of Broilers Fed Low-Energy Diets

Production and Characteristics of a Novel Xylose- and Alkali-tolerant GH 43 β-xylosidase from Penicillium oxalicum for Promoting Hemicellulose Degradation

Gene Expression and Molecular Characterization of a Xylanase from Chicken Cecum Metagenome

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