Combinatorial approach to the selection of active microorganisms in biotransformation: Application to sinomenine

Anticipating the fate and impact of organic environmental contaminants: A new approach applied to the pharmaceutical furosemide

“…To obtain the biotic TPs of furosemide, a selection of microorganisms among seventytwo strains of bacteria and fungi was performed. To complete the screening using minimal time and assays, we used our recently developed combinatorial approach (Fromentin et al, 2012;Joyeau et al, 2013). This method is based on incubating the substrate in the presence of five microorganisms (three fungi and two bacteria) in a reaction buffer.…”

The present and future synthetic strategies of structural modifications of sinomenine

“…In addition, the biotransformation of 4-O-protected derivatives at the D-ring was reported using two screened microorganisms, Mucor plumbeus and Absidia corymbifera (Scheme 42). 80 The reaction of 95 with M. plumbeus afforded N-demethylated derivative 99 (31%) and N-oxide derivative 113 (24%). Both derivatives were the metabolites which resulted from a detoxifying action of microorganisms.…”

Combinatory Library of Microorganisms in the Selection of Reductive Activity Applied to a Ketone Mixture: Unexpected Highlighting of an Enantioselective Oxidative Activity

“…Under these conditions, the active yeast was selected by performing only four incubations instead of the twelve needed in the traditional approach [ 12 ]. This approach was used to test 24 microorganisms (16 fungi and 8 bacteria) on their ability to transform two sinomenine derivatives, and it allowed us to select active microorganisms by performing 8 incubations for one and 10 for the other derivative [ 13 ]. Since then, other studies have been successfully carried out with this approach: one concerns the fate of furosemide in the environment [ 14 ], where four active strains were selected among seventy-two in only eight assays, and the other is about the search for molecular diversity from ilimaquinone, a molecule with various biological activities [ 15 ].…”