Combinatorial Biobleaching of Mixedwood Pulp with Lignolytic and Hemicellulolytic Enzymes for Paper Making

Angural, Steffy; Rana, Monika; Sharma, Alisha; Warmoota, Rahul; Puri, Neena; Gupta, Naveen

doi:10.1007/s12088-020-00867-6

Cited by 25 publications

(7 citation statements)

References 20 publications

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“…The phenolics compounds exhibit low solubility in water and require a solvent system in a few cases. Laccase shows a wide range of specificity towards phenolics substrate [17,50]. After incubation in various solvents, RvLac exhibited a significant decline in the residual activity to the ranges of 8.9-68.7% with the order propanol [ ethanol [ methanol [ benzene [ acetone (Fig.…”

Section: Solvent Stability and Bisphenol A Degradationmentioning

confidence: 95%

Rhus vernicifera Laccase Immobilization on Magnetic Nanoparticles to Improve Stability and Its Potential Application in Bisphenol A Degradation

et al. 2020

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In the present study, Rhus vernicifera laccase (RvLac) was immobilized through covalent methods on the magnetic nanoparticles. Fe 2 O 3 and Fe 3 O 4 nanoparticles activated by 3-aminopropyltriethoxysilane followed with glutaraldehyde showed maximum immobilization yields and relative activity up to 81.4 and 84.3% at optimum incubation and pH of 18 h and 5.8, respectively. The maximum RvLac loading of 156 mg/g of support was recorded on Fe 2 O 3 nanoparticles. A higher optimum pH and temperature of 4.0 and 45 °C were noted for immobilized enzyme compared to values of 3.5 and 40 °C for free form, respectively. Immobilized RvLac exhibited better relative activity profiles at various pH and temperature ranges. The immobilized enzyme showed up to 16-fold improvement in the thermal stability, when incubated at 60 °C, and retained up to 82.9% of residual activity after ten cycles of reuses. Immobilized RvLac exhibited up to 1.9-fold higher bisphenol A degradation efficiency potential over free enzyme. Previous reports have demonstrated the immobilization of RvLac on nonmagnetic supports. This study has demonstrated that immobilization of RvLac on magnetic nanoparticles is very efficient especially for achieving high loading, better pH and temperature profiles, and thermal-and solvents-stability, high reusability, and higher degradation of bisphenol A.

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Section: Solvent Stability and Bisphenol A Degradationmentioning

confidence: 95%

Rhus vernicifera Laccase Immobilization on Magnetic Nanoparticles to Improve Stability and Its Potential Application in Bisphenol A Degradation

et al. 2020

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“…Clarke et al [46] has also reported the use of a mixture of xylanase and mannanase/s but the enzymes were produced by growing the organisms individually and for pulp biobleaching enzymes were added in a sequential manner. Gupta et al [18] and Angural et al [47,48] have also shown the pulp biobleaching using a mixture of lignolytic and hemicellulolytic enzymes.…”

Section: R (Kappa Number Reductionmentioning

confidence: 98%

Bleach enhancement of mixed wood pulp by mixture of thermo-alkali-stable xylanase and mannanase derived through co-culturing of Alkalophilic Bacillus sp. NG-27 and Bacillus nealsonii PN-11

Angural

Bala

Kumar

et al. 2021

Heliyon

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The Application of a combination of enzymes is the best alternative to reduce the use of chemicals in the paper industry. Bacillus sp. NG-27 and Bacillus nealsonii PN-11 are known to produce thermoalkali stable xylanse (X) and mannanase (M) respectively having potential for pulp biobleaching. The Present study, reports the production of a mixture of X þ M by co-culturing of strains in SSF and standardizing its application for pulp biobleaching. Production of enzymes by co-cultivation in SSF was optimized by statistical methods. Substantial increase in the yield of enzymes; 3.61 fold of xylanase and 37.71 fold of mannanase was achieved. Application of enzyme cocktail for pulp biobleaching resulted in a 45.64% reduction of kappa number with 55 IU g À1 odp of enzyme dose (xylanase:mannanase; 3:1) at pH 8.0 in 1h at 65 C along with significant increase in brightness (11%) and whiteness (75%). The Same quality of paper as made up from chemical treated pulp can be made from enzyme-treated pulp with 30% less use of chlorine. Structural analysis of enzyme-treated pulp showed dissolution of hemicellulose as indicated by pores, cracks and increased roughness all over the surface. Cocktail of X þ M produced economically in a single fermentation having all the requisite characteristics for pulp biobleaching is a highly suitable candidate for application in the pulp and paper industry.

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“…Speci c examples include the xylanase produced by alkaliphilic Actinomycetes, which has high enzymatic activity and thermal stability (Ahlawat et al 2007). When it was used in the paper industry, the quality of paper could be improved (Jagannathan et al 2021;Angural et al 2020). In addition, alkaline enzymes produced by alkaliphilic Actinomycetes could also be used in sewage treatment, food, textiles, and other areas (Mitsuiki et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Characterization and identification of filamentous bacterium XJ-16 and analysis of its bioactive components

Jiang

Bai

Luo

et al. 2023

Preprint

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Actinomycetes, which can produce a variety of bioactive compounds in the metabolic process, is one of the important sources of novel drugs, enzymes, anti-tumor drugs and enzyme inhibitors. It has been the focus of researchers to find and develop Actinomycetes with special characters. Strain XJ-16 is a blue alkali-resistant filamentous bacterium with high antimicrobial activity isolated from saline-alkali land of Xinjiang. Based on the classification, the enzyme production, metabolite antibacterial activity, and antibacterial substance isolation of XJ-16 were explored. which showed that XJ-16 belongs to the blue group of Streptomyces sp, and it can secrete cellulase, lipase, urease, protease, catalase and oxidase during metabolism. In addition, the bacteriostatic substance secreted by the strain XJ-16 showed inhibitory effects against both Gram-positive and Gram-negative bacteria, as well as the yeast Candida albicans. Then it was found that the bacteriostasis produced by XJ-16 has strong tolerance to acid, weak tolerance to alkali, and easy to be inactivated. After tested by HPLC, the retention time of antimicrobial substance was 13.261 min. This study provides a theoretical basis for the search for new antibacterial compounds and the further development of blue alkaline Actinomycetes XJ-16.

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Combinatorial Biobleaching of Mixedwood Pulp with Lignolytic and Hemicellulolytic Enzymes for Paper Making

Cited by 25 publications

References 20 publications

Rhus vernicifera Laccase Immobilization on Magnetic Nanoparticles to Improve Stability and Its Potential Application in Bisphenol A Degradation

Rhus vernicifera Laccase Immobilization on Magnetic Nanoparticles to Improve Stability and Its Potential Application in Bisphenol A Degradation

Bleach enhancement of mixed wood pulp by mixture of thermo-alkali-stable xylanase and mannanase derived through co-culturing of Alkalophilic Bacillus sp. NG-27 and Bacillus nealsonii PN-11

Characterization and identification of filamentous bacterium XJ-16 and analysis of its bioactive components

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