Combinatorial biophysical cue sensor array for controlling neural stem cell fate

Lee, Jong Min; Kang, Woon Sang; Lee, Kyoung G.; Cho, Hyeon-Yeol; Conley, Brian; Ahrberg, Chrisitian D.; Lim, Jae Hyun; Mo, Sung Joon; Mun, Seok Gyu; Kim, Eun Joong; Choi, Jeong Woo; Lee, Ki‐Bum; Lee, Seok Jae; Chung, Bong Geun

doi:10.1016/j.bios.2020.112125

Cited by 21 publications

(25 citation statements)

References 53 publications

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“…Since the physical mixing is proceeded by pipetting, AgNW is randomly distributed in a 3D conductive blended hydrogel. In the previous study, it was conducted to mix AgNWs and reduced graphene oxide (rGO) to generate a blended hydrogel for increasing the electrical conductivity [24]. Although rGO and AgNWs were randomly distributed in a 3D hydrogel that was similar to our study, the neural stem cells were successfully differentiated into nerve cells through electrical stimulation.…”

Section: Analysis Of Conductive Gelma-collagen-agnw Blended Hydrogel Propertiesmentioning

confidence: 67%

“…To improve the electrical conductivity and mechanical strength, AgNW was added to the GelMA-collagen hydrogel and this gelation condition was also optimized. The ratio of AgNW was fixed at 10% v/v for the total volume of the GelMA-collagen hydrogel, as previously described [24,25]. The ratios of GelMA, collagen, and AgNW were mixed in ratios of 5:5:1, 7:3:1, and 9:1:1 (Supplementary Material, Figure S2).…”

Section: Optimization Of Conductive Gelma-collagen-agnw Blended Hydrogel Conditionsmentioning

confidence: 99%

“…The silver nanowire (AgNW), showing excellent conductivity and flexibility, has been widely used as an electrode [20], conductive film [21], and energy harvesting material [22]. The hydrogel containing the AgNW was also employed for flexible skin [23], cell differentiation [24,25], biosensor [26], and actuator [27]. The conductive hydrogel has been previously generated by using collagen and AgNW [28].…”

Section: Introductionmentioning

confidence: 99%

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Conductive GelMA–Collagen–AgNW Blended Hydrogel for Smart Actuator

Lim

Cho

et al. 2021

Polymers

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Blended hydrogels play an important role in enhancing the properties (e.g., mechanical properties and conductivity) of hydrogels. In this study, we generated a conductive blended hydrogel, which was achieved by mixing gelatin methacrylate (GelMA) with collagen, and silver nanowire (AgNW). The ratio of GelMA, collagen and AgNW was optimized and was subsequently gelated by ultraviolet light (UV) and heat. The scanning electron microscope (SEM) image of the conductive blended hydrogels showed that collagen and AgNW were present in the GelMA hydrogel. Additionally, rheological analysis indicated that the mechanical properties of the conductive GelMA–collagen–AgNW blended hydrogels improved. Biocompatibility analysis confirmed that the human umbilical vein endothelial cells (HUVECs) encapsulated within the three-dimensional (3D), conductive blended hydrogels were highly viable. Furthermore, we confirmed that the molecule in the conductive blended hydrogel was released by electrical stimuli-mediated structural deformation. Therefore, this conductive GelMA–collagen–AgNW blended hydrogel could be potentially used as a smart actuator for drug delivery applications.

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Section: Analysis Of Conductive Gelma-collagen-agnw Blended Hydrogel Propertiesmentioning

confidence: 67%

Section: Optimization Of Conductive Gelma-collagen-agnw Blended Hydrogel Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Conductive GelMA–Collagen–AgNW Blended Hydrogel for Smart Actuator

Lim

Cho

et al. 2021

Polymers

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“… 207 In addition to groove structures, nanopillars were also found to be promising to enhance neural stem cell differentiation and regulate neurite outgrowths. 208 In addition to neuronal differentiation, micro- and nanogroove structures have been shown to promote osteogenic differentiation, 209,210 myogenesis and myotube alignment, 211 and chondrogenic differentiation. 212 …”

Section: Surface Topography Affect Cell Responses On Hydrogelmentioning

confidence: 99%

The effects of surface topography modification on hydrogel properties

2021

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Hydrogel has been an attractive biomaterial for tissue engineering, drug delivery, wound healing, and contact lens materials, due to its outstanding properties, including high water content, transparency, biocompatibility, tissue mechanical matching, and low toxicity. As hydrogel commonly possesses high surface hydrophilicity, chemical modifications have been applied to achieve the optimal surface properties to improve the performance of hydrogels for specific applications. Ideally, the effects of surface modifications would be stable, and the modification would not affect the inherent hydrogel properties. In recent years, a new type of surface modification has been discovered to be able to alter hydrogel properties by physically patterning the hydrogel surfaces with topographies. Such physical patterning methods can also affect hydrogel surface chemical properties, such as protein adsorption, microbial adhesion, and cell response. This review will first summarize the works on developing hydrogel surface patterning methods. The influence of surface topography on interfacial energy and the subsequent effects on protein adsorption, microbial, and cell interactions with patterned hydrogel, with specific examples in biomedical applications, will be discussed. Finally, current problems and future challenges on topographical modification of hydrogels will also be discussed.

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“…Combining more than two cues can potentially have a greater effect on NSCs modulation. Lee et al explored this possibility through a multi biophysical cues array capable of directing NSCs fate [41]. To produce both a topographical and a mechanical cue, PUNO a mixture of polyurethane and Norland optical adhesive 61 was patterned into a flexible Nano pillar array (Fig.…”

Section: Topography-guided Stem Cell Differentiationmentioning

confidence: 99%

Recent Developments in Surface Topography-Modulated Neurogenesis

Amri¹,

Kim

Lee

2021

BioChip J

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Repairing a nervous system damaged following an injury or as a consequence of neurodegeneration is still a challenging task. Yet, topography-based tissue engineering of neurons from different cell sources has demonstrated promising potential in the field. Substrate patterning through a range of methods including photolithography, nanofibers electrospinning, or microimprinting can not only impact the maturity of these vital neural cells but can also orient the differentiation of neural stem cells, a more prolific source of neurons. Furthermore, it has been shown that topography can guide the trans-differentiation of mesenchymal stem cells, a readily accessible cell source, towards a neuronal fate. Thus, in this review, we will cover the most recent methods used in topography-based neural tissue engineering.

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Combinatorial biophysical cue sensor array for controlling neural stem cell fate

Cited by 21 publications

References 53 publications

Conductive GelMA–Collagen–AgNW Blended Hydrogel for Smart Actuator

Conductive GelMA–Collagen–AgNW Blended Hydrogel for Smart Actuator

The effects of surface topography modification on hydrogel properties

Recent Developments in Surface Topography-Modulated Neurogenesis

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