Combinatorial effect of Gallic acid and Catechin on some biochemical and pro-inflammatory markers in CCl4-mediated hepatic damage in rats

Oriakhi, Kelly; Orumwensodia, Kissinger

doi:10.1016/j.phyplu.2020.100017

Cited by 8 publications

(1 citation statement)

References 21 publications

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“…The decreased Tlr9 expression in PPW-treated cells was due to the reduction of mtDNA leakage. Accumulated evidence shows that catechin [ 50 ], gallic acid [ 51 ], and quercetin [ 52 ] facilitate mitochondrial stress via reduced ROS production by enhancing catalase expression. Moreover, these polyphenols were also found to inhibit cytokine-encoding genes, including IL-6, TNF-α, and IL-1β by suppressing TLR9/MyD88/NF-κB-dependent cytokines signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Effects of Pogonatherum paniceum (Lamk) Hack extract on anti-mitochondrial DNA mediated inflammation by attenuating Tlr9 expression in LPS-induced macrophages

Thongboontho,

Petcharat,

Munkong

et al. 2023

Nutr Res Pract

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BACKGROUND/OBJECTIVES Mitochondrial DNA leakage leads to inflammatory responses via endosome activation. This study aims to evaluate whether the perennial grass water extract ( Pogonatherum panicum ) ameliorate mitochondrial DNA (mtDNA) leakage. MATERIALS/METHODS The major bioactive constituents of P. paniceum (PPW) were investigated by high-performance liquid chromatography, after which their antioxidant activities were assessed. In addition, RAW 264.7 macrophages were stimulated with lipopolysaccharide, resulting in mitochondrial damage. Quantitative polymerase chain reaction and enzyme-linked immunosorbent assay were used to examine the gene expression and cytokines. RESULTS Our results showed that PPW extract-treated activated cells significantly decrease reactive oxygen species and nitric oxide levels by reducing the p22 phox and iNOS expression and lowering cytokine-encoding genes, including IL-6 , TNF-α , IL-1β , PG-E2 and IFN-γ relative to the lipopolysaccharide (LPS)-activated macrophages. Furthermore, we observed that LPS enhanced the mtDNA leaked into the cytoplasm, increasing the transcription of Tlr9 and signaling both MyD88 / Irf7- dependent interferon and MyD88/NF-κb p65-dependent inflammatory cytokine mRNA expression but which was alleviated in the presence of PPW extract. CONCLUSIONS Our data show that PPW extract has antioxidant and anti-inflammatory activities by facilitating mtDNA leakage and lowering the Tlr9 expression and signaling activation.

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Section: Discussionmentioning

confidence: 99%