2015
DOI: 10.1163/15685411-00002859
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Combinatorial in vitro RNAi of two neuropeptide genes and a pharyngeal gland gene on Meloidogyne incognita

Abstract: Root-knot nematodes are the most economically important group of plant-parasitic nematodes. In the present study, functional validation using in vitro RNAi was carried out on Meloidogyne incognita with two FMRFamide-like peptide genes, flp-14 and flp-18, and a subventral pharyngeal gland specific gene, 16D10. It was found that RNAi silencing of each gene reduced the attraction of M. incognita at different time intervals both in combination and individually. Silencing of the genes reduced nematode infection by … Show more

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Cited by 15 publications
(12 citation statements)
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References 38 publications
(46 reference statements)
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“…Hence, it is possible that Mi-flp1 could have similar functional role in M. incognita. Previously, we had reported the expression site of Mi-flp12 and Mi-flp18 in the nerve cells, involved in the various biological and physiological process of M. incognita J2s (Banakar et al, 2015;Banakar and Rao, 2016). These findings support the involvement of the three FLPs in locomotion, host finding, reproduction and other physiological roles in the nematodes (White et al, 1986;Kimber et al, 2002Kimber et al, , 2007Rogers et al, 2003;Johnston et al, 2010;Kumari et al, 2017).…”
Section: Discussionmentioning
confidence: 99%
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“…Hence, it is possible that Mi-flp1 could have similar functional role in M. incognita. Previously, we had reported the expression site of Mi-flp12 and Mi-flp18 in the nerve cells, involved in the various biological and physiological process of M. incognita J2s (Banakar et al, 2015;Banakar and Rao, 2016). These findings support the involvement of the three FLPs in locomotion, host finding, reproduction and other physiological roles in the nematodes (White et al, 1986;Kimber et al, 2002Kimber et al, , 2007Rogers et al, 2003;Johnston et al, 2010;Kumari et al, 2017).…”
Section: Discussionmentioning
confidence: 99%
“…RNAi has been the mainstay tool for functional validation of genes in phytonematodes (Lilley et al, 2007;Dutta et al, 2015a,b). However, reports of gene silencing to explore the function of FLPergic molecules in plant nematodes are finite (Kimber et al, 2007;Dalzell et al, 2010;Papolu et al, 2013;Dong et al, 2014;Banakar et al, 2015). Here, we disrupted three individual FLPs and their combinatorial fusion gene through in vitro RNAi (dsRNA soaking) in M. incognita J2s which resulted in perturbed the expression of target transcripts of each of the FLPs.…”
Section: Discussionmentioning
confidence: 99%
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