Combined Analytical Approaches to Standardize and Characterize Biomaterials Formulations: Application to Chitosan-Gelatin Cross-Linked Hydrogels

Magli, Sofia; Rossi, Lorenzo; Consentino, Cesare; Bertini, Sabrina; Nicotra, Francesco; Russo, Laura

doi:10.3390/biom11050683

Cited by 12 publications

(5 citation statements)

References 64 publications

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“…A multi-technique characterization of Ch derivatives was conducted, to measure physico-chemical properties (i.e., steric hindrance, charge, viscosity) and subsequently correlate them to the effects observed in hTf glycoforms electrophoretic separation, where the CH derivatives are used as dynamic coatings. Figure 3 illustrates the results from NMR analysis, which is one of the major methods used for determining the DA for chitosan [41] and DS for its derivatives [42,43]. In Table 1, the chemical shift values were reported.…”

Section: Resultsmentioning

confidence: 99%

Chitosan derivatives as dynamic coatings for transferrin glycoform separation in capillary electrophoresis

Porpiglia,

Tagliaro,

Pellegrini

et al. 2024

International Journal of Biological Macromolecules

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Section: Resultsmentioning

confidence: 99%

Chitosan derivatives as dynamic coatings for transferrin glycoform separation in capillary electrophoresis

Porpiglia,

Tagliaro,

Pellegrini

et al. 2024

International Journal of Biological Macromolecules

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“…Thermogravimetric analysis is an effective method to detect the thermal stability of scaffolds before and after cross-linking, 40 the thermal stability results of decellularized scaffolds are shown in Figure 5. DSC-TGA was used to detect the thermostability of decellularized scaffolds before and after crosslinking.…”

Section: Resultsmentioning

confidence: 99%

Construction and properties detection of 3D micro-structure scaffolds base on decellularized sheep kidney before and after crosslinking

Zheng

Yang

et al. 2023

J Biomater Appl

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Decellularized extracellular matrix is one form of natural material in tissue engineering. The process of dECM retains the tissue microstructure, provides good cell adhesion sites, maintains most of biological signals that promotes the survival and differentiation ability of cells. In this study, sheep kidney was decellularized followed by histochemical staining, elemental analysis and scanning electron microscopy characterizations. The dECM scaffold was prepared with different sequences of freeze drying technology, crosslinking and the water absorption, porosity, mechanical strength with subsequent thermogravimetric analysis, Infrared spectroscopy and biocompatibility tests. Our results indicated that these decellularized treatments of sheep kidney can effectively remove DNA and retain uniform pore size distribution. After crosslinking the scaffold’s water absorption decreased from 987.56 ± 40.21% to 934.39 ± 39.61%, the porosity decreased from 89.64 ± 3.2% to 85.09 ± 17.63%, and the compression modulus increased from 304.32 ± 25.43 kPa to 459.53 ± 38.92 kPa, with thermal process the percentage of weight loss decreased from 66.57% to 44.731%, in addition, the composition didn’t change significantly, crosslinking could also promote the stability. In terms of biocompatibility, the number of viable cells increased significantly with the days. In conclusion, the crosslinked decellularized sheep kidney extracellular matrix scaffold reduced water absorption and porosity slightly, but has a significant increase in mechanical properties, and presented excellent biocompatibility which are beneficial to cell adhesion, growth and differentiation.

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“…It combines the hydrogel properties of natural substances and electrostatic interactions to achieve adhesion in the oral mucosa [ 113 ]. Gel and CS cross-linked hydrogels can maintain a stable extracellular matrix-like structure for a long time [ 157 ]. A natural polysaccharide-based hydrogel combining CS and agarose holds promise for biomimetic remineralization of human tooth enamel [ 158 ].…”

Section: Other Techniques In the Treatment Of Oral Ulcersmentioning

confidence: 99%

Advances in the Application of Electrospun Drug-Loaded Nanofibers in the Treatment of Oral Ulcers

et al. 2022

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Oral ulcers affect oral and systemic health and have high prevalence in the population. There are significant individual differences in the etiology and extent of the disease among patients. In the treatment of oral ulcers, nanofiber films can control the drug-release rate and enable long-term local administration. Compared to other drug-delivery methods, nanofiber films avoid the disadvantages of frequent administration and certain side effects. Electrospinning is a simple and effective method for preparing nanofiber films. Currently, electrospinning technology has made significant breakthroughs in energy-saving and large-scale production. This paper summarizes the polymers that enable oral mucosal adhesion and the active pharmaceutical ingredients used for oral ulcers. Moreover, the therapeutic effects of currently available electrospun nanofiber films on oral ulcers in animal experiments and clinical trials are investigated. In addition, solvent casting and cross-linking methods can be used in conjunction with electrospinning techniques. Based on the literature, more administration systems with different polymers and loading components can be inspired. These administration systems are expected to have synergistic effects and achieve better therapeutic effects. This not only provides new possibilities for drug-loaded nanofibers but also brings new hope for the treatment of oral ulcers.

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Combined Analytical Approaches to Standardize and Characterize Biomaterials Formulations: Application to Chitosan-Gelatin Cross-Linked Hydrogels

Cited by 12 publications

References 64 publications

Chitosan derivatives as dynamic coatings for transferrin glycoform separation in capillary electrophoresis

Chitosan derivatives as dynamic coatings for transferrin glycoform separation in capillary electrophoresis

Construction and properties detection of 3D micro-structure scaffolds base on decellularized sheep kidney before and after crosslinking

Advances in the Application of Electrospun Drug-Loaded Nanofibers in the Treatment of Oral Ulcers

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