Combined bioderivatization and engineering approach to improve the efficiency of geraniol production

Abstract

Constructing microbial factories offers a sustainable strategy for terpenoid synthesis; however, the accumulation of terpenoid products is often toxic to cells and hampers production yields. Here, we developed a bioderivatization...

Two-Phase Fermentation Systems for Microbial Production of Plant-Derived Terpenes

“…By overexpressing the synthase and heterologous MVA pathway in E. coli and subsequently employing fed-batch fermentation with isopropyl myristate as the organic phase, the production of geraniol was significantly enhanced, resulting in a yield of 2 g/L compared to the initial 78.8 mg/L obtained after basic fermentation in a bioreactor [ 65 ]. In another study, by adding the same SP in fed-batch fermentation, the production of geraniol was further increased to 13.19 g/L [ 80 ]. Different organic solvents, such as n-decane [ 81 ] and n-dodecane [ 82 ], had been employed as the SP in the TPF, which also increased the production of geraniol.…”

Recent Advances and Multiple Strategies of Monoterpenoid Overproduction in Saccharomyces cerevisiae and Yarrowia lipolytica

“…Wang et al introduced a bioderivatization strategy, combined with combined the hydrolysis of geranyl acetate and solid phase adsorption, which led to an impressive yield of 13.19 g/L of geraniol. 110 In a separate study, Zheng et al employed computer-assisted single-point and activity data-driven multipoint saturation mutant libraries, resulting in an extraordinary yield of 291.3 g/L/day. 111 Significantly, the most effective mutant obtained with this technique might be employed for commercial one-step geraniol biosynthesis.…”

“…However, it is worth noting that the highest yield of geraniol has been achieved in E. coli . Wang et al introduced a bioderivatization strategy, combined with combined the hydrolysis of geranyl acetate and solid phase adsorption, which led to an impressive yield of 13.19 g/L of geraniol . In a separate study, Zheng et al employed computer-assisted single-point and activity data-driven multipoint saturation mutant libraries, resulting in an extraordinary yield of 291.3 g/L/day .…”

Microaerobic Fermentation Enables High-Titer Biosynthesis of the Rose Monoterpenes Geraniol and Geranyl Acetate in Escherichia coli

“…Particularly, its demand in the clothing goods, alcoholic beverages, and frozen foods is growing rapidly. Apart from the perfume and cosmetic industry, geraniol is also applied in pest control (e.g., mosquito repellent and cockroach insecticides with low mammalian toxicity) and pharmaceutical industries, − such as serving as the key precursor for anticancer drugs like monoterpene indole alkaloids (MIAs). , Geranyl acetate, the ester derivative of geraniol, is also widely used in the cosmetic industry due to its floral and fruity scent and can also be easily hydrolyzed to produce geraniol by acetyl esterase . Current supply of geraniol and geranyl acetate is predominantly from plant material extraction and chemical synthesis, which are either costly or unsustainable and generally unfavorable. , …”

“…6,8 Geranyl acetate, the ester derivative of geraniol, is also widely used in the cosmetic industry due to its floral and fruity scent and can also be easily hydrolyzed to produce geraniol by acetyl esterase. 9 Current supply of geraniol and geranyl acetate is predominantly from plant material extraction and chemical synthesis, which are either costly or unsustainable and generally unfavorable. 5,10 Microbial cell factories have been successfully developed to produce many value-added chemicals with great achievements.…”