To reach the video bandwidth requirements on the supply paths, power amplifiers with dynamic bias schemes are constrained to reduce the values of the low-frequency (LF) decoupling capacitors on their bias lines. This can entail a decrease of the LF stability margins, among other negative effects. In this work, a methodology is proposed to experimentally monitor and control the dominant poles that govern the LF dynamics of both gate and drain bias lines from dc to high compression power. A specific topology for the bias observation accesses allows a consistent characterization in large-signal regimes. An automatic procedure to trace root contours versus four control parameters is also developed. The complete approach can be used to optimize the design of the bias lines in terms of video bandwidth, relative stability margins, and bias voltage transfer function characteristics. Moreover, the design can account for the effect of the large-signal RF drive on these LF performances. The methodology is exemplified and validated in a demonstrator prototype specifically built for that purpose.