Combined Effect of Bilateral Ovariectomy and Anterior Cruciate Ligament Transection With Medial Meniscectomy on the Development of Osteoarthritis Model

Kim, Jae Lim; Moon, Chang-Won; Son, Young Suk

doi:10.5535/arm.2016.40.4.583

Cited by 11 publications

(8 citation statements)

References 24 publications

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“…Numerous animal models of OA have been developed to mimic pathologic human cartilage 22 . Among these different models, transection of the ACLT+MM has been one of the most widely used to induce OA, The joint instability caused by ACLT damage further leads to biological mechanics changes resemble the morphologic and biochemical changes seen in human OA 23 . ACLT+MM model provides progressive lesions that develop over time which is useful to study early stages of the OA 24 .…”

Section: Effects Of Cos On the Ratio Of Opg/rankl And Rankl/rank In Rmentioning

confidence: 99%

The effects of chitosan oligosaccharides on OPG and RANKL expression in a rat osteoarthritis model

Zhang

Jiang

et al. 2017

Acta Cir. Bras.

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Purpose: To investigate the effect of chitosan oligosaccharides (COS) against osteoarthritis (OA) and preliminarily discuss the osteoprotegerin (OPG), receptor activator of NF-κB ligand (RANKL) and RANK expression in a rat OA model. Methods: Thirty-six 6-week-old Male SD rats were randomly divided into three groups: shamoperated group(CON), OA-induction group(OA), COS intervention group(n=12/group). At 4 weeks after the operation, COS (50 ul) intervention weekily for consecutive 5 weeks. The OA and CON groups received an injection of 50 ul physiological saline. At death, 11 weeks following surgery, cartilage was harvested and total RNA and protein were extracted. Both the morphological changes of the cartilage were observed and harvested the total RNA and protein.Meanwhile, the expression of OPG, RANKL and RANK in cartilage were determined. Results: The expression of OPG and RANKL were both enhanced in the cartilage of the OA model. Compared with the OA group, COS treatment improved the cartilage damage (both extent and grade). Furthermore, the COS group showed highly OPG and lower RANKL. Simultaneously, COS treatment upregulated the ratio of OPG/RANKL and downregulated the RANKL/RANK. Conclusion: Chitosan oligosaccharides may be used as a unique biological agent to prevent and treat osteoarthritis, and this effect is associated with modulation of the expression of osteoprotegerin and receptor activator of NF-κB ligand.

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Section: Effects Of Cos On the Ratio Of Opg/rankl And Rankl/rank In Rmentioning

confidence: 99%

The effects of chitosan oligosaccharides on OPG and RANKL expression in a rat osteoarthritis model

Zhang

Jiang

et al. 2017

Acta Cir. Bras.

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“…In this work we used only male rats. Current data on ACLT in female rats is limited; in females, ACLT is mostly used in combination with ovariectomy, which was not relevant to our study . Additional similar studies should be carried out in female rats to validate the relevance of our data.…”

Section: Discussionmentioning

confidence: 84%

mTOR‐mediated inactivation of 4E‐BP1, an inhibitor of translation, precedes cartilage degeneration in rat osteoarthritic knees

Katsara

Kolupaeva

2018

Journal Orthopaedic Research

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Proper control of protein synthesis is vital for tissue homeostasis and its deregulation is characteristic of many disorders including osteoarthritis (OA). The objectives of this work were to analyze and correlate changes in activity of the translation apparatus associated with cartilage degeneration in an animal model of OA. Osteoarthritis was induced surgically in rats by anterior cruciate ligament transection (ACLT). Using a modified Mankin scoring system and analysis of protein expression we demonstrated, that mechanistic target of rapamycin complex 1 (mTORC1)-mediated 4E-BP1 phosphorylation was detected significantly earlier than other mTORC1-mediated modifications, such as p70S6K and ULK1 phosphorylation. 4E-BP1 is an inhibitor of cap-dependent translation those functions are inhibited by mTORC1 mediated phosphorylation. This signaling event not only preceded prominent signs of cartilage degeneration but also the increase in global protein synthesis rate. These results suggest that abnormal mTORC1 activity is one of the primary dysregulations observed in OA cartilage. Importantly, it is distributed disproportionately between targets, with 4E-BP1 being phosphorylated earlier than other downstream targets. Thus, our work provides new insights into the sequence of molecular events leading to cartilage destruction in OA and identifies translational control as an important regulatory hub involved in initiating OA. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:2728-2735, 2018.

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“…The study was approved by the local Ethics Committee and performed in compliance with the Guidelines for the Care and Handling of Experimental Animals of the Laboratory Animal Center, Shanghai Tongji University. The study used the murine anterior cruciate ligament transection (ACLT)-induced model of osteoarthritis (OA) [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

“…The experiments were repeated in triplicate, resulting in a total of 72 mice being used throughout the study. ACLT surgery was performed as previously described [ 16 ]. Briefly, the anterior cruciate ligament of the right knee was transected to generate a destabilized OA animal model.…”

Section: Methodsmentioning

confidence: 99%

Icariin Reduces Cartilage Degeneration in a Mouse Model of Osteoarthritis and is Associated with the Changes in Expression of Indian Hedgehog and Parathyroid Hormone-Related Protein

2018

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BackgroundThe aim of this study was to determine the role of icariin, a Chinese traditional herbal medicine extracted from Epimedium, in osteoarthritis (OA), using the murine anterior cruciate ligament transection (ACLT)-induced model of OA and micromass culture of murine chondrocytes.Material/MethodsTwenty-four three-month-old C57/6J mice were randomly divided into three groups: the sham group (no surgery and joint injection with normal saline) (N=8); the ACLT + ICA group (ACLT surgery and icariin treatment) (N=8); and the ACLT group (ACLT surgery and joint injection with normal saline) (N=8). At 12 weeks after ACLT surgery, murine articular cartilage was harvested from all mice for histological evaluation of any differences in cartilage degeneration. In vitro micromass culture of mouse chondrocytes was used to study the effects of icariin on chondrocyte differentiation and growth from the three mouse groups.ResultsIcariin treatment (mice in the ACLT + ICA group) significantly reduced degeneration of cartilage in OA with increased cartilage thickness, associated with increased expression of collagen type II alpha 1 (COL2A1), decreased chondrocyte hypertrophy, and decreased expression of collagen type X (ColX) and matrix metalloproteinase 13 (MMP13). In vitro, icariin promoted chondrocyte differentiation by upregulating the expression of agrrecan, Sox9 and parathyroid hormone-related protein (PHrP) and down-regulation of Indian hedgehog (Ihh) and genes regulated by Ihh.ConclusionsIn a mouse model of OA icariin treatment reduced destruction of cartilage, promoted chondrocyte differentiation, upregulated expression of PHrP and down-regulated the expression of Ihh.

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Combined Effect of Bilateral Ovariectomy and Anterior Cruciate Ligament Transection With Medial Meniscectomy on the Development of Osteoarthritis Model

Cited by 11 publications

References 24 publications

The effects of chitosan oligosaccharides on OPG and RANKL expression in a rat osteoarthritis model

The effects of chitosan oligosaccharides on OPG and RANKL expression in a rat osteoarthritis model

mTOR‐mediated inactivation of 4E‐BP1, an inhibitor of translation, precedes cartilage degeneration in rat osteoarthritic knees

Icariin Reduces Cartilage Degeneration in a Mouse Model of Osteoarthritis and is Associated with the Changes in Expression of Indian Hedgehog and Parathyroid Hormone-Related Protein

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