Combined effect of nitrogen and phosphorus on growth and biochemical composition of Tetradesmus obliquus (Turpin) M.J. Wynne

Abstract

Microalgae have many biotechnological applications in various industries including food and feed, fertilizer, biofuel, cosmetics, pharmaceutics, and wastewater treatment. Since hey produce secondary metabolites under stress conditions such as pigments, carotenoids, hydrocarbons, and vitamins, investigating the effects of stress factors on growth parameters and biochemical composition of microalgal biomass is needed in producing bioproducts. 
 In this paper, the combined effects of nitrogen and phosphorus on growth and the protein/amino acid and Lipid-FAMEs profiles of microalgae Tetradesmus obliquus (MAKUMACC-037) were investigated. 
 Nitrogen and phosphorus deficiency reduced the algal growth. Biochemical composition was changed in a nitrogen and phosphorus dependent manner.
 High concentration of protein and lipid were associated with increased nitrogen and phosphorus concentration However, the FAMEs profiles were changed depending on only the nitrogen concentration.

A robust multinutrient kinetic model for enhanced lutein and biomass yields in mixotrophic microalgae cultivation: A step towards successful large‐scale productions

“…The data analysis from model calibration and validation profiles (Figures 4a,b and 5a,b) demonstrates that nitrogen concentration has a significant impact on both cell growth and lutein formation, which is consistent with previous studies (Akgül & Akgül, 2022; Bentahar & Deschênes, 2022). Nitrogen plays a crucial role in algal growth, as it is a fundamental component of various biomolecules that are essential for cellular processes, including enzymes, chlorophylls, genetic material, and energy transfer molecules (Kim et al, 2016).…”

“…To validate the model, a separate set of experimental data was used, distinct from the data utilized to estimate the unknown parameters for each initial nitrate concentration, as described in Section 3.3. Specifically, the data obtained from duplicate batch experiments in The data analysis from model calibration and validation profiles (Figures 4a,b and 5a,b) demonstrates that nitrogen concentration has a significant impact on both cell growth and lutein formation, which is consistent with previous studies (Akgül & Akgül, 2022;Bentahar & Deschênes, 2022). Nitrogen plays a crucial role in algal growth, as it is a fundamental component of various biomolecules that are essential for cellular processes, including enzymes, chlorophylls, genetic material, and energy transfer molecules (Kim et al, 2016).…”

“…It is crucial to synthesize cellular components such as the cell membrane, DNA, RNA, and ATP (Atiku et al, 2016). Previous studies have shown that P deficiency in the culture medium reduces T. obliquus growth (Akgül & Akgül, 2022; Xin et al, 2010). N–P coupling has also been found to have a significant effect on nutrient uptake, dry mass, and pigments (Huang et al, 2021).…”

“…Previous studies have shown that P deficiency in the culture medium reduces T. obliquus growth (Akgül & Akgül, 2022;Xin et al, 2010).…”