Combined effects of chronic hyperglycaemia and oral aluminium intoxication on testicular tissue and some male reproductive parameters in Wistar rats

Akinola, Oluwole Busayo; Biliaminu, Sikiru Abayomi; Adedeji, Otukoya; Oluwaseun, B. S.; Olawoyin, O. M.; Adelabu, T. A.

doi:10.1111/and.12512

Cited by 11 publications

(5 citation statements)

References 29 publications

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“…This study showed a reduction of sperm concentration in MLD-STZ mice, which is similar to the results of previous investigations in MLD-STZ rats and mice ( 19 - 20 ), as well as those who underwent OHD-STZ mice model ( 16 ). This reduction may be caused by low testosterone levels and decreases in total Leydig cell numbers ( 2 , 21 ).…”

Section: Discussionsupporting

confidence: 91%

Testicular histopathology and phosphorylated protein changes in mice with diabetes induced by multiple-low doses of streptozotocin: An experimental study

Sampannang

Arun

Burawat

et al. 2018

IJRM

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Background:The streptozotocin (STZ)-induced diabetic model is widely used to evaluate the adverse effects of diabetes mellitus (DM) on spermatogenesis and testicular steroidogenesis. However, the actual mechanism of sub/infertility in DM males needs to be elucidated.Objective:To conduct a detailed examination of the testicular histopathology, sperm acrosome reaction (AR) status, and tyrosine-phosphorylated protein expression in the testis of male mice induced with STZ.Materials and Methods:Ten ICR mice were divided into two groups (n=5/each): control and diabetes induced by multiple low doses of streptozotocin (MLD-STZ). The control mice were intraperitoneally injected with citrate buffer, whereas MLD-STZ mice were injected with STZ at 40 mg/kg body weight for five consecutive days. At the end of the experiment (day 40), reproductive parameters, AR status, and the histopathology of the testis and epididymis were evaluated. The expression of testicular tyrosine phosphorylated proteins was examined.Results:Blood glucose levels, AR percentages, and sperm abnormality of STZ group were significantly higher (p=0.003, 0.001, 0.000), while sperm concentration was significantly lower (p=0.001) compared to control. Histopathology of the seminiferous tubule was classified into 7 types. Additionally, abundant round cells were found in the epididymal lumen of the MLD-STZ mice. Moreover, the intensities of testicular phosphorylated proteins (170, 70, 36, 30, and 25 kDas) were markedly higher and a 120 kDa protein band was noticeably lower in the MLD-STZ mice.Conclusion:MLD-STZ-induced DM causes many testicular histopathologies, precocious sperm AR, and increased expression of testicular phosphorylated proteins. These findings may clarify some mechanisms of sub/infertility in DM males.

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Section: Discussionsupporting

confidence: 91%

Testicular histopathology and phosphorylated protein changes in mice with diabetes induced by multiple-low doses of streptozotocin: An experimental study

Sampannang

Arun

Burawat

et al. 2018

IJRM

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“…Testicular dysfunction is a chronic disorder of unclear etiology, characterized by the destruction of the germ cells of seminiferous tubules in addition to other supplementary cells, such as Sertoli cells and Leydig cells [1]. Environmental factors, especially pesticides, confer toxic effects on testicular tissue, which explains the increased rate of reproductive toxicity during life [2].…”

Section: Introductionmentioning

confidence: 99%

Rifaximin Protects against Malathion-Induced Rat Testicular Toxicity: A Possible Clue on Modulating Gut Microbiome and Inhibition of Oxidative Stress by Mitophagy

et al. 2022

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Testicular dysfunction is caused by chronic exposure to environmental pollution, such as malathion, which causes oxidative stress, promoting cell damage. Autophagy is a key cellular process for eliminating malfunctioning organelles, such as the mitochondria (mitophagy), an eminent source of reactive oxygen species (ROS). Autophagy is crucial for protection against testicular damage. Rifaximin (RFX) is a non-absorbable antibiotic that can reshape the gut microbiome, making it effective in different gastrointestinal disorders. Interestingly, the gut microbiome produces short chain fatty acids (SCFAs) in the circulation, which act as signal molecules to regulate the autophagy. In this study, we investigated the regulatory effects of RFX on gut microbiota and its circulating metabolites SCFA and linked them with the autophagy in testicular tissues in response to malathion administration. Moreover, we divided the groups of rats that used malathion and RFX into a two-week group to investigate the mitophagy process and a four-week group to study mitochondriogenesis. The current study revealed that after two weeks of cotreatment with RFX, apoptosis was inhibited, oxidative stress was improved, and autophagy was induced. More specifically, PINK1 was overexpressed, identifying mitophagy activation. After four weeks of cotreatment with RFX, there was an increase in acetate and propionate-producing microflora, as well as the circulating levels of SCFAs. In accordance with this, the expression of PGC-1α, a downstream to SCFAs action on their receptors, was activated. PGC-1α is an upstream activator of mitophagy and mitochondriogenesis. In this sense, the protein expression of TFAM, which regulates the mitochondrial genome, was upregulated along with a significant decrease in apoptosis and oxidative stress. Conclusion: we found that RFX has a positive regulatory effect on mitophagy and mitochondria biogenesis, which could explain the novel role played by RFX in preventing the adverse effects of malathion on testicular tissue.

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“…Previous studies have reported decreased sperm motility and viability to correlate with high concentrations of AlCl 3 in spermatozoa likewise in seminal plasma. [ 28 29 ] However, in rats given AlCl 3 + QUE (Group D), 200 mg/kg of QUE treatment showed an obvious but not significant improvement in sperm count, motility, and viability. This indicates that treating rats with 200 mg/kg of QUE could mitigate AlCl 3 negative effects on sperm count, motility, and viability.…”

Section: Discussionmentioning

confidence: 99%

Observable Protective Activities of Quercetin on Aluminum Chloride-Induced Testicular Toxicity in Adult Male Wistar Rat

Afees

Akinpade

Olatunji

et al. 2021

Journal of Human Reproductive Sciences

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Background: Aluminum chloride (AlCl3 ) present in many manufactured consumable is considered as a toxic element. Aim: Our study evaluates the toxic effects induced by AlCl3 on the testes as well as the therapeutic tendency of Quercetin (QUE) agent as an antioxidant. Setting and Design: In the department of Anatomy of Medical School. Methods and Materials: Thirty-two male Wistar rats weighing approximately 170 ± 10 g were assigned into four groups with eight each, fed with rat chow and water ad-libitum. Group A served as control and was given distilled water throughout; Group B was given only QUE (200 mg/kg body weight) for 21 days; Group C was given only AlCl3 (300 mg/kg body weight) for 14 days; and Group D was given AlCl3 (300 mg/kg body weight) for 14 days followed with QUE (200 mg/kg body weight) for 21 days. Substance administrations were done orally. Statistical analysis: One-way analysis of variance was used to analyze the data, in GraphPad Prism 6.0 being the statistical software. Results: AlCl3 significantly reduced the relative organ (testes) weight, correlating the decrease in sperm count, sperm motility and sperm viability. Furthermore, there was a decrease in luteinizing hormone with an increase in follicle-stimulating hormone which accounted for a significant reduction in testosterone level that plays a great role in spermatogenesis, following AlCl3 treatment. The cytoarchitecture of the testes showed degenerative changes in the seminiferous tubules and leydin cells, nitric oxide synthases immunoreactivity was intense in the seminiferous epithelium of rat in Group C. Conclusion: These suggest that QUE antioxidant property could reverse the decrease in sperm status, hormonal effects, and functional deficit induced by aluminum chloride on the testes of Wistar rats.

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Combined effects of chronic hyperglycaemia and oral aluminium intoxication on testicular tissue and some male reproductive parameters in Wistar rats

Cited by 11 publications

References 29 publications

Testicular histopathology and phosphorylated protein changes in mice with diabetes induced by multiple-low doses of streptozotocin: An experimental study

Testicular histopathology and phosphorylated protein changes in mice with diabetes induced by multiple-low doses of streptozotocin: An experimental study

Rifaximin Protects against Malathion-Induced Rat Testicular Toxicity: A Possible Clue on Modulating Gut Microbiome and Inhibition of Oxidative Stress by Mitophagy

Observable Protective Activities of Quercetin on Aluminum Chloride-Induced Testicular Toxicity in Adult Male Wistar Rat

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