Combined effects of flow-induced shear stress and micropatterned surface morphology on neuronal differentiation of human mesenchymal stem cells

Brain cells and neuronal networks: Encounters with controlled microenvironments

“…The same trend to obtain the greatest benefits on the narrowest topographies was found for deeper (3 lm) micron sized grooves when varying their two other characteristic dimensions in the range 5-20 lm [154]. The association of both topographical and mechanical (produced by the application of a fluidic shear stress) signals has also revealed effective for the neuronal differentiation of human multipotent stem cells [154]. The morphological changes induced by either mechanical or topographical cues might be at the start of a causal chain involving successively a rearrangement of the adhesive contacts with the substrates and of the cytoskeleton structure, designating mechanosensitive pathways as key players in stem cells differentiation steps [155].…”

“…300 nm for both width and pitch) engraved in PDMS with a depth of 300 nm induced the highest induction and differentiation effects [153]. The same trend to obtain the greatest benefits on the narrowest topographies was found for deeper (3 lm) micron sized grooves when varying their two other characteristic dimensions in the range 5-20 lm [154]. The association of both topographical and mechanical (produced by the application of a fluidic shear stress) signals has also revealed effective for the neuronal differentiation of human multipotent stem cells [154].…”

Heart-on-a-chip based on stem cell biology

“…Moreover, stem cells can differentiate under influence of shear stress into osteogenic (Kreke et al, 2008) and neural cells (Jeon et al, 2014).…”

“…The effect of shear stress on stem cell differentiation in microfluidic systems was investigated on various kind of stem cells (Jeon et al, 2014, Villa-Diaz et al, 2009, Xiao et al, 2014, Figallo et al, 2007. hESC colonies were cultured in dynamic (flow applied) or static (without flow) conditions in the microsystem, which consists of three inlet channels that converge into a cell-culture channel.…”

An academic, clinical and industrial update on electrospun, additive manufactured and imprinted medical devices

“…Imprinted arrays of topographical patterns have been utilised extensively in vitro and are recognised as potent tools in maintaining cell phenotype [91,100] and in differentiating stem cells towards osteogenic [101], neurogenic [102], adipogenic [103] and myogenic [104] lineages. The cellular mechanisms that regulate topography-mediated differential cell function may be direct [105] (the physical influence of topography) and/or indirect (the chemical influence of topography, as a result of changes to the composition, orientation or conformation of adsorbed extracellular matrix components) [106,107].…”