Combined effects of salinity, temperature, hypoxia, and Deepwater Horizon oil on Fundulus grandis larvae

Serafin, Jennifer; Guffey, Samuel C.; Bosker, Thijs; Griffitt, Robert J.; Guise, Sylvain De; Perkins, Christopher; Szuter, Michael; Sepúlveda, Marı́a S.

doi:10.1016/j.ecoenv.2019.05.059

Cited by 22 publications

(12 citation statements)

References 42 publications

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“…Previous research has demonstrated that co-exposure to oil and hypoxia impacts developing C. variegatus as well as Fundulus grandis [14][15][16][17] and can reduce egg production and fertilization in adults 18 . Despite the fact that both oil and hypoxia are known to affect developing C. variegatus, and that both stressors are known to affect fish immune systems, little is known about how these two stressors interact to impact immune system development.…”

mentioning

confidence: 99%

Exposure to Oil and Hypoxia Results in Alterations of Immune Transcriptional Patterns in Developing Sheepshead Minnows (Cyprinodon variegatus)

Rodgers

Simning

Sepúlveda

et al. 2020

Sci Rep

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the area and timing of the Deepwater Horizon oil spill highlight the need to study oil and hypoxia exposure in early life stage fishes. Though critical to health, little research has targeted the effect of oil and hypoxia exposure on developing immune systems. to this end, we exposed sheepshead minnows (Cyprinodon variegatus) at three early life stages: embryonic; post-hatch; and post-larval, to a high energy water accommodated fraction (HEWAF) of oil, hypoxia, or both for 48 hours. We performed RnAseq to understand how exposures alter expression of immune transcripts and pathways. Under control conditions, the embryonic to post-hatch comparison (first transition) had a greater number of significantly regulated immune pathways than the second transition (post-hatch to post-larval). The addition of oil had little effect in the first transition, however, hypoxia elicited changes in cellular and humoral immune responses. In the second transition, oil exposure significantly altered many immune pathways (43), and while hypoxia altered few pathways, it did induce a unique signature of generally suppressing immune pathways. these data suggest that timing of exposure to oil and/or hypoxia matters, and underscores the need to further investigate the impacts of multiple stressors on immune system development in early life stage fishes. The functionality of the immune system is key to organismal health and survival. Alteration of the immune system can have serious consequences, including immunosuppression or autoimmunity. The immune system is so critical to life that it starts developing very early on: for example, the innate immune system in zebrafish (Danio rerio) is detectable at day 1 of embryogenesis (via the appearance of macrophages) 1,2 and embryonic zebrafish exposed to Salmonella typhimurium respond with induction of two matrix metalloproteinase genes (mmp9 and mmp13) 3. In addition, the zebrafish immune system is both morphologically and functionally mature 4-6 weeks after fertilization 4. However, since fish are a highly diverse taxa, this period of development can widely vary depending upon species and environmental conditions. The area of oiling from the 2010 Deepwater Horizon oil spill impacted sites where embryos and other developing fishes reside 5. Given the fact that the immune system develops during early life stages, it is important to examine the immunological consequences of oil exposure on developing fish. In addition to contending with oil exposure, the Deepwater Horizon oil spill took place in areas that are prone to experiencing hypoxic conditions 6,7. Literature has clearly established that exposure to polycyclic aromatic hydrocarbons (PAHs) (for example, via oil spills) results in reduced immune function in multiple fish species 8 , but there is also evidence of immunological effects of hypoxia exposure in fishes. For example, fish experiencing hypoxic conditions can have decreased complement activity 9 , reduced production of reactive oxygen species 10 , depressed respiratory burst activity

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confidence: 99%

Exposure to Oil and Hypoxia Results in Alterations of Immune Transcriptional Patterns in Developing Sheepshead Minnows (Cyprinodon variegatus)

Rodgers

Simning

Sepúlveda

et al. 2020

Sci Rep

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“…Magnuson et al (2018) also reported impaired ocular development in Red Drum and Sheepshead Minnow (Cyprinodon variegatus) larvae, which resulted in observable reductions in behavioral (optomotor) responses to external stimuli. In addition, numerous studies have reported significant impacts at the genomic level after oil exposure, resulting in alterations of gene expression, organ development, cellular pathways, and immune responses (e.g., Whitehead et al, 2012;Dubansky et al, 2013), as well as the combined effects of oil exposure with variable environmental factors, such as dissolved oxygen, temperature, salinity and light exposure (e.g., Alloy et al, 2017;Serafin et al, 2019;Rodgers et al, 2020). Collectively, these sublethal effects suggest that fish larvae exposed to DWH oil in situ would have had impaired survival abilities needed to evade predators and locate prey.…”

Section: Species-level Summariesmentioning

confidence: 99%

A Synthesis of Deepwater Horizon Impacts on Coastal and Nearshore Living Marine Resources

et al. 2021

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The 2010 Deepwater Horizon (DWH) oil blowout in the Gulf of Mexico began on April 20, originating in the deep sea 66 km off the Louisiana coast. By early June, DWH oil had spread to coastal Louisiana, Mississippi, Alabama and western Florida. An estimated 2,113 km of shoreline were oiled, making DWH the largest marine oil spill in global history by length of affected shoreline. Additionally, a series of oil spill response measures were deployed, including diversions of Mississippi River discharge to forestall oil coming ashore, and the establishment of large-scale fishery closures, with both affecting coastal resources to varying degrees. Here, we review published studies and describe additional analyses evaluating long-term impacts of DWH on coastal/nearshore biological resources. We assembled time-series data collected by state, federal and academic partners on population abundance and environmental conditions to evaluate species and community change. Our study focused on plankton, invertebrates, fishes and dolphins, and 13 “key species” were selected to conduct semi-quantitative vulnerability-resilience (V-R) analyses. At one extreme, early life stages of Gulf Menhaden (Brevoortia patronus) were not affected due to seasonal spawning and larval development preceding the spill. In contrast, demographically independent populations of the common Bottlenose Dolphin, (Tursiops truncatus) suffered a variety of severe and ongoing health effects owing to oil exposure. Virtually all of the heavily oiled salt marsh habitat was in Louisiana, with the majority occurring in Barataria Bay. Multispecies trawl survey abundances declined post-DWH throughout eastern coastal Louisiana but remained stable elsewhere. A regime shift in composition of Barataria Bay trawl survey catches occurred during and following the spill, the persistence of which was associated with long-term reductions in average salinity and increases in water clarity. In some cases, fishery closures were associated with measurable but ephemeral increases in abundance of some targeted and bycatch species. Freshwater flooding of marshes was ineffective in preventing coastal oiling and severely affected benthic euryhaline resources including Eastern Oyster (Crassostrea virginica) and Marsh Periwinkle (Littoraria irrorata). The flooding response measure experiment also indicates the directionality of impacts that further planned water diversions may have on ecological communities of lower Mississippi River basins.

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“…PAH concentrations from HEWAF dilutions and test solutions over time were calculated using fluorescence methodology by measuring fluorescence between 270 and 380 nm (Greer et al, 2012). See Serafin et al (2019), Table S2 for total PAH concentrations and the 29 analytes quantified (tPAH29).…”

Section: Pah Analysismentioning

confidence: 99%

“…PAH concentrations were indirectly quantified through fluorometry (Greer et al, 2012) using a Turner Designs AU-10 fluorometer (Turner Designs, San Jose, CA, USA). For full water chemistry methodology see Serafin et al (2019).…”

Section: Water Chemistrymentioning

confidence: 99%

“…While many studies have identified the toxicological effects of oil in estuarine and marine fishes (for a review, see Murphy et al, 2016), only recently has work begun to identify the combined effects of oil and abiotic environmental factors such as hypoxia, salinity, and temperature (Hedgpeth and Griffitt, 2016;Jasperse et al, 2019;Mauduit et al, 2018;Milinkovitch et al, 2020;Serafin et al, 2019;Simning et al, 2019). Specifically, a number of studies have found impacts of abiotic stressors on the developing cardiac system in fish.…”

Section: Introductionmentioning

confidence: 99%

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Effects of polycyclic aromatic hydrocarbons and abiotic stressors on Fundulus grandis cardiac transcriptomics

Allmon

Serafin

Chen

et al. 2021

Science of The Total Environment

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Combined effects of salinity, temperature, hypoxia, and Deepwater Horizon oil on Fundulus grandis larvae

Cited by 22 publications

References 42 publications

Exposure to Oil and Hypoxia Results in Alterations of Immune Transcriptional Patterns in Developing Sheepshead Minnows (Cyprinodon variegatus)

Exposure to Oil and Hypoxia Results in Alterations of Immune Transcriptional Patterns in Developing Sheepshead Minnows (Cyprinodon variegatus)

A Synthesis of Deepwater Horizon Impacts on Coastal and Nearshore Living Marine Resources

Effects of polycyclic aromatic hydrocarbons and abiotic stressors on Fundulus grandis cardiac transcriptomics

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