Combined effects of Salmonella phage cocktail and organic acid for controlling Salmonella Enteritidis in chicken meat

Pelyuntha, Wattana; Vongkamjan, Kitiya

doi:10.1016/j.foodcont.2021.108653

Cited by 20 publications

(22 citation statements)

References 57 publications

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“…Most phages also show diverse bacteriolytic patterns against many pathogenic bacteria [39][40][41][42][43][44]. Salmonella phages have also been recovered from wastewater and sewage [15,19,45]. The diversity of phage lytic activity depends on the source of phage origin.…”

Section: Discussionmentioning

confidence: 99%

“…The diversity of phage lytic activity depends on the source of phage origin. Some Salmonella phages exhibited a narrow host range or serovar-specific [46,47], whereas others exhibited a diverse pattern of lytic ability [15,19]. However, phages with a broad lytic activity are preferred to be used as a biocontrol to kill Salmonella.…”

Section: Discussionmentioning

confidence: 99%

“…The enrichment isolation method was used for Salmonella phage isolation utilizing the mixture of three Salmonella strains in our laboratory culture collection (Salmonella Agona H2-016, Salmonella Anatum A4-525, and Salmonella Kentucky S1H28). Enrichment and isolation steps were conducted following a principal protocol from our laboratory [15]. Plaques were evaluated on the bacterial host lawn, and the plaque morphotype was measured using a Vernier caliper.…”

Section: Isolation Purification and Preparation Of Salmonella Phagesmentioning

confidence: 99%

“…The supernatant was obtained by centrifugation at 6000 rpm for 15 min at 4°C, followed by filtration through 0.20 µm syringe filters before storing at 4°C. The titer of phages was counted using the double overlay technique [15].…”

Section: Isolation Purification and Preparation Of Salmonella Phagesmentioning

confidence: 99%

“…Bacteriophages or phages are viruses that can specifically kill bacteria and are not harmful to human and animal health. Phages are widely used to treat various pathogenic and MDR bacteria with strong bactericidal effects and high specificity [15]. In addition, phages can be used as an alternative approach for combatting CIP-resistant Salmonella [16].…”

Section: Introductionmentioning

confidence: 99%

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Broad lytic spectrum of novel Salmonella phages on ciprofloxacin-resistant Salmonella contaminated in the broiler production chain

et al. 2022

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Background and Aim: Ciprofloxacin (CIP) is recommended for salmonellosis treatment as the drug of choice; however, overuse of this drug can cause drug resistance issues and failure to treat diseases. Phage therapy is an alternative approach for combatting CIP-resistant infection. This study aimed to estimate the prevalence of CIP-resistant Salmonella isolated from the broiler production chain and evaluated the lytic ability of novel Salmonella phages isolated from water samples. Materials and Methods: Samples were obtained from the broiler production chain and used for Salmonella isolation. serovar and CIP resistance of each isolate were characterized through latex agglutination and agar disk diffusion test, respectively. Water samples from different sources were acquired for phage isolation. The lytic activity of novel-isolated phages was also examined. Results: In this study, 51 Salmonella isolates were recovered from the broiler production chain (two commercial farms, one free-range farm, two slaughterhouses, and three stalls from the wet market). Kentucky was the major serovar characterized (16), followed by Typhimurium (9), Agona (5), Corvalis (5), Schwarzengrund (5), Singapore (3), Weltevreden (3), Mbandaka (2), Give (2), and Albany (1). The serovars that exhibited CIP resistance were 14/16 isolates of serovar Kentucky (87.5%) and one isolate of serovar Give (50%), whereas eight other serovars were susceptible to this drug. Overall, the prevalence of CIP-resistant Salmonella recovered from the sources included in this study was 29.4%. This study identified 11 Salmonella phages isolated from wastewater samples derived from broiler farms, wastewater treatment stations, and natural reservoirs. Our phages showed the total percentage of lysis ability ranging from 33.3% to 93.3% against CIP-resistant isolates. However, only one bacterial isolate, namely 210SL, recovered from the food contact surface of a wet market stall and was resistant to all phages. Conclusion: Diverse serovars of Salmonella were recovered in the broiler production chain in this study, while the isolates presenting CIP-resistant Salmonella were as high as 29.4%. Overall, Salmonella phages showed high lysis ability against these CIP-resistant Salmonella isolates, suggesting the potential application of phage-based treatments or biocontrol in the broiler production chain.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Isolation Purification and Preparation Of Salmonella Phagesmentioning

confidence: 99%

Section: Isolation Purification and Preparation Of Salmonella Phagesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Broad lytic spectrum of novel Salmonella phages on ciprofloxacin-resistant Salmonella contaminated in the broiler production chain

et al. 2022

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Characterization of Salmonella phages isolated from poultry coops and its effect with nisin on food bio‐control

Unverdi,

Erol,

Kaskatepe

et al. 2024

Food Science & Nutrition

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Salmonella is a bacterium associated with food contaminated by various animals, primarily poultry. Interest and research on bacteriophages are increasing because they can be used as an alternative against increasing antibiotic resistance. In our study, eight Salmonella‐specific lytic bacteriophages were isolated from chicken feces. Two of the isolated phages (AUFM_Sc1 and AUFM_Sc3) were chosen for their characterization due to their broader host range. Based on morphological and genomic analysis, AUFM_Sc1 was identified to be close to similar Enterobacteria spp. CC31 (Myoviridae) and AUFM_Sc3 was identified to be close to Salmonella phage vB_Sen_I1 (Demerecviridae (formerly Siphoviridae)). Although these phages have shown promise for use in phage therapy applications for chickens, further studies are needed on their suitability. When a cocktail of these phages (AUFM_Sc1 + AUFM_Sc3) and nisin combination was applied on chicken breast meat, it was determined that it was effective against Salmonella contamination and while a good inhibitory effect was observed on the food, especially during the first 48 h, the effect decreased later, but the bacterial concentration was still low compared to the control group. Therefore, it is considered that the combination of AUFM_Sc1 + AUFM_Sc3 + nisin can be used as a food preservative against Salmonella.

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Inhibition of Salmonella Enteritidis adhesion and biofilm formation by β-glucosidase B from Microbacterium sp. BG28

Atanaskovic,

Moric,

Rokic

et al. 2024

Food Bioscience

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Combined effects of Salmonella phage cocktail and organic acid for controlling Salmonella Enteritidis in chicken meat

Cited by 20 publications

References 57 publications

Broad lytic spectrum of novel Salmonella phages on ciprofloxacin-resistant Salmonella contaminated in the broiler production chain

Broad lytic spectrum of novel Salmonella phages on ciprofloxacin-resistant Salmonella contaminated in the broiler production chain

Characterization of Salmonella phages isolated from poultry coops and its effect with nisin on food bio‐control

Inhibition of Salmonella Enteritidis adhesion and biofilm formation by β-glucosidase B from Microbacterium sp. BG28

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