Combined extracts of Moringa oleifera, Murraya koeingii leaves, and Curcuma longa rhizome increases energy expenditure and controls obesity in high-fat diet-fed rats

Abstract

Background: LI85008F is a proprietary combination of leaf extracts of Moringa oleifera, Murraya koeingii, and extract of Curcuma longa rhizome. This herbal extract combination is an effective weight loss supplement for overweight and obese subjects. The present study aimed to investigate the thermogenic potential of the LI85008F in high-fat diet (HFD)-induced obese Sprague Dawley rats. Methods: Seven rats received a regular diet (RD), and twenty-one rats received a high-fat diet (HFD) for 56 days. On day 28, the HFD-fed rats were randomized into three groups (n = 7). Starting from day 29 through day 56, one HFD-fed group received daily oral gavage of 0.5% Carboxymethylcellulose Sodium (CMC) alone (HFD), and the remaining two groups received 100 and 250 mg/kg LI85008F (LI85008F-100 and LI85008F-250, respectively). Body weight, fat mass, fat cell size, liver weight, liver triglyceride were measured. The energy metabolism parameters were measured using indirect calorimetry. In serum, the metabolic and endocrine markers were analyzed. The adipogenic and thermoregulatory proteins expression in the white adipose tissue (WAT) were analyzed using an immunoblot assay. Results: Supplementation with both doses of LI85008F significantly increased resting energy expenditure (REE) in the obese rats. The LI85008F-250 rats showed significant up-regulation of uncoupling protein-1 (UCP-1) expression, as compared with the HFD rats. LI85008F significantly reduced body weight gain, fat mass, fat cell size, liver weight, and hepatic triglycerides. Serum triglyceride, total cholesterol, glucose, leptin, and fat cell markers were significantly reduced in LI85008F-supplemented rats compared to the HFD rats. Conclusion: The present data suggest that LI85008F reduces body fat mass and controls body weight gain via increasing energy metabolism in combination with reduced lipogenesis in diet-fed obese rats.

A synergistic blend of Garcinia mangostana fruit rind and Cinnamomum tamala leaf extracts enhances myogenic differentiation and mitochondrial biogenesis in vitro and muscle growth and strength in mice

“…The western blot assays were performed following the methodology described earlier with some modifications ( 23 ). Briefly, an equal amount of cell or tissue lysate proteins was resolved using 7.5 or 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).…”

The Potency of Moringa oleifera Lam. as Protective Agent in Cardiac Damage and Vascular Dysfunction

“…A study using an herbal extract combination comprised of 60% MO leaves ethanolic extract in doses of 100 and 250 mg/kg BW for 28 days significantly reduced BW gain, total body fat mass, the fat cell size of inguinal and epididymal white adipose tissue (WAT), liver weight, and hepatic triglycerides and increased resting energy expenditure (REE) and fat oxidation on obese rats. The decrease of fat cell size and mass in supplemented obese rats was due to the increase energy metabolism through browning of WAT, the increase of expression of UCP1, and down-regulation expression of key adipogenic marker proteins (PPARγ, C/EBPα, CD36, aP-2α, and perilipin ( Kundimi et al, 2020 ). However, they found that this herbal combination did not substantially affect the interscapular BAT fat mass of obese rats.…”

Moringa oleifera: Recent Insights for Its Biochemical and Medicinal Applications

“…On the other hand, MO leaves have been recorded to elevate the plasma insulin such as growth factor I (IGF-I) [271] insulin [144] and leptin hormones [42,184] leading to inhibiting adipogenesis [180,272]. On the contrary, others recorded its ability to reduce serum leptin [152,181,273,274] leading to an increase in the energy expenditure and controlling obesity [275].…”