Combined FT-Raman and SEM Studies of the Effects of Er:YAG Laser Irradiation on Dentin

Soares, Luís Eduardo Silva; Resende, Erick B.P.S.; Brugnera, Aldo; Zanin, Fátima A. A.; Martin, Aírton Abrahão

doi:10.1089/pho.2007.2054

Cited by 30 publications

(45 citation statements)

References 30 publications

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“…Er:YAG laser irradiation is generally considered to produce less thermal damage of organic tissue is lower than irradiation by other lasers 8,9) . However, the results of the present study indicate that Er:YAG laser irradiation causes chemical changes to the dentin surface [25][26][27] , and that the dentin surface is denatured. The denatured dentin surface typically exhibited a scaly surface and the collagen fibrils were melted.…”

Section: Discussioncontrasting

confidence: 63%

XPS analysis of the dentin irradiated by Er:YAG laser

et al. 2009

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The aim of this study was to investigate the effect of Er:YAG laser irradiation on human dentin surface using X-ray photoelectron spectroscopy (XPS). 10 human dentin disks were prepared from extracted human molars for XPS analysis. These specimens were divided into two groups of five: a control group and group that were irradiated by an Er:YAG laser beam (100 mJ, 1Hz). All specimens were analyzed by XPS over a wide scanning range and narrow scanning ranges. The Ca/P ratio was calculated from the XPS results.In the results, the binding energies of Ca, P, and N in the laser-irradiated group were higher than those in the control group. The Ca/P ratio of the Er:YAG laser irradiated group (1.24±0.05) was significantly lower than that of the control group (1.52±0.16). This study showed that Er:YAG laser irradiation decreased Ca/P ratio and denatured the collagen of human dentin.

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Section: Discussioncontrasting

confidence: 63%

XPS analysis of the dentin irradiated by Er:YAG laser

et al. 2009

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“…3C and 4C). An affected layer was described by Soares et al, 10 who noticed it after using the same energy density used in this study. Therefore, organic components, such as collagen fibers, could also be eliminated by NaOCl treatment.…”

Section: Discussionsupporting

confidence: 61%

“…At present, the effect of Er:YAG laser on organic components, such as collagen fibers, remains unclear. Nevertheless, Soares et al 10 have shown, using spectroscopy, that the laser treatment affected the organic substance. Furthermore, Ceballos et al 24 have shown a 3-5 lm thick laser-modified layer, an alteration of the dentin subsurface, beneath which collagen fibrils appear to have lost crossbanding and are fused together, thereby eliminating interfibrillar spaces.…”

Section: Discussionmentioning

confidence: 99%

“…To ensure good adhesion, the dentinal surface is conditioned by acid etching to dissolve this layer, which could occlude dentinal tubules and which is sometimes incorporated into the bonding of the restorations. 10 The acid effect creates a clean dentinal surface without any smear layer, and exposes collagen for adequate bonding. 11 The principle of dentine adhesion is based on micromechanical retention.…”

Section: Introductionmentioning

confidence: 99%

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Influence of Sodium Hypochlorite on Er:YAG Laser-Irradiated Dentin and its Effect on the Quality of Adaptation of the Composite Restoration Margins

Lahmouzi

Farache

Umana

et al. 2012

Photomedicine and Laser Surgery

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Objective: The aims of this in vitro study were to evaluate: (1) the influence of 5% NaOCl application on Er:YAGirradiated dentin; and (2) its effect on the quality of adaptation of the composite restoration margins. Background data: Previous research has shown that Er:YAG dentin irradiation produces a thermally affected tissue layer that results in lower bond strength than that of nonirradiated dentin. The removal of this thermally-affected layer may enhance the quality of dentin bonding Materials and methods: Forty-nine caries-free extracted human molars were transversely sectioned in order to totally expose the dentin. Four standardized cavities were created on the dentinal surface of each molar. First, two cavities were irradiated with Er:YAG laser (2.94 nm): 150 mJ, 10 Hz, variable square pulse (VSP) mode (100 lsec), beam diameter = 0.9 mm, speed of irradiation = 1 mm/sec, 20% air and 20% water. Then, one of irradiated cavities and one of nonirradiated cavities were treated for 30 sec with 5% NaOCl solution. Finally, they went through a standard bonding treatment for composite restoration, etching, bonding, and composite filling. We obtained four groups of cavities: (1) one control group of nonirradiated cavities not pretreated with NaOCl; (2) one group of nonirradiated cavities, pretreated with NaOCl; (3) one group of irradiated cavities, not pretreated with NaOCl; and (4) one group of irradiated cavities, pretreated with NaOCl. All samples were subjected to thermocycling. Every cavity was immersed into a 0.5% solution of methylene blue. The percentage of dye penetration (microleakage) in the composite-dentin interface was evaluated. Six molars were analyzed by scanning electron microscope. Results: Dye infiltration depth was significantly reduced in irradiated cavities treated with 5% NaOCl solution. Conclusions: The application of a 5% NaOCl solution on Er:YAG irradiated cavities can significantly improve the marginal quality of composite bonding.

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“…It was sliced in a disc form with 4 mm of thickness and had been already characterized in a previous study [22]. The in vivo setup was tested on human skin of two voluntary students.…”

Section: Methodsmentioning

confidence: 99%

Shifted-excitation Raman difference spectroscopy for in vitro and in vivo biological samples analysis

Martins

Ribeiro

Santos

et al. 2010

Biomed. Opt. Express

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The contamination of the Raman scattering signal with luminescence is a well-known problem when dealing with biological media excited by visible light. The viability of the shifted-excitation Raman difference spectroscopy (SERDS) technique for luminescence suppression on Raman spectra of biological samples was studied in this work. A tunable Lithrow-configuration diode laser (λ = 785 and 830 nm) coupled (directly or by optical fiber) to a dispersive Raman spectrometer was employed to study two sets of human tissues (tooth and skin) in order to determine the set of experimental parameters suitable for luminescence rejection. It was concluded that systematic and reproducible spectra of biological interest can be acquired by SERDS.

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Combined FT-Raman and SEM Studies of the Effects of Er:YAG Laser Irradiation on Dentin

Abstract: Er:YAG laser irradiation with 180 mJ could produce chemical changes in proteins, phosphate, and carbonate in dentin.

Cited by 30 publications

References 30 publications

XPS analysis of the dentin irradiated by Er:YAG laser

XPS analysis of the dentin irradiated by Er:YAG laser

Influence of Sodium Hypochlorite on Er:YAG Laser-Irradiated Dentin and its Effect on the Quality of Adaptation of the Composite Restoration Margins

Shifted-excitation Raman difference spectroscopy for in vitro and in vivo biological samples analysis

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