Combined high-performance liquid chromatography and radioimmunoassay for ceruletide and its metabolites in dog plasma and urine

Kominami, Goro; Okabe, Hiroshi; Imoda, Keiko; Mizojiri, Kenji

doi:10.1016/0731-7085(94)90019-1

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…Due to the short half-life of caerulein [35], 6 male Sprague Dawley rats per group (obtained from Charles Rivers laboratories 11–12 weeks at study start) were treated with 3 intraperitoneal doses of vehicle or caerulein (Sigma Aldrich), 1 h apart, at 0, 15 and 50 μg/kg. Blood was collected at 1, 4, 8, 24 and 48 h after dosing and examined for clinical chemistry, hematology and microRNA changes with terminal serum taken at 8, 24 and 48 h. Urine was collected by cystocentesis at 0, 4, 8, 24 and 48 h and examined for total protein and specific gravity.…”

Section: Methodsmentioning

confidence: 99%

The Rat microRNA body atlas; Evaluation of the microRNA content of rat organs through deep sequencing and characterization of pancreas enriched miRNAs as biomarkers of pancreatic toxicity in the rat and dog

et al. 2016

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BackgroundMicroRNAs (miRNA) are ~19–25 nucleotide long RNA molecules that fine tune gene expression through the inhibition of translation or degradation of the mRNA through incorporation into the RNA induced silencing complex (RISC). MicroRNAs are stable in the serum and plasma, are detectable in a wide variety of body fluids, are conserved across veterinary species and humans and are expressed in a tissue specific manner. They can be detected at low concentrations in circulation in animals and humans, generating interest in the utilization of miRNAs as serum and/or plasma based biomarkers of tissue injury. MicroRNA tissue profiling in rodents has been published, but sample an insufficient number of organs of toxicologic interest using microarray or qPCR technologies for miRNA detection. Here we impart an improved rat microRNA body atlas consisting of 21 and 23 tissues of toxicologic interest from male and female Sprague Dawley rats respectively, using Illumina miRNA sequencing. Several of the authors created a dog miRNA body atlas and we collaborated to test miRNAs conserved in rat and dog pancreas in caerulein toxicity studies utilizing both species.ResultsA rich data set is presented that more robustly defines the tissue specificity and enrichment profiles of previously published and undiscovered rat miRNAs. We generated 1,927 sequences that mapped to mature miRNAs in rat, mouse and human from miRBase and discovered an additional 1,162 rat miRNAs as compared to the current number of rat miRNAs in miRBase version 21. Tissue specific and enriched miRNAs were identified and a subset of these miRNAs were validated by qPCR for tissue specificity or enrichment. As an example of the power of this approach, we have conducted rat and dog pancreas toxicity studies and examined the levels of some tissue specific and enriched miRNAs conserved between rat and dog in the serum of each species. The studies demonstrate that conserved tissue specific/enriched miRs-216a-5p, 375-3p, 148a-3p, 216b-5p and 141-3p are candidate biomarkers of pancreatic injury in the rat and dog.ConclusionsA microRNA body atlas for rat and dog was useful in identifying new candidate miRNA biomarkers of organ toxicity in 2 toxicologically relevant species.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-2956-z) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

The Rat microRNA body atlas; Evaluation of the microRNA content of rat organs through deep sequencing and characterization of pancreas enriched miRNAs as biomarkers of pancreatic toxicity in the rat and dog

et al. 2016

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Reversed-Phase Chromatography

Schlüter

2000

Journal of Chromatography Library

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Dose-related effects of cerulein short infusions on proximal small bowel motility in sheep

Romanski¹

2010

J. S. Afr. Vet. Assoc.

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The effect of cholecystokinin (CCK) upon the intestinal motility has not been entirely explored in ruminants. The aim of this study was to examine the precise effects of CCK amphibian analogue, cerulein, on small-intestinal myoelectric activity in rams in the course of chronic experiments. Five rams underwent implantation of bipolar platinum electrodes to the duodenal bulb, the distal duodenum and jejunum. During continuous myoelectrical and motor recordings, 0.15 M NaCl or the various doses of cerulein were administered intravenously. Short infusions of the smallest dose of cerulein exerted a slight and mostly insignificant effect on the duodenal bulb and the duodenal myoelectric activity index (MAI) values. In the duodenal bulb, the effects of cerulein on myoelectric activity were dose-dependent and closely related to the phase of the MMC. In the duodenum, the higher doses of the hormone evoked short stimulatory response followed by longer inhibitory biphasic effects on MAI. These effects were inversely related to the duration of hormone injection. Infusions of hormones at the higher doses caused a less pronounced biphasic effect. It is concluded that cerulein exerts an inhibitory effect upon the myoelectric activity of the duodenal bulb and a strong stimulatory and inhibitory (biphasic) effect on duodenal motility in sheep.

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Combined high-performance liquid chromatography and radioimmunoassay for ceruletide and its metabolites in dog plasma and urine

Cited by 3 publications

References 6 publications

The Rat microRNA body atlas; Evaluation of the microRNA content of rat organs through deep sequencing and characterization of pancreas enriched miRNAs as biomarkers of pancreatic toxicity in the rat and dog

The Rat microRNA body atlas; Evaluation of the microRNA content of rat organs through deep sequencing and characterization of pancreas enriched miRNAs as biomarkers of pancreatic toxicity in the rat and dog

Reversed-Phase Chromatography

Dose-related effects of cerulein short infusions on proximal small bowel motility in sheep

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