Combined in vitro and in vivo investigation of barite microcrystals in Spirogyra (Zygnematophyceae, Charophyta)

Barbosa, Natercia; Jaquet, Jean-Michel; Urquidi, Oscar; Adachi, Takuji; Filella, Montserrat

doi:10.1016/j.jplph.2022.153769

Cited by 4 publications

(5 citation statements)

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“…The paper provides the first evidence of intracellular formation of ordered dolomite in the filamentous green alga Spirogyra varians (Zygnematales, Chlorophyta). Spirogyra is one of the dominant genera in continental water bodies and is used as a model organism to study the algal system worldwide (Lee, 2008); although few studies have focused on intracellular biomineralization in this alga (Barbosa et al ., 2022). Biomineralization processes are even less well‐known in the colonies that proliferate in highly alkaline and Mg‐rich lakes.…”

Section: Discussionmentioning

confidence: 99%

“…It is known that Ba 2+ cations are absorbed to negatively charged sites on the algae walls (Krejci et al ., 2011). The ability of different genera of green algae ( Chlorophyta ), including Spirogyra and Desmids , to accumulate baryte within their cells have been reported by a number of authors (Kreger & Boere, 1969; Krejci et al ., 2011; Barbosa et al ., 2022). However, at this stage, the exact location of baryte crystals associated in vivo with the algal filaments has not been identified in Caballo Alba deposits.…”

Section: Discussionmentioning

confidence: 99%

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Biomineralization of ordered dolomite and magnesian calcite by the green alga Spirogyra

Buey

Sanz‐Montero

2022

Sedimentology

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The formation of ordered dolomite under Earth's surface conditions remains a challenge, although many researchers have now shown that microbes can facilitate the precipitation of this common mineral. This study provides the first evidence of dolomite biomineralization by the green alga Spirogyra (Zygnematales, Chlorophyta), a globally dispersed lacustrine genus. Microbial mats formed by Spirogyra occur in the hyperalkaline and ephemeral lake Caballo Alba, located in Central Spain and characterized by water solutions with high Mg/Ca ratios. Microscopic, geochemical, mineralogical and cytochemical studies have shown that accumulations of carbonates occur within different cells and locations of the alga. Biominerals mostly consist of single crystals of magnesian calcite and dolomite with variable proportions of Ca 2+ and Mg 2+ . High-resolution transmission electron microscopy and selected area electron diffraction patterns of the carbonates confirm the pervasive presence of super-lattice reflections typical of ordered dolomite in compositions with greater than 32 mol% MgCO 3 . The curved surfaces of some carbonate crystals and their attachment to the organic surfaces represent imprints of a confined growth in compartments that act as a template for crystal nucleation and shape the crystals. Dyes reveal chemical differences in these compartments which explain the polymineral biomineralization and suggest that the alga do not strictly control the precipitation process. Zygnematales algae related with Spirogyra date back possibly to the Early Palaeozoic, thus suggesting that dolomite biomineralization could have been a common process through the Phanerozoic. This discovery provides new insight into the biotic formation of orderly dolomite and aids in the reconstructions of past lacustrine environments of dolomite deposition.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Biomineralization of ordered dolomite and magnesian calcite by the green alga Spirogyra

Buey

Sanz‐Montero

2022

Sedimentology

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“…Barite crystals have been described in vegetative samples of Spirogyra sp. (Kreger and Boere, 1969;Barbosa et al, 2022), thus we hypothesise that barite crystals are also present in Spirogyra sp. zygospores.…”

Section: Introductionmentioning

confidence: 87%

“…While biomineralization of dolomite by Spirogyra sp. has been described recently (Del Buey and Sanz-Montero, 2023), several studies report on the occurrence of barite crystals in vegetative Spirogyra sp (Kreger and Boere, 1969;Barbosa et al, 2022). Ba 2+ ions are usually toxic as they have the capacity to block K + channels (Pilatováet al, 2023), but BaSO 4 (barite) is insoluble and thus not toxic any more.…”

Section: Barite Crystals In Mature Zygosporesmentioning

confidence: 99%

“…'Tyrol' (Figure 5C) in 'secondary vacuoles' and compartments that resulted from degradation of chloroplasts. This is in contrast to the suggestion that barite crystals are formed in the cytoplasm of vegetative Spirogyra sp., as they are transported with the cytoplasmic streaming (Barbosa et al, 2022). Other than a contribution to barium detoxification (Niedermeier et al, 2018), the function of barite crystals in Zygnematophyceae remains largely unknown.…”

Section: Barite Crystals In Mature Zygosporesmentioning

confidence: 99%

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Zygospore development of Spirogyra (Charophyta) investigated by serial block-face scanning electron microscopy and 3D reconstructions

Antreich,

Permann,

Xiao

et al. 2024

Front. Plant Sci.

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Sexual reproduction of Zygnematophyceae by conjugation is a less investigated topic due to the difficulties of the induction of this process and zygospore ripening under laboratory conditions. For this study, we collected field sampled zygospores of Spirogyra mirabilis and three additional Spirogyra strains in Austria and Greece. Serial block-face scanning electron microscopy was performed on high pressure frozen and freeze substituted zygospores and 3D reconstructions were generated, allowing a comprehensive insight into the process of zygospore maturation, involving storage compound and organelle rearrangements. Chloroplasts are drastically changed, while young stages contain both parental chloroplasts, the male chloroplasts are aborted and reorganised as ‘secondary vacuoles’ which initially contain plastoglobules and remnants of thylakoid membranes. The originally large pyrenoids and the volume of starch granules is significantly reduced during maturation (young: 8 ± 5 µm³, mature: 0.2 ± 0.2 µm³). In contrast, lipid droplets (LDs) increase significantly in number upon zygospore maturation, while simultaneously getting smaller (young: 21 ± 18 µm³, mature: 0.1 ± 0.2 and 0.5 ± 0.9 µm³). Only in S. mirabilis the LD volume increases (34 ± 29 µm³), occupying ~50% of the zygospore volume. Mature zygospores contain barite crystals as confirmed by Raman spectroscopy with a size of 0.02 - 0.05 µm³. The initially thin zygospore cell wall (~0.5 µm endospore, ~0.8 µm exospore) increases in thickness and develops a distinct, electron dense mesospore, which has a reticulate appearance (~1.4 µm) in Spirogyra sp. from Greece. The exo- and endospore show cellulose microfibrils in a helicoidal pattern. In the denser endospore, pitch angles of the microfibril layers were calculated: ~18 ± 3° in S. mirabilis, ~20 ± 3° in Spirogyra sp. from Austria and ~38 ± 8° in Spirogyra sp. from Greece. Overall this study gives new insights into Spirogyra sp. zygospore development, crucial for survival during dry periods and dispersal of this genus.

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Marine barite morphology as an indicator of biogeochemical conditions within organic matter aggregates

Light,

Martínez-Ruiz,

Norris

2023

Geochimica et Cosmochimica Acta

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Combined in vitro and in vivo investigation of barite microcrystals in Spirogyra (Zygnematophyceae, Charophyta)

Cited by 4 publications

References 53 publications

Biomineralization of ordered dolomite and magnesian calcite by the green alga Spirogyra

Biomineralization of ordered dolomite and magnesian calcite by the green alga Spirogyra

Zygospore development of Spirogyra (Charophyta) investigated by serial block-face scanning electron microscopy and 3D reconstructions

Marine barite morphology as an indicator of biogeochemical conditions within organic matter aggregates

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