Combined isolation of CD34+ progenitor cells and reduction of B cells from peripheral blood by use of immunomagnetic methods

Martín-Henao, G A; Picón, M.; Rueda, Félix; Amill, B; Querol, Sergio; González‐Barca, Eva; Ferrà, Christelle; Grañena, A; Garcı́a, Joan

doi:10.1046/j.1537-2995.2002.00146.x

Cited by 4 publications

(2 citation statements)

References 42 publications

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“…They are based on the utilization of commercially available monoclonal anti-CD34 antibodies and are suitable for the isolation of highly purified CD34 + cells from various hematopoietic sources [ 72 , 73 , 74 , 75 ]. Immunomagnetic beads conjugated with monoclonal anti-CD34 antibodies have been reported, allowing for the efficient isolation of CD34 + progenitor cells from peripheral blood with a degree of purity >90% by use of a magnetic cell selector [ 76 ]. However, CD34 is expressed at low frequency not only in cells from peripheral and umbilical cord blood, but also in cells derived from tissues of non-hematopoietic origin and is therefore considered as general marker for diverse progenitor cells [ 70 , 77 ].…”

Section: Ex Vivo Generation Of Developing Erythrocytesmentioning

confidence: 99%

Valid Presumption of Shiga Toxin-Mediated Damage of Developing Erythrocytes in EHEC-Associated Hemolytic Uremic Syndrome

Detzner

Pohlentz

Müthing

2020

Toxins

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The global emergence of clinical diseases caused by enterohemorrhagic Escherichia coli (EHEC) is an issue of great concern. EHEC release Shiga toxins (Stxs) as their key virulence factors, and investigations on the cell-damaging mechanisms toward target cells are inevitable for the development of novel mitigation strategies. Stx-mediated hemolytic uremic syndrome (HUS), characterized by the triad of microangiopathic hemolytic anemia, thrombocytopenia, and acute renal injury, is the most severe outcome of an EHEC infection. Hemolytic anemia during HUS is defined as the loss of erythrocytes by mechanical disruption when passing through narrowed microvessels. The formation of thrombi in the microvasculature is considered an indirect effect of Stx-mediated injury mainly of the renal microvascular endothelial cells, resulting in obstructions of vessels. In this review, we summarize and discuss recent data providing evidence that HUS-associated hemolytic anemia may arise not only from intravascular rupture of erythrocytes, but also from the extravascular impairment of erythropoiesis, the development of red blood cells in the bone marrow, via direct Stx-mediated damage of maturing erythrocytes, leading to “non-hemolytic” anemia.

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Section: Ex Vivo Generation Of Developing Erythrocytesmentioning

confidence: 99%

Valid Presumption of Shiga Toxin-Mediated Damage of Developing Erythrocytes in EHEC-Associated Hemolytic Uremic Syndrome

Detzner

Pohlentz

Müthing

2020

Toxins

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“…The probability of relapse should drop with less contaminant cells but would likely remain nonzero even with a single cell. However, the contribution of these contaminating tumor cells to relapse of the cancer after treatment is still controversial (15,16). Helping to resolve this controversy by removing all contaminant cancer cells to eliminate this possible source of relapse motivates evaluation of the image cytometry method for purging 100% of the contaminant cells.…”

mentioning

confidence: 99%

Toward complete laser ablation of melanoma contaminant cells in a co‐culture outgrowth model via image cytometry

Shen

Price

2006

Cytometry Pt A

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Background: Contaminant cancer cells in autologous transplant tissue can cause relapse and the rates are unknown. A method capable of removing all contaminant cells with a high probability detected by cytomic analyses would be useful. Neither 100% cell purging nor techniques for measuring the probability of success have been developed. Here, we report a method for removing 100% of the cells under ideal staining conditions and quantify the probability of success. Methods: Laser ablation was combined with previously reported automated microscopy to purge contaminant cells and evaluate 100% ablation in a co-culture model of prestained mouse melanoma cells mixed with mouse NIH-3T3 cells. Melanoma passage efficiency was measured by: (1) micropipetting single cells into microtiter wells and (2) ablating all but one melanoma cell in co-cultures. Results: (74 6 5)% of single melanoma cells pipetted into microtiter plate wells divided at least once. With abla-

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Nanotechnology at the crossroads of stem cell medicine

Jain,

Bhatt,

Gupta

et al. 2025

Biomater. Sci.

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Combined isolation of CD34+ progenitor cells and reduction of B cells from peripheral blood by use of immunomagnetic methods

Abstract: Combined positive and negative magnetic cell selection achieves a high grade of tumor cell reduction with up to 77 percent of the grafts being negative for tumor-specific clonal signal by PCR analysis. This technique preserves an adequate recovery of progenitor cells able to engraft.

Cited by 4 publications

References 42 publications

Valid Presumption of Shiga Toxin-Mediated Damage of Developing Erythrocytes in EHEC-Associated Hemolytic Uremic Syndrome

Valid Presumption of Shiga Toxin-Mediated Damage of Developing Erythrocytes in EHEC-Associated Hemolytic Uremic Syndrome

Toward complete laser ablation of melanoma contaminant cells in a co‐culture outgrowth model via image cytometry

Nanotechnology at the crossroads of stem cell medicine

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