Combined phylogenetic and morphological studies of true morels (Pezizales, Ascomycota) in Cyprus reveal significant diversity, including Morchella arbutiphila and M. disparilis spp. nov.

Loizides, Michael; Bellanger, Jean‐Michel; Clowez, Philippe; Richard, Franck; Moreau, Pierre-Arthur

doi:10.1007/s11557-016-1180-1

Cited by 40 publications

(37 citation statements)

References 41 publications

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“…In this study, we assessed the capability of gene trees from reproductive genes (MAT1-1-1 and MAT1-2-1) and nonreproductive genes (F1 and IGS) to resolve species relationships by comparing genes trees with a species phylogeny framework of the twenty-two species inferred from ITS and EF1-a combined datasets which were derived from multilocus analyses and were previously used in studies of the genus Morchella (Taşkın et al 2010;O'Donnell et al, 2011;Du et al 2012Du et al , 2019bLoizides et al 2016;Baroni et al 2018). One of the findings in this study is the topologies for the reproductive gene trees (mating-type) and nonreproductive genes (F1 and IGS) were in conflict with the species phylogeny framework of the Esculenta Clade.…”

Section: Conflicts Among the Genealogies Of Four Targeted Genes And Smentioning

confidence: 99%

Heterothallism and potential hybridization events inferred for twenty-two yellow morel species

Kang

et al. 2020

IMA Fungus

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Mating-type genes are central to sexual reproduction in ascomycete fungi and result in the establishment of reproductive barriers. Together with hybridization, they both play important roles in the evolution of fungi. Recently, potential hybridization events and MAT genes were separately found in the Elata Clade of Morchella. Herein, we characterized the MAT1-1-1 and MAT1-2-1 genes of twenty-two species in the Esculenta Clade, another main group in the genus Morchella, and proved heterothallism to be the predominant mating strategy among the twenty-two species tested. Ascospores of these species were multi-nuclear and had many mitochondrial nucleoids. The number of ascospore nuclei might be positively related with the species distribution range. Phylogenetic analyses of MAT1-1-1, MAT1-2-1, intergenic spacer (IGS), and partial histone acetyltransferase ELP3 (F1) were performed and compared with the species phylogeny framework derived from the ribosomal internal transcribed spacer region (ITS) and translation elongation factor 1-alpha (EF1-a) to evaluate their species delimitation ability and investigate potential hybridization events. Conflicting topologies among these genes genealogies and the species phylogeny were revealed and hybridization events were detected between several species. Different evolutionary patterns were suggested for MAT genes between the Esculenta and the Elata Clades. Complex evolutionary trajectories of MAT1-1-1, MAT1-2-1, F1 and IGS in the Esculenta Clade were highlighted. These findings contribute to a better understanding of the importance of hybridization and gene transfer in Morchella and especially for the appearance of reproductive modes during its evolutionary process.

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Section: Conflicts Among the Genealogies Of Four Targeted Genes And Smentioning

confidence: 99%

Heterothallism and potential hybridization events inferred for twenty-two yellow morel species

Kang

et al. 2020

IMA Fungus

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“…The genus is distributed worldwide. However, recent molecular phylogenetic studies suggest that the individual species exhibit high continental endemism and provincialism in the Northern Hemisphere (O’Donnell et al 2011), and approximately 20 species have been recorded on more than one continent (Taşkın et al 2010, 2012, 2015; O’Donnell et al 2011; Du et al 2012a; Pildain et al 2014; Richard et al 2015; Loizides et al 2016, 2017; Yatsiuk et al 2016; Loizides 2017). The highest species diversity of true morels is concentrated in Europe and West Asia, East Asia (mainly China), and North America (Du et al 2015; Richard et al 2015).…”

Section: Introductionmentioning

confidence: 99%

How useful is the current species recognition concept for the determination of true morels? Insights from the Czech Republic

Petrželová¹,

Sochor²

2019

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The phylogentic diversity of the genus Morchella has only been sporadically studied in Central Europe. In this study, a molecular taxonomic revision of the Morchella species of the Czech Republic was performed using available fungarium specimens, fresh collections, and axenic cultures. Molecular phylogenetic analyses based on either ITS or five-locus (ITS, LSU, RPB1, RPB2, and EF-1α) sequencing and the application of principles of the genealogical concordance phylogenetic species recognition (GCPSR) have revealed the occurrence of 11 phylogenetic species in the region, but only six of them could be assigned unequivocally to the previously published phylospecies: Mel-3 (M.semilibera), Mel-10 (M.importuna), Mel-19 (M.eohespera), Mes-4 (M.americana), Mes-5 and Mes-8 (M.esculenta). One lineage was identified as a new phylospecies and is designated as Mel-39. Four lineages grouped together with two or more previously published phylospecies: Mel-13/26 (M.deliciosa), Mel-15/16 (M.angusticeps / M.eximioides), Mel-20/34 (M.purpurascens), and Mel-23/24/31/32 (M.pulchella). Our phylogenetic analyses and literature review shed light on the pitfalls of current molecular taxonomy of morels and highlight the ambiguities of present species recognition concepts. The main source of the problems seems to be rooted in the application of different methods (multigene vs single-gene sequencing, phenotypic determination) and approaches (monophyly vs paraphyly, the application or not of GCPSR, degree of differentiation between accepted species, etc.) by various authors for the delimitation of new phylospecies. Therefore, we propose five criteria for distinguishing new phylospecies in the genus Morchella based on molecular data, and recommend a more conservative approach in species delimitation.

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“…Various research studies have been conducted on phylogeny, biogeography, taxonomy, and nomenclature of this genus to identify new species all over the world. In spite of the fact that the primary trait of Morchella species is the high continental endemism and provincialism [1,2], transcontinental species were also discovered [3][4][5][6]. In addition, the inconsistent ecological potential of Morchella species produced symbiotic, endophytic, and saprotrophic abilities [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Statistical Optimization of Culture Conditions for Protein Production by a Newly Isolated Morchella fluvialis

Rahgo

Samadlouie

Mojerlou

et al. 2019

BioMed Research International

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Morchella fungi are considered a good source of protein. The ITS region was used to identify Morchella isolated in the northern region of Iran. The isolated fungus was very similar to Morchella fluvialis. M. fluvialis was first isolated in Iran. Dried biomass of M. fluvialis contained 9% lipids and 50% polysaccharides. Fatty acid profiles of lipids of M. fluvialis are mainly made up of linoleic acid (C18:2) (62%), followed by palmitic acid (C16:0) (12%). Testosterone (TS) was also detected (0.732 ng/dry weight biomass (DWB)) in the hormone profile of this new isolated species. Then, various protein and carbon sources as variable factors were applied to identify the key substrates, which stimulated protein production using the one-factor-at-a-time method. Key substrates (glucose and soybean) were statistically analyzed to determine the optimum content of the protein and DWB accumulation using response surface methods. The highest protein content (38% DWB) was obtained in the medium containing 80 g/l glucose and 40 g/l soybean powder. Total nutritionally indispensable amino acids and conditionally indispensable amino acids constitute 55.7% crude protein. That is to say, these adequate quantities of essential amino acids in the protein of M. fluvialis make it a good and promising source of essential amino acids for human diet.

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Combined phylogenetic and morphological studies of true morels (Pezizales, Ascomycota) in Cyprus reveal significant diversity, including Morchella arbutiphila and M. disparilis spp. nov.

Cited by 40 publications

References 41 publications

Heterothallism and potential hybridization events inferred for twenty-two yellow morel species

Heterothallism and potential hybridization events inferred for twenty-two yellow morel species

How useful is the current species recognition concept for the determination of true morels? Insights from the Czech Republic

Statistical Optimization of Culture Conditions for Protein Production by a Newly Isolated Morchella fluvialis

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