Combined Prebiotic and Microbial Intervention Improves Oral Cholera Vaccination Responses in a Mouse Model of Childhood Undernutrition

Luccia, Blanda Di; Griffin, Nicholas W.; Cheng, Jiye; Guruge, Janaki L.; Byrne, Alexandra E.; Rodionov, Dmitry A.; Leyn, Semen A.; Osterman, Andrei L.; Ahmed, Tahmeed; Colonna, Marco; Barratt, Michael J.; Delahaye, Nicolas; Gordon, Jeffrey I.

doi:10.1016/j.chom.2020.04.008

Cited by 44 publications

(42 citation statements)

References 42 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another report showed that the combination of 2 -fucosyllactose, a dominant oligosaccharide in human milk, long-chain FOS, and short-chain GOS, enhanced the activation of influenza vaccine-specific Th1 cell and B cell activation [139] and increased the levels of IgG1 and IgG2a. Moreover, Luccia et al showed that combined prebiotics (spirulina, amaranth, and flaxseed) and microbial interventions improved the immune responses to CT vaccination in GF mice colonized with the gut microbiota of Bangladeshi nonresponder children [140]. Collectively, these results support the hypothesis that the use of prebiotics may improve vaccine efficacy.…”

Section: Vaccine Efficacy and Gut Microbiotamentioning

confidence: 84%

Understanding Host Immunity and the Gut Microbiota Inspires the New Development of Vaccines and Adjuvants

2021

View full text Add to dashboard Cite

Vaccinations improve the mortality and morbidity rates associated with several infections through the generation of antigen-specific immune responses. Adjuvants are often used together with vaccines to improve immunogenicity. However, the immune responses induced by most on-going vaccines and adjuvants approved for human use vary in individuals; this is a limitation that must be overcome to improve vaccine efficacy. Several reports have indicated that the symbiotic bacteria, particularly the gut microbiota, impact vaccine-mediated antigen-specific immune responses and promote the induction of nonspecific responses via the “training” of innate immune cells. Therefore, the interaction between gut microbiota and innate immune cells should be considered to ensure the optimal immunogenicity of vaccines and adjuvants. In this review, we first introduce the current knowledge on the immunological mechanisms of vaccines and adjuvants. Subsequently, we discuss how the gut microbiota influences immunity and highlight the relationship between gut microbes and trained innate immunity, vaccines, and adjuvants. Understanding these complex interactions will provide insights into novel vaccine approaches centered on the gut microbiota.

show abstract

Section: Vaccine Efficacy and Gut Microbiotamentioning

confidence: 84%

Understanding Host Immunity and the Gut Microbiota Inspires the New Development of Vaccines and Adjuvants

2021

View full text Add to dashboard Cite

show abstract

“…An important caveat underlying our findings is that GF mice lack commensal gut microbes and could have altered immune responses to immunization, especially considering recent studies that have highlighted how V. cholerae-microbiota interactions can influence colonization, disease, and development of anti-V. cholerae immunity [48][49][50][51][52] . However, the strong association of VAT induction with protection and superior efficacy of live over inactivated vaccine strains in this model, and our similar findings of OCV function in mice with transiently disrupted microbiomes reinforces the idea that the GF mouse live OCV model holds substantial translational promise 36,37 .…”

Section: Discussionmentioning

confidence: 99%

Dissecting serotype-specific contributions to live oral cholera vaccine efficacy

Sit

Fakoya

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

The O1 serogroup of Vibrio cholerae causes pandemic cholera and is divided into Ogawa and Inaba serotypes. The O-antigen is the immunodominant antigen of V. cholerae, and the two serotypes, which differ by the presence or absence of a terminally methylated O-antigen, likely influence development of immunity to cholera and oral cholera vaccines (OCVs). However, there is no consensus regarding the relative immunological potency of each serotype, in part because previous studies relied on genetically heterogenous strains. Here, we engineered matched serotype variants of a live OCV candidate, HaitiV, and used a germ-free mouse model to evaluate the immunogenicity and protective efficacy of each vaccine serotype. By combining vibriocidal antibody quantification with single and mixed strain infection assays, we found that all three HaitiV variants - InabaV, OgawaV, and HikoV (bivalent Inaba/Ogawa) - were immunogenic and protective, suggesting the impact of O1 serotype variation on OCV function may be minimal. The potency of OCVs was found to be challenge strain-dependent, emphasizing the importance of appropriate strain selection for cholera challenge studies. Our findings and experimental approaches will be valuable for guiding the development of live OCVs and oral vaccines for additional pathogens.

show abstract

“…In gnotobiotic mice model, improved CT-IgA response was observed after colonizing with diet-specific microbiome (Di Luccia et al, 2020 ). In several studies, it was shown that the commensal microbes act as a barrier against enteric pathogens.…”

Section: Influence Of Microbiome On V Cholerae Pamentioning

confidence: 99%

Virulence Regulation and Innate Host Response in the Pathogenicity of Vibrio cholerae

Ramamurthy

Nandy

Mukhopadhyay

et al. 2020

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

The human pathogen Vibrio cholerae is the causative agent of severe diarrheal disease known as cholera. Of the more than 200 "O" serogroups of this pathogen, O1 and O139 cause cholera outbreaks and epidemics. The rest of the serogroups, collectively known as non-O1/non-O139 cause sporadic moderate or mild diarrhea and also systemic infections. Pathogenic V. cholerae circulates between nutrient-rich human gut and nutrient-deprived aquatic environment. As an autochthonous bacterium in the environment and as a human pathogen, V. cholerae maintains its survival and proliferation in these two niches. Growth in the gastrointestinal tract involves expression of several genes that provide bacterial resistance against host factors. An intricate regulatory program involving extracellular signaling inputs is also controlling this function. On the other hand, the ability to store carbon as glycogen facilitates bacterial fitness in the aquatic environment. To initiate the infection, V. cholerae must colonize the small intestine after successfully passing through the acid barrier in the stomach and survive in the presence of bile and antimicrobial peptides in the intestinal lumen and mucus, respectively. In V. cholerae, virulence is a multilocus phenomenon with a large functionally associated network. More than 200 proteins have been identified that are functionally linked to the virulence-associated genes of the pathogen. Several of these genes have a role to play in virulence and/or in functions that have importance in the human host or the environment. A total of 524 genes are differentially expressed in classical and El Tor strains, the two biotypes of V. cholerae serogroup O1. Within the host, many immune and biological factors are able to induce genes that are responsible for survival, colonization, and virulence. The innate host immune response to V. cholerae infection includes activation of several immune protein complexes, receptor-mediated signaling pathways, and other bactericidal proteins. This article presents an overview of regulation of important virulence factors in V. cholerae and host response in the context of pathogenesis.

show abstract

Combined Prebiotic and Microbial Intervention Improves Oral Cholera Vaccination Responses in a Mouse Model of Childhood Undernutrition

Cited by 44 publications

References 42 publications

Understanding Host Immunity and the Gut Microbiota Inspires the New Development of Vaccines and Adjuvants

Understanding Host Immunity and the Gut Microbiota Inspires the New Development of Vaccines and Adjuvants

Dissecting serotype-specific contributions to live oral cholera vaccine efficacy

Virulence Regulation and Innate Host Response in the Pathogenicity of Vibrio cholerae

Contact Info

Product

Resources

About