Combined ribavirin treatment and cryotherapy for efficient Potato virus M and Potato virus S eradication in potato (Solanum tuberosum L.) in vitro shoots

Кушнаренко, С. В.; Ромаданова, Н.В.; Aralbayeva, Moldir; Zholamanova, Saule; Alexandrova, Alena; Карпова, О.В.

doi:10.1007/s11627-017-9839-0

Cited by 27 publications

(28 citation statements)

References 12 publications

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“…And it is not a time consuming technique as shown by the previous report (Schäfer-Menuhr et al 1996). However, the method was found effective for elimination of PVM but ineffective for a mixed infection of PVS and PVM (Kushnarenko et al 2017). Some cultivars are co-infected by a mixture of viruses such as PVM + PVS + PVY, PVM + PVYor PVS + PVY, and cryotherapy was found inefficient for PVS and PVM eradication but can remove PVY.…”

Section: Cryotherapy Alone or Combined With Other Techniques To Eradimentioning

confidence: 90%

“…Therefore, it is recommended to combine two therapies to improve the concentration of virus elimination while increasing the regeneration capacity of plantlets. Furthermore, simultaneous use of antiviral agent and temperature, as well as other combinations of viral therapy for potato plants have been reported to ensure effective virus elimination (Chahardehi et al 2016;Kushnarenko et al 2017). A significant difference of (P < 0.05) has been found between thermotherapy with and without ribavirin for potato plants (Cordeiro 2003), and the same reports showed virus concentration to be potentially eliminated when thermotherapy is combined with chemotherapy with ribavirin.…”

Section: Thermotherapy Technique For In Vitro Potato Virus Eliminationmentioning

confidence: 99%

“…The effectiveness of viral elimination by antiviral agents is proportional to their concentrations and to their form of application (Cordeiro 2003). (Hu et al 2015;Singh 2015;Chahardehi et al 2016;Kushnarenko et al 2017). Ribavirin, a guanosine synthetic derivative has been synthesized in 1972, and reports have proved it as one of the most promising antiviral drugs (Sidwell et al 1972;Yang et al 2013;Singh 2015).…”

Section: Chemotherapy Technique To Eradicate Potato Virusesmentioning

confidence: 99%

“…However, chemotherapy was found inefficient for PVYelimination. Therefore, it was suggested that cryotherapy combined with chemotherapy is much more efficient for coinfected cultivars especially when PVS and PVM are included Yang et al 2013;Kushnarenko et al 2017).…”

Section: Cryotherapy Alone or Combined With Other Techniques To Eradimentioning

confidence: 99%

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Major In Vitro Techniques for Potato Virus Elimination and Post Eradication Detection Methods. A Review

2019

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Potato is an economically important agro-industrial crop that is conventionally propagated, however; its potential transmission of viruses through seed tubers from generation to generation is a major limitation of potato yield production. In order to produce potato virus-free and sufficient amount of potato seed tubers, several approaches of in vitro methods for virus elimination have been developed. Meristem culture has been used alone or combined with techniques such as thermotherapy, electrotherapy, cryotherapy and chemotherapy as the best alternative method for treating potato infected by viruses. Recent literature has shown that to eliminate potato virus significantly depends upon the potato cultivar, antiviral agents, type of virus, the duration of heat treatment. Appropriate duration for efficiency elimination is still under investigation. Viral elimination rate can be detected through serological methods such as enzyme-linked immunosorbent assay (ELISA) and molecular biology technique such as real time reverse transcriptase polymerase chain reaction (real time RT-PCR) that are used for pre and post elimination virus detection to evaluate the efficiency and the accuracy of virus elimination method. The purpose of this review is to highlight virus elimination methods in potato and recommending the most effective tool for virus detection in order to ensure the production of potato plantlet free of viruses. Resumen La papa es un cultivo de importancia económica agro-industrial que se propaga de manera convencional, no obstante, la potencial transmisión de virus a través del tubérculo-semilla de generación en generación es una limitación mayor en la producción del rendimiento. Con el fin de producir papa libre de virus y suficiente cantidad de tubérculo-semilla de papa, se han desarrollado varias estrategias de métodos in vitro para la eliminación de los virus. El cultivo de meristemos se ha usado solo o combinado con técnicas tales como termoterapia, electroterapia, crioterapia y quimioterapia, como el mejor método alternativo para tratar papa infectada por virus. La literatura reciente ha demostrado que para eliminar virus significativamente se depende de la variedad de papa, agentes antivirales, tipo de virus, la duración del tratamiento térmico. Aun esta bajo investigación la duración apropiada para la eliminación eficiente. El nivel de la eliminación del virus puede detectarse por métodos serológicos, tales como el inmunoensayo con enzimas conjugadas (ELISA) y técnica de biología molecular, como la reverso-transcripción de reacción en cadena de la polimerasa en tiempo real (RT-PCR) que se usan para la detección del virus pre y post eliminación para evaluar la eficiencia y la precisión del método de eliminación del virus. El propósito de esta revisión es resaltar los métodos de eliminación de virus en papa y en la recomendación de la herramienta más efectiva para la detección de virus para asegurar la producción de plántulas de papa libres de virus.

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Section: Cryotherapy Alone or Combined With Other Techniques To Eradimentioning

confidence: 90%

Section: Thermotherapy Technique For In Vitro Potato Virus Eliminationmentioning

confidence: 99%

Section: Chemotherapy Technique To Eradicate Potato Virusesmentioning

confidence: 99%

Section: Cryotherapy Alone or Combined With Other Techniques To Eradimentioning

confidence: 99%

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Major In Vitro Techniques for Potato Virus Elimination and Post Eradication Detection Methods. A Review

2019

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“…A list of cultures in the collection was included in the Botanic Gardens Conservation International (BGCI) database (Romadanova et al 2016a). Cryopreservation is not only used for the long-term preservation of the plant genetic diversity in Kazakhstan, but also for the recovery of crops from phytopathogens (Kushnarenko et al 2017;Romadanova et al 2016b).…”

Section: Conservation and Study Of Genetic Resources Of The Kazakhstamentioning

confidence: 99%

Current status and prospects of plant biotechnology in Kazakhstan

Zhambakin

Zhapar

2020

Plant Biotechnol Rep

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Biotechnological methods are becoming an integral part of biological research. This review presents some of the most significant scientific results of Kazakhstan biologists in the field of plant biotechnology over the past 10 years. One of the recent important areas of application of biotechnological methods is the conservation and study of plant genetic resources and bioremediation. Studies on the flora lead to the identification of new sources of previously unknown biologically active materials, especially among wild plants growing in Kazakhstan. In addition, various biotechnological approaches are used to increase the efficiency of breeding practices for the production of new crop varieties.

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