Combined subtotal pancreatectomy with selective streptozotocin infusion — a model for the induction of insulin deficiency in dogs

Tschöepe, Diethelm; Job, F. P.; Huebinger, A.; Freytag, G; Torsello, Giovanni; Peter, B; Gries, F. A.

doi:10.1007/bf01851264

Cited by 7 publications

(3 citation statements)

References 16 publications

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“…In addition, we have extended our search by applying another sub filter, "Diabetes." Interestingly, we have found only 56 ). The reviews, systematic review, meta-analysis, and books/documents were excluded from the analysis.…”

Section: Organ-on-chip Models In Diabetic Researchmentioning

confidence: 55%

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Animal Models in Diabetic Research—History, Presence, and Future Perspectives

Pandey,

Chmelir,

Chottova Dvorakova

2023

Biomedicines

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Diabetes mellitus (DM) is a very serious disease, the incidence of which has been increasing worldwide. The beginning of diabetic research can be traced back to the 17th century. Since then, animals have been experimented on for diabetic research. However, the greatest development of diabetes research occurred in the second half of the last century, along with the development of laboratory techniques. Information obtained by monitoring patients and animal models led to the finding that there are several types of DM that differ significantly from each other in the causes of the onset and course of the disease. Through different types of animal models, researchers have studied the pathophysiology of all types of diabetic conditions and discovered suitable methods for therapy. Interestingly, despite the unquestionable success in understanding DM through animal models, we did not fully succeed in transferring the data obtained from animal models to human clinical research. On the contrary, we have observed that the chances of drug failure in human clinical trials are very high. In this review, we will summarize the history and presence of animal models in the research of DM over the last hundred years. Furthermore, we have summarized the new methodological approaches, such as “organ-on-chip,” that have the potential to screen the newly discovered drugs for human clinical trials and advance the level of knowledge about diabetes, as well as its therapy, towards a personalized approach.

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Section: Organ-on-chip Models In Diabetic Researchmentioning

confidence: 55%

“…The advantage of this application is to use a lower amount of each of these substances and, thus, prevent damage to other organs [54,55]. Another option for safely inducing T1DM in a dog is a partial pancreatotomy with the application of a low dose of STZ [56]. In the same way, diabetes can be safely induced in a monkey [57].…”

Section: Chemical Modelsmentioning

confidence: 99%

Animal Models in Diabetic Research—History, Presence, and Future Perspectives

Pandey,

Chmelir,

Chottova Dvorakova

2023

Biomedicines

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“…As we all know, DM is a research hotspot in the field of scientific research. At present, there are many studies on small diabetic animal models such as rats, rabbits, and hamsters, and the modeling technology has been relatively mature, but there are few studies on large diabetic animal models such as dogs [7][8][9]. The small diabetic animal models are widely used in the research and development of diabetes drugs or technologies, but with the limitations of body size and lifespan, it has limited reference significance for the clinical treatment of diabetes [10].…”

Section: Discussionmentioning

confidence: 99%

Repeated Low‐Dose Streptozotocin and Alloxan Induced Long‐Term and Stable Type 1 Diabetes Model in Beagle Dogs

Han

Sun

Xie

et al. 2022

BioMed Research International

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Type 1 diabetes mellitus (T1DM) is a chronic disease represented by insulin-causing pancreatic β-cell disruption and hyperglycemia. Therefore, it is necessary to establish a variety of animal models of diabetes to study the pathogenesis and pathophysiology of it. However, there are few reports on the use of beagle dogs to establish an animal model of type 1 diabetes. This study aimed to explore a simple and feasible modeling method to establish a long-term and stable type 1 diabetes model in beagle dogs. Forty adult beagle dogs were randomly divided into control group and model group. After 24 h of fasting, streptozotocin (20 mg/kg) and alloxan (20 mg/kg) were injected through the cephalic vein. The second intravenous injection was given on the 4th day after the first injection. Insulin release testing was performed on the 7th day after the last intravenous injection. Fasting blood glucose and body weight were recorded monthly. Four months after the last injection, the serum fructosamine content and the ratio of glycated hemoglobin were detected. Then, the pancreatic tissue was harvested for histopathological examination. The results showed that the level of fasting blood glucose of the 16 dogs in the model group was consistently higher than 11.1 mmol/L for 4 consecutive months. Moreover, compared with the control group, the insulin release curve of the model group was flat with no increase. The body weight of the model group was significantly reduced, and the ratios of blood glucose, fructosamine, and glycosylated hemoglobin were significantly higher than those in the control group. Meanwhile, histopathological examination of the pancreas showed that the islet beta cells appeared to have vacuoles or even necrosis. In the model group, pancreatic β-cells were damaged and insulin release was reduced. These results suggest that the above modeling methods can induce long-term and stable type 1 diabetes models in beagle dogs.

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An improved method of 90% pancreatectomy using a low dose of streptozotocin at the pancreaticoduodenal artery results in a rapid diabetic stage in dogs

et al. 2005

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An improved method of inducing diabetes in dogs was developed. This method included 90% pancreatectomy, 2 mg/kg streptozotocin (STZ) perfused into pancreaticoduodenal artery, and the fixation suture of the duodenum to the costo-abdominal wall. Vasopressin injection administered to the animals before surgery reduced bleeding. All dogs used in this procedure survived and became diabetic. One month after the procedure the pancreatic islets were reduced in volume and the number compared with pancreas tissue obtained during the surgery. Acinar tissue remained with a normal histology, and exocrine function maintained the physiological parameters, except for a soft faecal consistency. We conclude that this procedure is effective in inducing experimental diabetes in dogs.

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Combined subtotal pancreatectomy with selective streptozotocin infusion — a model for the induction of insulin deficiency in dogs

Cited by 7 publications

References 16 publications

Animal Models in Diabetic Research—History, Presence, and Future Perspectives

Animal Models in Diabetic Research—History, Presence, and Future Perspectives

Repeated Low‐Dose Streptozotocin and Alloxan Induced Long‐Term and Stable Type 1 Diabetes Model in Beagle Dogs

An improved method of 90% pancreatectomy using a low dose of streptozotocin at the pancreaticoduodenal artery results in a rapid diabetic stage in dogs

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