Combined tetraphenylethylene fluorogens with positive charge for imaging capsule-covered pathogens

Ai, Wenting; Yang, Zhiqiang; Ma, Yan; Han, Xiaomin; Chen, Yuzhi; Zhu, Kui; Wang, Zhuo

doi:10.1039/d0an00349b

Cited by 7 publications

(7 citation statements)

References 30 publications

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“…This result could be attributed to aggregation formation of P1 and P4 molecules in water solvent, which inhibited non-radiative relaxation channels and reduced energy loss, thus enhancing fluorescence. Furthermore, considering that P2, P3, P5, and P6 with dual positive charge sites had good hydrophilicity and very weak fluorescence emission in water, so water and glycerol were selected to verify their AIE features through viscosity changes. , As shown in Figures B and S41B,D,E, fluorescence intensity of P2–P3 at 610 nm and P5–P6 at 640 nm gradually enhanced as the glycerol volume fraction ( f g ) increased in H 2 O-glycerol mixture solution. The maximum fluorescence intensity of P2, P3, P5, and P6 increased by approximately 20-fold, 20-fold, 17-fold, and 20-fold in 99% glycerol compared to that in pure water, respectively.…”

Section: Results and Discussionmentioning

confidence: 99%

Mitochondrial-Targeted AIE-Active Fluorescent Probe Based on Tetraphenylethylene Fluorophore with Dual Positive Charge Recognition Sites for Monitoring ATP in Cells

et al. 2023

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Adenosine triphosphate (ATP), as an important intracellular energy currency produced in mitochondria, is closely related to various diseases in living organisms. Currently, the biological application of AIE fluorophore as a fluorescent probe for ATP detection in mitochondria is rarely reported. Herein, D−π−A and D−A structure-based tetraphenylethylene (TPE) fluorophores were employed to synthesize six different ATP probes (P1−P6), and the phenylboronic acid groups and dual positive charge sites of probes could interact with the vicinal diol of ribose and negatively charged triphosphate structure of ATP, respectively. However, P1 and P4 with a boronic acid group and a positive charge site had poor selectivity for ATP detection. In contrast, P2, P3, P5, and P6 with dual positive charge sites exhibited better selectivity than P1 and P4. In particular, P2 had more advantages of high sensitivity, selectivity, and good time stability for ATP detection than P3, P5, and P6, which was ascribed to its D−π−A structure, linker 1 (1,4-bis(bromomethyl)benzene), and dual positive charge recognition sites. Then, P2 was employed to detect ATP, and it exhibited a low detection limit of 3.62 μM. Moreover, P2 showed utility in the monitoring of mitochondrial ATP level fluctuations.

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Section: Results and Discussionmentioning

confidence: 99%

Mitochondrial-Targeted AIE-Active Fluorescent Probe Based on Tetraphenylethylene Fluorophore with Dual Positive Charge Recognition Sites for Monitoring ATP in Cells

et al. 2023

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“…Capsular polysaccharide mediates anti-phagocytic activities by reducing the C3b deposition on bacterial surface ( 23 , 24 ). Therefore, we investigated the capsular difference in LM, MM, and HM using the zwitterionic TPE-Pn ++ (with strong membrane-penetrating capability) and monocharged TPE-N + (unable to stain bacteria with capsular) ( 25 ). Results indicate that the HM strain carries a thick capsule ( Figure 5A and Supplementary Figure 7 ).…”

Section: Resultsmentioning

confidence: 99%

“…Bacterial imaging assay was performed as described previously ( 25 ). Specifically, fresh prepared bacterial cultures were washed and resuspended with 200 μL PBS.…”

Section: Methodsmentioning

confidence: 99%

Mucoid Acinetobacter baumannii enhances anti-phagocytosis through reducing C3b deposition

Gong

Zhao

et al. 2022

Front. Med.

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Background: Multidrug resistant (MDR) Acinetobacter baumannii causes serious infections in intensive care units and is hard to be eradicated by antibiotics. Many A. baumannii isolates are identified as the mucoid type recently, but the biological characteristics of mucoid A. baumannii and their interactions with host cells remains unclear.Methods: The mucoid phenotype, antimicrobial susceptibility, biofilmforming ability, acid resistance ability, peroxide tolerance, and in vivo toxicity of clinical ICUs derived A. baumannii isolates were first investigated. Secondly, the phagocytic resistance and invasive capacity of A. baumannii isolates to macrophages (MH-S, RAW264.7) and epithelial cells (A549) were analyzed. Furthermore, the abundance of C3b (complement factor C3 degradation product) deposition on the surface of A. baumannii was investigated. Last, the relationship between C3b deposition and the abundance of capsule in A. baumannii isolates were analyzed.Results: These A. baumannii strains showed different mucoid phenotypes including hyper mucoid (HM), medium mucoid (MM), and low mucoid (LM). All tested strains were MDR with high tolerance to either acid or hydrogen peroxide exposure. Notably, these mucoid strains showed the increase of mortality in the Galleria mellonella infection models. Besides, the HM strain exhibited less biofilm abundance, higher molecular weight (MW) of capsule, and greater anti-phagocytic activity to macrophages than the LM strain. Together with the increased abundance of capsule, high expression of tuf gene (associated with the hydrolysis of C3b), the HM strain effectively inhibits C3b deposition on bacterial surface, resulting in the low-opsonization phenotype.

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“…3(e)) and have been widely used in cancer diagnosis/therapy, bacterial/cellular imaging and the detection of oxidative stress biomarkers. 62–65…”

Section: Design Of Fluorescent Probes For Ronss Imagingmentioning

confidence: 99%

“…3(e)) and have been widely used in cancer diagnosis/therapy, bacterial/cellular imaging and the detection of oxidative stress biomarkers. [62][63][64][65] 2.1.5 Coumarin dyes. Coumarins, with the structure of 2H-chromen-2-one motif exhibit good biocompatibility, excellent photophysical properties, easy structural modification and regulation of optical properties (Fig.…”

Section: Fluorophoresmentioning

confidence: 99%

Recent progress in the development of fluorescent probes for imaging pathological oxidative stress

et al. 2023

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Combined tetraphenylethylene fluorogens with positive charge for imaging capsule-covered pathogens

Abstract: The capsular pathogens can cause serious infectious diseases, and is highly pathogenic to humans. Herein, we develop four positive charged tetraphenylethylene derivatives (PC-TPEgens). The combination of PC-TPEgens are applied to...

Cited by 7 publications

References 30 publications

Mitochondrial-Targeted AIE-Active Fluorescent Probe Based on Tetraphenylethylene Fluorophore with Dual Positive Charge Recognition Sites for Monitoring ATP in Cells

Mitochondrial-Targeted AIE-Active Fluorescent Probe Based on Tetraphenylethylene Fluorophore with Dual Positive Charge Recognition Sites for Monitoring ATP in Cells

Mucoid Acinetobacter baumannii enhances anti-phagocytosis through reducing C3b deposition

Recent progress in the development of fluorescent probes for imaging pathological oxidative stress

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