Combined therapy of adipose-derived stem cells and photobiomodulation on accelerated bone healing of a critical size defect in an osteoporotic rat model

Asgari, Mehrdad; Gazor, Rouhollah; Abdollahifar, Mohammad‐Amin; Fathabady, Fatemeh Fadaei; Zare, Fatemeh; Norouzian, Mohsen; Amini, Abdollah; Khosravipour, Armin; Kiani, Pejman; Atashgah, Rahimeh Bairami; Rezaei, Fatemeh; Ghoreishi, Seyed Kamran; Chien, Sufan; Hamblin, Michael R.; Bayat, Mohammad

doi:10.1016/j.bbrc.2020.06.023

Cited by 16 publications

(14 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…And (6) S + PBM + ASCs group. 41 At the fourth (anabolic phase of bone healing) and eighth week (catabolic phase of bone healing) post-operation, six rats from each group were euthanized and the left and right distal femurs were isolated and CSFDs were examined by the cone beam computed tomography (CBCT) scan and histology respectively. Groups 2-6 were considered as treatment groups.…”

Section: Animals and Study Designmentioning

confidence: 99%

“…After cutting the bones into the small pieces, a series of physical and chemical procedures were performed to remove mineral content, blood, fat and cells. 41…”

Section: Preparation Of the Demineralized Bone Matrix Scaffoldmentioning

confidence: 99%

“…The flow cytometry analysis was performed to characterize the hASCs based on previously reported MSC markers. 41,42…”

Section: Isolation Expansion and Immunophenotyping Of Human Ascsmentioning

confidence: 99%

“…Finally, each cube of the DBM (containing hASCs) was implanted into the CSFD of the rats (groups 5, and 6). 41…”

Section: Seeding Hascs On Dbm Scaffoldsmentioning

confidence: 99%

“…In the S + ASCs and S + PBM + ASCs groups, the bone defect was filled with the hybrid hASCs -DBM scaffold. 41 Photobiomodulation CSFD in groups 3 and 6 were immediately subjected to PBM (MUSTANG 2000, LO7 probe; Technica Co, Moscow, Russia) after surgery. PBM therapy continued 3 days a week for 4 and 8 weeks on the basis of the time of the euthanization.…”

Section: Csfd Model and Dbm Scaffold Transplantationmentioning

confidence: 99%

See 4 more Smart Citations

Simultaneous Treatment of Photobiomodulation and Demineralized Bone Matrix With Adipose-Derived Stem Cells Improve Bone Healing in an osteoporotic bone defect

et al. 2021

Self Cite

View full text Add to dashboard Cite

Introduction: The ability of simultaneous treatment of critical-sized femoral defects (CSFDs) with photobiomodulation (PBM) and demineralized bone matrix (DBM) with or without seeded adipose-derived stem cells (ASCs) to induce bone reconstruction in ovariectomized induced osteoporotic (OVX) rats was investigated. Methods: The OVX rats with CSFD were arbitrarily separated into 6 groups: control, scaffold (S, DBM), S + PBM, S + alendronate (ALN), S + ASCs, and S + PBM + ASCs. Each group was assessed by cone beam computed tomography (CBCT) and histological examinations. Results: In the fourth week, CBCT and histological analyses revealed that the largest volume of new bone formed in the S + PBM and S + PBM + ASC groups. The S + PBM treatment relative to the S and S + ALN treatments remarkably reduced the CSFD (Mann-Whitney test, P = 0.009 and P = 0.01). Furthermore, S + PBM + ASCs treatment compared to the S and S + ALN treatments significantly decreased CSFD (Mann Whitney test, P = 0.01). In the eighth week, CBCT analysis showed that extremely enhanced bone regeneration occurred in the CSFD of the S + PBM group. Moreover, the CSFD in the S + PBM group was substantially smaller than S, S + ALN and S + ASCs groups (Mann Whitney test, P = 0.01, P = 0.02 and P = 0.009). Histological observations showed more new bone formation in the treated CSFD of S + PBM + ASCs and S + PBM groups. Conclusion: The PBM plus DBM with or without ASCs significantly enhanced bone healing in the CSFD in OVX rats compared to control, DBM alone, and ALN plus DBM groups. The PBM plus DBM with or without ASCs significantly decreased the CSFD area compared to either the solo DBM or ALN plus DBM treatments.

show abstract

Section: Animals and Study Designmentioning

confidence: 99%

“…After cutting the bones into the small pieces, a series of physical and chemical procedures were performed to remove mineral content, blood, fat and cells. 41…”

Section: Preparation Of the Demineralized Bone Matrix Scaffoldmentioning

confidence: 99%

“…The flow cytometry analysis was performed to characterize the hASCs based on previously reported MSC markers. 41,42…”

Section: Isolation Expansion and Immunophenotyping Of Human Ascsmentioning

confidence: 99%

“…Finally, each cube of the DBM (containing hASCs) was implanted into the CSFD of the rats (groups 5, and 6). 41…”

Section: Seeding Hascs On Dbm Scaffoldsmentioning

confidence: 99%

Section: Csfd Model and Dbm Scaffold Transplantationmentioning

confidence: 99%

See 3 more Smart Citations

Simultaneous Treatment of Photobiomodulation and Demineralized Bone Matrix With Adipose-Derived Stem Cells Improve Bone Healing in an osteoporotic bone defect

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

Photobiomodulation with polychromatic light (600–1200 nm) improves fat graft survival by increasing adipocyte viability, neovascularization, and reducing inflammation in a rat model

et al. 2021

View full text Add to dashboard Cite

Objectives Unpredictability with the final volume and viability of the graft are the major concerns in fat grafting. An experimental study was conducted to increase graft retention using photobiomodulation (PBM) with polychromatic light in near‐infrared region (600–1200 nm) by utilizing its stimulatory effects on angiogenesis, neovascularization, adipocyte viability, and anti‐inflammatory properties. Methods A total of 24 rats were divided into four groups (n = 6) according to the applied polychromatic light protocol to the recipient site (none, before fat transfer, after fat transfer, and combined). In all groups, inguinal fat pad was excised, measured for volume and weight, and transferred to the dorsum of the rat. At the end of the experiment, fat grafts were harvested from the recipient site for volume and weight measurements, histological, and immunohistochemical evaluation. Results Intergroup comparison revealed that fat graft retention regarding weight and volume, was significantly superior in Group IV (p = 0.049 and p = 0.043, respectively), which polychromatic light was applied both before and after transfer of the graft. Hematoxylin–eosin and Masson's trichrome stained sections showed absence of necrosis, fibrosis, inflammation, cyst formation, and increased vascularization of both inner and outer zones of the grafts in Group IV. Also, immunohistochemical staining scores for perilipin (indicator for adipocyte viability), CD31 and VEGF (indicators for angiogenesis and neovascularization) were significantly higher (p < 0.001). Ki67 scores were significantly lower in this group because of anti‐inflammatory environment (p < 0.001). Conclusions Application of PBM to the recipient site before and after fat transfer improved outcomes in rats at 56 day after fat grafting by means of volume retention, increased neovascularization and adipocyte viability and reduced necrosis, fibrosis and inflammation.

show abstract

Evaluation of the effects of preconditioned human stem cells plus a scaffold and photobiomodulation administration on stereological parameters and gene expression levels in a critical size bone defect in rats

et al. 2022

View full text Add to dashboard Cite

Combined therapy of adipose-derived stem cells and photobiomodulation on accelerated bone healing of a critical size defect in an osteoporotic rat model

Cited by 16 publications

References 31 publications

Simultaneous Treatment of Photobiomodulation and Demineralized Bone Matrix With Adipose-Derived Stem Cells Improve Bone Healing in an osteoporotic bone defect

Simultaneous Treatment of Photobiomodulation and Demineralized Bone Matrix With Adipose-Derived Stem Cells Improve Bone Healing in an osteoporotic bone defect

Photobiomodulation with polychromatic light (600–1200 nm) improves fat graft survival by increasing adipocyte viability, neovascularization, and reducing inflammation in a rat model

Evaluation of the effects of preconditioned human stem cells plus a scaffold and photobiomodulation administration on stereological parameters and gene expression levels in a critical size bone defect in rats

Contact Info

Product

Resources

About