Evaluation of the effects of preconditioned human stem cells plus a scaffold and photobiomodulation administration on stereological parameters and gene expression levels in a critical size bone defect in rats

Khosravipour, Armin; Amini, Abdollah; Farahani, Reza Masteri; Mostafavinia, Atarodalsadat; Asgari, Mehrdad; Rezaei, Fatemehalsadat; Abrahamse, Heidi; Chien, Sufan; Bayat, Mohammad

doi:10.1007/s10103-022-03509-z

Cited by 7 publications

(2 citation statements)

References 47 publications

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“…Here, the defects continued to the central part of the medullary channel. After creating CSFD, we implanted the hDBM scaffolds into the produced CSFDs of all rats [18,17].…”

Section: Establishment Of Rat Csfd Modelmentioning

confidence: 99%

MicroRNAs-26 and related osteogenic target genes could play pivotal roles in Photobiomodulation and adipose-derived stem cells-based healing of critical size foot defects in the rat model

Mohebbi

Siasi

Khosravipour

et al. 2022

Preprint

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Background Photobiomodulation therapy (PBMT) and human adipose-derived stem cells (hADSs) treatment are relatively invasive methods for treating bone defects. Specific and oriented cellular and molecular functions can be induced by applying an appropriate type of PBMT and ADSs. In this study, we aimed to explore the role of MicroRNAs pathways in the PBMT, hADS, and PBM+hADS based on promoting critical size fracture healing.MethodsA critical size femoral defect (CSFD) is induced in the right and left femoral bones of 24 rats. Then a human demineralized bone matrix scaffold (hDBMS) was engrafted into the CSFDs of all rats. Experiments are performed on 4 groups (12 rats per group): (1) Control (hDBMS); (2) hDBMS+hADS, hADS was engrafted into CSFDs; (3) hDBMS+PBM, the CSFD was exposed to PBM(810 nm wavelength, 1.2 J/cm2 energy density); (4) hDBMS+(hADS+PBM), hADSs were implanted into CSFD then were exposed to PBM. At 42 days after CSFD induction, the rats were killed and, the left and right CSFDs were removed for the mechanical compression tests, and molecular and cellular studies, respectively.ResultsThe results indicate that miRNA-26a, BMP, SMAD, RUNX, and OSTREX were more expressed in the treated groups than in the control group. Furthermore, the biomechanical and histological properties of CSFDs in treated groups were better than the control group. The correlation tests revealed a positive relationship between microRNA and improving biomechanical and cellular parameters of CSFDs in the rat model.ConclusionWe concluded that the MicroRNA-26 signaling pathway plays a significant role in the hADS, PBM, and hADS+PBM-based healing of CSFDs in rats.

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“…Here, the defects continued to the central part of the medullary channel. After creating CSFD, we implanted the hDBM scaffolds into the produced CSFDs of all rats [18,17].…”

Section: Establishment Of Rat Csfd Modelmentioning

confidence: 99%

MicroRNAs-26 and related osteogenic target genes could play pivotal roles in Photobiomodulation and adipose-derived stem cells-based healing of critical size foot defects in the rat model

Mohebbi

Siasi

Khosravipour

et al. 2022

Preprint

Self Cite

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“…PBM therapy restores the osteogenic capacity of MSCs derived from diabetic rats and enhances bone formation in rat dental alveolus filled with hydroxyapatite [ 29 , 30 ]. Additionally, PBM increases bone repair in critical size defects treated with either MSCs derived from dental pulp combined with hydrogel or MSCs from adipose tissue combined with decellularized bone matrix [ 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stem Cells Combined with a P(VDF-TrFE)/BaTiO3 Scaffold and Photobiomodulation Therapy Enhance Bone Repair in Rat Calvarial Defects

Adolpho

Ribeiro

Freitas

et al. 2023

JFB

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Background: Tissue engineering and cell therapy have been the focus of investigations on how to treat challenging bone defects. This study aimed to produce and characterize a P(VDF-TrFE)/BaTiO3 scaffold and evaluate the effect of mesenchymal stem cells (MSCs) combined with this scaffold and photobiomodulation (PBM) on bone repair. Methods and results: P(VDF-TrFE)/BaTiO3 was synthesized using an electrospinning technique and presented physical and chemical properties suitable for bone tissue engineering. This scaffold was implanted in rat calvarial defects (unilateral, 5 mm in diameter) and, 2 weeks post-implantation, MSCs were locally injected into these defects (n = 12/group). Photobiomodulation was then applied immediately, and again 48 and 96 h post-injection. The μCT and histological analyses showed an increment in bone formation, which exhibited a positive correlation with the treatments combined with the scaffold, with MSCs and PBM inducing more bone repair, followed by the scaffold combined with PBM, the scaffold combined with MSCs, and finally the scaffold alone (ANOVA, p ≤ 0.05). Conclusions: The P(VDF-TrFE)/BaTiO3 scaffold acted synergistically with MSCs and PBM to induce bone repair in rat calvarial defects. These findings emphasize the need to combine a range of techniques to regenerate large bone defects and provide avenues for further investigations on innovative tissue engineering approaches.

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BMP4 up-regulated by 630 nm LED irradiation is associated with the amelioration of rheumatoid arthritis

Du,

Liu,

et al. 2024

Journal of Photochemistry and Photobiology B: Biology

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Evaluation of the effects of preconditioned human stem cells plus a scaffold and photobiomodulation administration on stereological parameters and gene expression levels in a critical size bone defect in rats

Cited by 7 publications

References 47 publications

MicroRNAs-26 and related osteogenic target genes could play pivotal roles in Photobiomodulation and adipose-derived stem cells-based healing of critical size foot defects in the rat model

MicroRNAs-26 and related osteogenic target genes could play pivotal roles in Photobiomodulation and adipose-derived stem cells-based healing of critical size foot defects in the rat model

Mesenchymal Stem Cells Combined with a P(VDF-TrFE)/BaTiO3 Scaffold and Photobiomodulation Therapy Enhance Bone Repair in Rat Calvarial Defects

BMP4 up-regulated by 630 nm LED irradiation is associated with the amelioration of rheumatoid arthritis

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