Combined transcriptome and metabolome analyses reveal the potential mechanism for the inhibition of Penicillium digitatum by X33 antimicrobial oligopeptide

Lin, Shu-Hua; Wang, Yuanxiu; Lu, Qunlin; Zhang, Bin; Wu, Xiaoyu

doi:10.1186/s40643-021-00472-5

Cited by 8 publications

(3 citation statements)

References 36 publications

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“…Lin et al (2020) found that the X33 oligopeptide produced by Streptomyces lavendulae could affect energy metabolism, oxidative stress, and transmembrane transport, and then inhibit the hyphae polarization of P. digitatum [56]. The transcriptional and metabolome profiling also revealed 3648 DEGs and 190 prominently changed metabolites, which suggested that X33 oligopeptide mainly inhibited P. digitatum growth by affecting cell integrity, genetic information delivery, oxidative stress tolerance, and energy metabolism [57]. Yang et al (2021) reported that, according to RNA-seq analysis, nanoemulsion containing cinnamaldehyde, eugenol or carvacrol mostly affected cellular respiration, proton transmembrane transport and guanyl nucleotide-binding of P. digitatum.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Properties of Cinnamon Bark Oil against Postharvest Pathogen Penicillium digitatum In Vitro

Zhou,

Pan,

Wang

et al. 2024

JoF

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Penicillium digitatum is a major postharvest pathogen that threatens the global citrus fruit industry and causes great economic losses annually. In the present study, inhibitory properties of cinnamon bark oil (CBO) against P. digitatum in vitro were investigated. Results indicated that 0.03% CBO could efficiently inhibit the spore germination, germ tube elongation, mycelial growth, colonial expansion and conidial accumulation of P. digitatum. The results of fluorescein diacetate (FDA) and MitoTraker Orange (MTO) staining also proved the suppression effects of CBO against P. digitatum. Meanwhile, CBO could inhibit green mold rots induced by P. digitatum in citrus fruit when the working concentration of CBO exceeded 0.06%. In addition, the expressions of 12 genes critical for the growth and virulence of P. digitatum were also significantly regulated under CBO stress. Through a transcriptomic analysis, a total of 1802 common differentially expressed genes (DEGs) were detected in P. digitatum after 4 h and 8 h of CBO treatment. Most of the DEG products were associated with carbohydrate, amino acid and lipid metabolism. They directly or indirectly led to the disturbance of the membrane and the generation of reactive oxygen species (ROS). Our results may deepen the understanding of antifungal properties of CBO against P. digitatum and provide the theoretical foundation to uncover the antifungal mechanism of CBO at the molecular level.

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Section: Discussionmentioning

confidence: 99%

Inhibitory Properties of Cinnamon Bark Oil against Postharvest Pathogen Penicillium digitatum In Vitro

Zhou,

Pan,

Wang

et al. 2024

JoF

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“…The resulting P-values were adjusted using the Benjamini-Hochberg method to control the error detection rate. Padj < 0.05 and |log2(foldchange)| > 1 were set as thresholds for significant differential expression [21].…”

Section: Differential Expression Analysismentioning

confidence: 99%

Combined transcriptome and metabolome analysis of a new species of microalgae from Tibetan plateau and its response to sewage treatment

Wang

Zhang

Chen

et al. 2022

Preprint

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Microalgae are pivotal in maintaining water quality in the lakes and rivers of Qinghai-Tibet plateau. The optimum sewage treatment conditions for Desmodesmus sp. are, temperature: 20–25°C, light intensity: 3000–8000 lx, and pH: 7.0–7.5, identified based on orthogonal experiments. The maximum removal rate of total nitrogen, total phosphorus, and chemical oxygen demand was more than 95% in the actual sewage treatment. The sewage treatment capacity of Desmodesmus sp. from plateau is higher than that from plains under the same treatment conditions. To identify the differentially expressed genes and metabolites in Desmodesmus sp. in response to sewage treatment, a combination of metabolomics and transcriptomics was employed to the microalgae with and without sewage treatment. The results showed that the oxidative phosphorylation, photosynthesis, and propanoate metabolism pathways were the most significantly enriched pathways in sewage treatment. Further, the metabolism of adenosine diphosphate, 2-oxobutanoate, and succinate were significantly upregulated, downregulated, and both upregulated and downregulated, respectively, as shown by the combined transcriptome and metabolome analysis. Additionally, we found that sewage treatment could also induce numerous changes in the primary metabolism, such as carbohydrate, fatty acid biosynthesis, and amino acid metabolism when compared with control. Overall, our results should improve fundamental knowledge of molecular responses to Desmodesmus sp. in sewage treatment and contribute to the design of strategies in microalgae response to sewage treatment.

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“…Specifically, P. italicum pathogens with faster mycelium growth are undesirably easy to spread and contaminate healthy citrus fruits even under cold-storage conditions [ 4 ] and create more severe virulence [ 5 ]. Many studies focused on the molecular mechanisms underlying P. digitatum growth; sporulation and virulence, including transcription factors [ 6 , 7 , 8 , 9 , 10 , 11 ]; signaling responses [ 6 , 10 , 12 ]; cell cycle regulation [ 13 , 14 ]; and environmental adaptations [ 12 , 15 , 16 , 17 , 18 , 19 ]. However, such regulation mechanisms are rarely elucidated in P. italicum species.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Functional Analysis of a New Calcium/Calmodulin-Dependent Protein Kinase (CaMK1) in the Citrus Pathogenic Fungus Penicillium italicum

Liu

et al. 2022

JoF

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Calcium (Ca2+)/calmodulin-dependent protein kinases (CaMKs) act as a class of crucial elements in Ca2+-signal transduction pathways that regulate fungal growth, sporulation, virulence, and environmental stress tolerance. However, little is known about the function of such protein kinase in phytopathogenic Penicillium species. In the present study, a new CaMK gene from the citrus pathogenic fungus P. italicum, designated PiCaMK1, was cloned and functionally characterized by gene knockout and transcriptome analysis. The open reading frame of PiCaMK1 is 1209 bp in full length, which encodes 402 amino acid residues (putative molecular weight ~45.2 KD) with the highest homologous (~96.3%) to the P. expansum CaMK. The knockout mutant ΔPiCaMK1 showed a significant reduction in vegetative growth, conidiation, and virulence (i.e., to induce blue mold decay on citrus fruit). ΔPiCaMK1 was less sensitive to NaCl- or KCl-induced salinity stress and less resistant to mannitol-induced osmotic stress, indicating the functional involvement of PiCaMK1 in such environmental stress tolerance. In contrast, the PiCaMK1-complemented strain ΔPiCaMK1COM can restore all the defective phenotypes. Transcriptome analysis revealed that knockout of PiCaMK1 down-regulated expression of the genes involved in DNA replication and repair, cell cycle, meiosis, pyrimidine and purine metabolisms, and MAPK signaling pathway. Our results suggested the critical role of PiCaMK1 in regulating multiple physical and cellular processes of citrus postharvest pathogen P. italicum, including growth, conidiation, virulence, and environmental stress tolerance.

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Combined transcriptome and metabolome analyses reveal the potential mechanism for the inhibition of Penicillium digitatum by X33 antimicrobial oligopeptide

Cited by 8 publications

References 36 publications

Inhibitory Properties of Cinnamon Bark Oil against Postharvest Pathogen Penicillium digitatum In Vitro

Inhibitory Properties of Cinnamon Bark Oil against Postharvest Pathogen Penicillium digitatum In Vitro

Combined transcriptome and metabolome analysis of a new species of microalgae from Tibetan plateau and its response to sewage treatment

Characterization and Functional Analysis of a New Calcium/Calmodulin-Dependent Protein Kinase (CaMK1) in the Citrus Pathogenic Fungus Penicillium italicum

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