Combined transcriptomic/proteomic analysis of salivary gland and secreted saliva in three planthopper species

Huang, Hai‐Jian; Lü, Jiafeng; Li, Qiao; Bao, Yan-Yuan; Zhang, Chuan‐Xi

doi:10.1016/j.jprot.2017.11.003

Cited by 69 publications

(86 citation statements)

References 60 publications

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“…Green shading indicates identified peptides according to LC − MS / MS results in Huang et al . (). Blue shading indicates potential N ‐glycosylation sites and yellow shading indicates potential O ‐glycosylation sites.…”

Section: Resultsmentioning

confidence: 97%

“…Here, we reveal that the planthopper saliva can degrade DNA, and that the responsible enzyme is DNase II. In a previous study, the presence of salivary DNase II has been reported in the white‐backed planthopper (Huang et al ., ; Miao et al ., ), brown planthopper (Liu et al ., ; Huang et al ., ), and SBPH (Huang et al ., ). Although the composition of the saliva has been reported in > 10 Aphididae species, no salivary DNase II was found in these phloem‐feeding aphids (van Bel & Will, ), which deserved further investigation.…”

Section: Discussionmentioning

confidence: 98%

“…We previously identified a high level of DNase II in the saliva of three planthopper species (Huang et al, 2018a). In this study, saliva from 400 SBPH nymphs was pooled, and its DNA catalytic activity was investigated in vitro.…”

Section: Saliva Function As a Dna Scavengermentioning

confidence: 98%

“…In Drosophila melanogaster, DNase II is thought to have roles similar to those in C. elegans (Evans et al, 2002). Surprisingly, DNase IIs have been found to be abundantly and ubiquitously distributed in the saliva of three planthoppers Huang et al, 2018a;Miao et al, 2018) and two spider mite species (Huang et al, 2018b;Zhu et al, 2018). However, the precise functions of these salivary DNase IIs remain unknown.…”

Section: Introductionmentioning

confidence: 99%

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Salivary DNase II from Laodelphax striatellus acts as an effector that suppresses plant defence

et al. 2019

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Summary Extracellular DNA, released by damaged plant cells, acts as a damage‐associated molecular pattern (DAMP). We demonstrated previously that the small brown planthopper (Laodelphax striatellus, SBPH) secreted DNase II when feeding on artificial diets. However, the function of DNase II in insect feeding remained elusive. The influences of DNase II on SBPHs and rice plants were investigated by suppressing expression of DNase II or by application of heterogeneously expressed DNase II. We demonstrated that DNase II is mainly expressed in the salivary gland and is responsible for DNA‐degrading activity of saliva. Knocking down the expression of DNase II resulted in decreased performance of SBPH reared on rice plants. The dsDNase II‐treated SBPH did not influenced jasmonic acid (JA), salicylic acid (SA), ethylene (ET) pathways, but elicited a higher level of H2O2 and callose accumulation. Application of heterogeneously expressed DNase II in DNase II‐deficient saliva slightly reduced the wound‐induced defence response. We propose a DNase II‐based invading model for SBPH feeding on host plants, and provide a potential target for pest management.

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Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 98%

Section: Saliva Function As a Dna Scavengermentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Salivary DNase II from Laodelphax striatellus acts as an effector that suppresses plant defence

et al. 2019

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“…Binding to Gram‐negative bacteria and also strong binding activity to chitin indicate the involvement of this protein in insect immune response. Although this protein protects insects from invasion of microorganisms and to stimulate digestion of food in the mid gut, new studies have found the presence of Peritrophin in insect salivary glands (Baek & Lee, ; Huang et al ., ). Attacin is a glycine‐rich antimicrobial peptide which is differentially expressed in soldier fly salivary glands (Log 2 FC> 3).…”

Section: Resultsmentioning

confidence: 97%

Australian sugarcane soldier fly's salivary gland transcriptome in response to starvation and feeding on sugarcane crops

et al. 2019

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The soldier fly is an endemic pest of sugarcane in Australia. Small numbers of larvae can cause significant damage to roots and reduce the crop yields. Little is known about the composition and function of the soldier fly salivary gland, its secretions, and their roles in insect-plant interactions. In this study, we performed transcriptome analysis of the salivary glands of starved and sugarcane root-fed soldier fly larvae. A total of 31 119 highly expressed assembled contigs were identified in the salivary glands and almost 50% of them showed high levels of similarity to known proteins in Nr databases. Of all the obtained contigs, only 9727 sequences contain an open reading frame of over 100 amino acids. Around 31% of contigs were predicted to encode secretory proteins, including some digestive and detoxifying enzymes and potential effectors. Some known salivary secreted peptides such as serine protease, cysteine proteinase inhibitors, antimicrobial peptides and venom proteins were among the top 100 highly expressed genes. Differential gene expression analysis revealed significant modulation of 850 transcripts in salivary glands upon exposure to plant roots or starvation stress. Here, we identified some venom proteins which were significantly upregulated in the salivary glands of soldier fly larvae exposed to sugarcane roots. In other insects and nematodes some of these proteins have been used to manipulate host plant defense systems and facilitate the invasion of the host plant. These findings provide a further insight into the identification of potential effector proteins involved in soldier fly-sugarcane interactions.

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How does saliva function in planthopper–host interactions?

Huang

Zhang

Hong

2019

Arch Insect Biochem Physiol

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Planthoppers are highly destructive pests that damage rice plants by feeding and transmitting viruses. They feed on phloem sap using specialized mouthparts and secrete saliva during feeding. Over the past decade, genomic, transcriptomic, and proteomic approaches have greatly improved our understanding of the complexity of planthopper saliva, and have provided a glimpse of planthopper-plant interactions. Here we focus on a few recent advances in planthopper saliva and discuss how salivary components influence planthopper performance. Understanding the molecular basis of saliva in planthopper-plant interactions will provide evolutionary insights, and promote the development of novel strategies for controlling agricultural pests. K E Y W O R D S host-plant relationship, planthopper, saliva

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Combined transcriptomic/proteomic analysis of salivary gland and secreted saliva in three planthopper species

Cited by 69 publications

References 60 publications

Salivary DNase II from Laodelphax striatellus acts as an effector that suppresses plant defence

Salivary DNase II from Laodelphax striatellus acts as an effector that suppresses plant defence

Australian sugarcane soldier fly's salivary gland transcriptome in response to starvation and feeding on sugarcane crops

How does saliva function in planthopper–host interactions?

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