Combined Use of Hyperthermia and Irradiation Cause Antiproliferative Activity and Cell Death to Human Esophageal Cell Carcinoma Cells-Mainly Cell Cycle Examination

Yuguchi, Takashi; Saito, Mitsukazu; Yokoyama, Yoshinobu; Saito, Tomohiro; Nagata, Takuya; Sakamoto, Takashi; Tsukada, Kazuhiro

doi:10.1111/j.1749-0774.2002.tb00097.x

Cited by 7 publications

(9 citation statements)

References 12 publications

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“…The cell cycle thus appeared to be arrested at the G2/M phase (24). Yuguchi also observed that hyperthermia could arrest the cell cycle at the G0/G1 phase (25). The MTT results showed that Fe 2 O 3 nanoparticle ferrofluid hyperthermia inhibited the proliferation of SMMC-7721 cells.…”

Section: Discussionmentioning

confidence: 98%

Therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia using Fe2O3 nanoparticles

Yan

Chen

Fan

et al. 2014

Braz J Med Biol Res

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This study aimed to investigate the therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia (MFH) using Fe2O3 nanoparticles. Hepatocarcinoma SMMC-7721 cells cultured in vitro were treated with ferrofluid containing Fe2O3 nanoparticles and irradiated with an alternating radio frequency magnetic field. The influence of the treatment on the cells was examined by inverted microscopy, MTT and flow cytometry. To study the therapeutic mechanism of the Fe2O3 MFH, Hsp70, Bax, Bcl-2 and p53 were detected by immunocytochemistry and reverse transcription polymerase chain reaction (RT-PCR). It was shown that Fe2O3 MFH could cause cellular necrosis, induce cellular apoptosis, and significantly inhibit cellular growth, all of which appeared to be dependent on the concentration of the Fe2O3 nanoparticles. Immunocytochemistry results showed that MFH could induce high expression of Hsp70 and Bax, decrease the expression of mutant p53, and had little effect on Bcl-2. RT-PCR indicated that Hsp70 expression was high in the early stage of MFH (<24 h) and became low or absent after 24 h of MFH treatment. It can be concluded that Fe2O3 MFH significantly inhibited the proliferation of in vitro cultured liver cancer cells (SMMC-7721), induced cell apoptosis and arrested the cell cycle at the G2/M phase. Fe2O3 MFH can induce high Hsp70 expression at an early stage, enhance the expression of Bax, and decrease the expression of mutant p53, which promotes the apoptosis of tumor cells.

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Section: Discussionmentioning

confidence: 98%

Therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia using Fe2O3 nanoparticles

Yan

Chen

Fan

et al. 2014

Braz J Med Biol Res

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“…Compared with cells in the control group, a hypodiploid peak was seen in the pre-G1 phase in cells of the thermotherapy group, which can induce hysteresis in the G2/M phase of the cell cycle. 22 In summary, in the present study, we successfully prepared Fe 3 O 4 paramagnetic nanoparticles. In vitro heating tests confirmed their good heating ability.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of magnetic Fe3O4 nanoparticles and their cytotoxic effect on MCF-7 cells

Chen

Tang²,

Li³

et al. 2012

IJN

146

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Background:The objective of this study was to evaluate the synthesis and biocompatibility of Fe 3 O 4 nanoparticles and investigate their therapeutic effects when combined with magnetic fluid hyperthermia on cultured MCF-7 cancer cells. Methods: Magnetic Fe 3 O 4 nanoparticles were prepared using a coprecipitation method. The appearance, structure, phase composition, functional groups, surface charge, magnetic susceptibility, and release in vitro were characterized by transmission electron microscopy, x-ray diffraction, scanning electron microscopy-energy dispersive x-ray spectroscopy, and a vibrating sample magnetometer. Blood toxicity, in vitro toxicity, and genotoxicity were investigated. Therapeutic effects were evaluated by MTT [3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide] and flow cytometry assays. Results: Transmission electron microscopy revealed that the shapes of the Fe 3 O 4 nanoparticles were approximately spherical, with diameters of about 26.1 ± 5.2 nm. Only the spinel phase was indicated in a comparison of the x-ray diffraction data with Joint Corporation of Powder Diffraction Standards (JCPDS) X-ray powder diffraction files. The O-to-Fe ratio of the Fe 3 O 4 was determined by scanning electron microscopy-energy dispersive x-ray spectroscopy elemental analysis, and approximated pure Fe 3 O 4 . The vibrating sample magnetometer hysteresis loop suggested that the Fe 3 O 4 nanoparticles were superparamagnetic at room temperature. MTT experiments showed that the toxicity of the material in mouse fibroblast (L-929) cell lines was between Grade 0 to Grade 1, and that the material lacked hemolysis activity. The acute toxicity (LD 50 ) was 8.39 g/kg. Micronucleus testing showed no genotoxic effects. Pathomorphology and blood biochemistry testing demonstrated that the Fe 3 O 4 nanoparticles had no effect on the main organs and blood biochemistry in a rabbit model. MTT and flow cytometry assays revealed that Fe 3 O 4 nano magnetofluid thermotherapy inhibited MCF-7 cell proliferation, and its inhibitory effect was dose-dependent according to the Fe 3 O 4 nano magnetofluid concentration. Conclusion: The Fe 3 O 4 nanoparticles prepared in this study have good biocompatibility and are suitable for further application in tumor hyperthermia.

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“…The cells go through G1, S, G2 and M stages in turn during proliferation. It has been reported that the cell cycle is arrested at the G0/G1 phase after hyperthermia 18 . It has been reported by domestic researchers that the tumor cells are arrested at the G1 phase and G2/M phase to a greater or lesser extent after hyperthermia treatment 19,20 .…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that the cell cycle is arrested at the G0/G1 phase after hyperthermia. 18 It has been reported by domestic researchers that the tumor cells are arrested at the G1 phase and G2/M phase to a greater or lesser extent after hyperthermia treatment. 19,20 We used flow cytometry to detect the changes in the cell cycle of Lewis lung cancer cells after hyperthermia treatment.…”

Section: Figurementioning

confidence: 99%

Higher temperature improves the efficacy of magnetic fluid hyperthermia for Lewis lung cancer in a mouse model

Zhang

Liu

et al. 2012

Thoracic Cancer

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Purpose: The aim of this study was to investigate the effect of a higher temperature on the efficacy of magnetic fluid hyperthermia for Lewis lung cancer in a mouse model. Materials and methods: Magnetic fluids were prepared in vitro and directly injected into tumors. Twenty-four hours later, the mice were subjected to an alternating magnetic field. The temperature in the tumor was increased to 46.0°C, higher than the usual temperature used in hyperthermia therapy. The higher temperature was maintained for 30 min with a stable strength of magnetic field. Results: Magnetic fluid hyperthermia with a higher temperature significantly inhibited the growth of the tumors (P < 0.05). The tumors completely regressed in four out of 12 mice. Histological analysis demonstrated that the tumor cells underwent apoptosis and necrosis, and the cells were arrested at the G1/G0 phase of the cell cycle. The lifespan of the treated animals also increased significantly (P < 0.05). Conclusions: Magnetic fluid hyperthermia with a higher temperature could improve the efficacy of this therapy on lung cancer.

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Combined Use of Hyperthermia and Irradiation Cause Antiproliferative Activity and Cell Death to Human Esophageal Cell Carcinoma Cells-Mainly Cell Cycle Examination

Cited by 7 publications

References 12 publications

Therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia using Fe2O3 nanoparticles

Therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia using Fe2O3 nanoparticles

Biocompatibility of magnetic Fe3O4 nanoparticles and their cytotoxic effect on MCF-7 cells

Higher temperature improves the efficacy of magnetic fluid hyperthermia for Lewis lung cancer in a mouse model

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