Combined use of Paracoccidioides brasiliensis recombinant rPb27 and rPb40 antigens in an enzyme-linked immunosorbent assay for immunodiagnosis of paracoccidioidomycosis

Serological Diagnosis of Paracoccidioidomycosis: High Rate of Inter-laboratorial Variability among Medical Mycology Reference Centers

“…Thus, the type of the reaction and antigen preparation may be factors that influence the accuracy of the serological result. Regarding the antigen preparation, not only gp43, but several other components in both the somatic and culture filtrate antigens react with the patients' sera [20], [21], [22]. The amount of these components in the antigen preparations not only varies among the strains, but also in a single strain depending on the number of repeated subculturing, medium used, log phase of growth when the fungus is harvested, among other factors.…”

Profile of total IgG, IgG1, IgG2, IgG3 and IgG4 levels in sera of patients with paracoccidioidomycosis: treatment follow-up using Mexo and rPb27 as antigens in an ELISA

“…It would likely be necessary to use a cocktail of recombinant proteins to achieve higher sensitivity values. Recently, a study performed by our group (Fernandes et al 2011) demonstrated that the combined use of rPb27 and rPb40 in an ELISA provided high sensitivity and specificity for PCM diagnosis, which confirmed that rPb27 was much more efficient as an antigen when paired with another recombinant protein than when isolated.…”

“…It is worth mentioning that the levels of total IgG were high in Rel patients using either Mexo or rPb27 as the antigen. This is an important finding because the majority of followups of PCM patients are performed for total IgG detection and not for its subclasses (Del Negro et al 2000, Fernandes et al 2011). However, this result does not allow for a conclusion about the relationship between relapses and increases in the levels of antibodies because only three patients presented relapses and a statistical determination could not be performed with this small group.…”

“…These techniques may use various antigenic preparations to assess antibodies against P. brasiliensis. Some of these techniques use crude antigenic preparations, while others use specific antigenic preparations, such as the 19 kDa, 31 kDa, 43 kDa and 70 kDa glycoproteins and recombinant Pb27 (rPb27) (Ortiz et al 1998, Baida et al 1999, Díez et al 2003, Albuquerque et al 2005, Correa et al 2007, Reis et al 2008, Fernandes et al 2011, Silveira-Gomes et al 2011. However, there is no consensus on the best techniques for the diagnosis and follow-up care of PCM patients (Campos et al 1990, Alves 1996, Martins et al 1997, Del Negro et al 2000, Camargo 2008).…”

Differential PbP27 expression in the yeast and mycelial forms of the Paracoccidioides brasiliensis species complex

“…Another such antigen is a 27-kDa protein (p27), obtained from a P. brasiliensis cDNA library , with potential use for diagnosis due to a high sensitivity and specificity shown (Diez et al, 2003;Fernandes et al, 2011a;Ortiz et al, 1998Ortiz et al, , 1996. In addition, it has been shown to promote protective immunity in BALB/c mice against infection with P. brasiliensis yeast forms (Fernandes et al, 2011b;Reis et al, 2008), enhancing the PCM antifungal therapy (Fernandes et al, 2011b), and has been suggested as a putative virulence factor (Matute et al, 2008;Ortiz et al, 1996;Reis et al, 2008).…”