Combined Use of the Polymerase Chain Reaction and Detection of Adenosine Deaminase Activity on Pleural Fluid Improves the Rate of Diagnosis of Pleural Tuberculosis

Lima, Danielle Malta; Colares, Jeová Keny Baima; Fonseca, Benedito Antônio Lopes da

doi:10.1378/chest.124.3.909

Cited by 70 publications

(54 citation statements)

References 22 publications

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“…Polymerase chain reaction( PCR), for the detection of mycobacterial deoxyribonucleic acid(DNA), has shown to have a sensitivity of 31.3% and specificity of 96.6% [5] . We treated the child with anti-tubercular chemotherapy with four drug daily regimen for 6 months, in which isoniazid, rifampicin, pyrazinamide, ethambutol was given for 4 months and rifampicin along with isoniazid for 2 months.…”

Section: IIImentioning

confidence: 99%

Pediatric Tubercular Empyema Thoracis: A Diagnostic Dilemma

Najmuddin¹,

Lahiri²,

George³

et al. 2014

IOSRJDMS

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Section: IIImentioning

confidence: 99%

Pediatric Tubercular Empyema Thoracis: A Diagnostic Dilemma

Najmuddin¹,

Lahiri²,

George³

et al. 2014

IOSRJDMS

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“…As recommended, ADA should not be used to replace current diagnostic methods, but as an extra tool in the diagnosis [113].…”

Section: Other Non-microbiological Diagnostic Techniquesmentioning

confidence: 99%

Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field

Palomino¹

2005

Eur Respir J

146

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Tuberculosis (TB) remains one of the major causes of death from a single infectious agent worldwide. Of great concern for TB control is the emergence of drug resistance. Since there is no cure for some multidrug-resistant strains of Mycobacterium tuberculosis, there is concern that they may spread around the world, stressing the need for additional control measures, such as new diagnostics, better drugs for treatment, and a more effective vaccine.Pulmonary TB can be diagnosed by its symptoms, chest radiography, sputum smear microscopy and by cultivation of M. tuberculosis, which is considered as the gold standard. Recent advances in molecular biology and molecular epidemiology, and a better understanding of the molecular basis of drug resistance in TB, have provided new tools for rapid diagnosis; however, the high cost of most of these techniques, and their requirement for sophisticated equipment and skilled personnel have precluded their implementation on a routine basis, especially in low-income countries.Other nonconventional diagnostic approaches proposed include the search for biochemical markers, detection of immunological response and early detection of M. tuberculosis by methods other than colony counting.In the present article, some of these approaches will be reviewed and the feasibility for their implementation in diagnostic laboratories will be discussed.

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“…In the case of TB, one of the most explored is ADA, which is a polymorphic enzyme that catalyzes the deamination of adenosine to inosine and ammonia (6). In numerous studies ADA has proven to be an accurate and valuable test for differential diagnosis of TB (12)(13)(14)(15)(16)(17).…”

Section: Discussionmentioning

confidence: 99%

“…The enzyme catalyzes the hydrolytic and irreversible deamination of adenosine to inosine and deoxyadenosine to deoxyinosine. ADA has been extensively used in the diagnosis of tuberculous pleural effusion (6).…”

Section: Introductionmentioning

confidence: 99%